3g ground samples of the test ingredients were taken and analysed for proximate composition (AOAC, 2005) . Gross energy was estimated using the adiabatic bomb calorimeter (Model 1261; Parr Instrument Co., Moline, IL, USA). Analysis of mineral nutrients was determined according to the method by Kumari et al. (2017) (link). The quantitative analysis was carried out by using inductively coupled plasma atomic absorption emission spectroscopy (ICP-AES). Samples were also analysed for mineral contents using atomic absorption spectrophotometer (Perkin Elmer Optima 4300DV ICP spectrophotometer, UK). The phytochemical constituents were analysed following standard procedures; total polyphenol (Wright et al., 2000) , avonoid (Arvouet-Grand et al., 1994) , extractable tannin (Hoff and Singleton, 1977) and saponin content (Edeoga et al., 2005) .
Optima 4300dv icp spectrophotometer
Manufactured by PerkinElmer
Sourced in United Kingdom
The Optima 4300DV ICP spectrophotometer is an inductively coupled plasma optical emission spectrometer (ICP-OES) designed for elemental analysis. It utilizes a dual-view optical system to provide both axial and radial plasma viewing for a wide range of detection capabilities. The Optima 4300DV is a versatile instrument capable of analyzing a variety of sample types, including aqueous solutions, slurries, and digests.
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Lab products found in correlation
4 protocols using optima 4300dv icp spectrophotometer
1
Comprehensive Nutrient Analysis of Test Ingredients
2
Copper Content Analysis of Poultry Feed
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The research work was carried out at the Poultry Unit of the Directorate of University Farms (DUFARM), Federal University of Agriculture, Alabata, 1 Abeokuta, Nigeria (Latitude 7° 13 49. 46 The dietary ingredients were dried at 65 C for 36 hours in an oven and ground to pass through 1mm sieve and were analysed for dry matter (DM), crude fibre (CF), ether extract (EE), and total ash (AOAC, 1995, ID 7.101, 7.048, 7.016) . The nitrogen fraction of feed samples was determined using kjeldahl destruction method and crude protein (CP) was determined by multiplying the N value by 6.25. The copper content of the basal diet was estimated by o igniting the feed sample at 400 C for 4 hours in a muffle furnace. The ash was reconstituted using wet-ashing procedure (James, 1996) . Analysis of Cu was estimated by using a Perkin Elmer Optima 4300DV ICP spectrophotometer (Perkin Elmer, Beaconsfield, UK)
3
Copper Bioaccumulation in Livestock Tissues
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Livers, kidneys, hearts, lungs and tissues from thigh and breast were excised and o stored at -20 C. The samples were dried at o 100 C for 36hrs and digested using a modified wet-ashing procedure (James, 1996) . Ash was reconstituted in 5ml of 1 N HCl solution and analyzed for copper Perkin Elmer Optima 4300DV ICP spectrophotometer (Perkin Elmer, Beaconsfield, UK) Data Collection Data on growth indices such as body weight, weight gain, feed intake and feed conversion ratio were collected weekly Experimental Design and Statistical Analysis The experimental layout was 2 (sources of copper) x 3 (concentration of copper) factorial arrangements. The data obtained were subjected to analysis of variance in completely randomized design using MINITAB statistical software (MINITAB, 2000) . Significant means among variables were separated using Duncan Multiple Range Test of the same package at 5% level of significance .
4
Characterization of Malted Sorghum Sprouts
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Malted sorghum sprouts (MSP) was obtained commercially from a local brewery industry in Ogun State, Nigeria. This was dried (10-11% moisture content) prior to collection and included on DM basis in the experimental diets. Proximate composition (AOAC, 1990) , fibre fractions (Van Soest et al., 1991) , gross energy (Adiabatic bomb calorimeter, Parr Instrument Company, Moline, IL, USA) and tannin content (Hoff and Singleton, 1977) of MSP was determined according to standard procedures (Table 1). For Ca and P determination of MSP, samples were further dried in a hot air oven (105 o C for 8 h), milled to pass through 0.5 mm sieve and ignited at 400 o C for 4 h in a muffle furnace. The ash was treated with HNO 3 under mild heat (80 o C) and digested (15ml HNO 3 ). Analysis was done using the atomic absorption spectrophotometer (Perkin Elmer Optima 4300DV ICP spectrophotometer, Beaconsfield, UK).
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