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Milliplex map human adipocyte magnetic bead panel endocrine multiplex assay

Manufactured by Merck Group

The MILLIPLEX MAP Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay is a laboratory equipment product developed by Merck Group. It is a multiplex assay designed to simultaneously measure multiple analytes related to adipocyte and endocrine function in a single sample. The assay utilizes magnetic beads coated with specific capture antibodies to detect and quantify the target analytes.

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2 protocols using milliplex map human adipocyte magnetic bead panel endocrine multiplex assay

1

Quantifying Adipokine Profiles in ASCs

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Fresh conditioned media was collected from culture dishes, and debris/cells were removed by centrifugation and 0.45 μm filters. PAI-1 levels were measured along with other adipokines and cytokines (n = 11) by using MILLIPLEX MAP Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay (MilliporeSigma, Cat# HADCYMAG-61K) following the manufactures instructions. The values were detected by Luminex® 200 multiplexing system (MilliporeSigma). Levels of PAI-1 and other cytokines/adipokines were calculated in reference to a standard curve and normalized to total proteins from ASCs in cell culture.
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2

Quantifying Adipokine Profiles in ASCs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Fresh conditioned media was collected from culture dishes, and debris/cells were removed by centrifugation and 0.45 μm filters. PAI-1 levels were measured along with other adipokines and cytokines (n = 11) by using MILLIPLEX MAP Human Adipocyte Magnetic Bead Panel - Endocrine Multiplex Assay (MilliporeSigma, Cat# HADCYMAG-61K) following the manufactures instructions. The values were detected by Luminex® 200 multiplexing system (MilliporeSigma). Levels of PAI-1 and other cytokines/adipokines were calculated in reference to a standard curve and normalized to total proteins from ASCs in cell culture.
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