Log In Sign up
The largest database of trusted experimental protocols

Rosetta 2 de3 competent e coli cells

Manufactured by Merck Group
Visit Supplier

Rosetta 2(DE3) competent E. coli cells are a laboratory strain of Escherichia coli bacteria that are engineered to enhance the expression of proteins from organisms with codon usage patterns that differ from standard E. coli. They are designed to improve the solubility and folding of recombinant proteins expressed in E. coli.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

E. coli Rosetta 2(DE3) Singles Competent Cells E. coli Rosetta 2(DE3) cells Rosetta 2(DE3) Competent Cells E. coli Rosetta 2 (DE3) Rosetta™ (DE3) competent cells E. coli Rosetta 2 cells E. coli Rosetta cells Rosetta 2(DE3) cells Rosetta (DE3) cells Rosetta Competent Cells

2 protocols using rosetta 2 de3 competent e coli cells

1

Recombinant HCMV Protease Expression

Check if the same lab product or an alternative is used in the 5 most similar protocols
An N-terminal His6-tagged construct (Uniprot A0A0C7TBJ5, AA 1–235) was transformed into Rosetta 2(DE3) competent E. coli cells (MilliporeSigma). Cells were grown and induced using the same procedure described for unlabeled HCMV Pr.
Hulce K.R., Jaishankar P., Lee G.M., Bohn M.F., Connelly E.J., Wucherer K., Ongpipattanakul C., Volk R.F., Chuo S.W., Arkin M.R., Renslo A.R, & Craik C.S. (2022). Inhibiting a dynamic viral protease by targeting a non-catalytic cysteine. Cell chemical biology, 29(5), 785-798.e19.
+ Open protocol
+ Expand
2

Stabilized HCMV Protease Purification

Check if the same lab product or an alternative is used in the 5 most similar protocols
All HCMV Pr constructs bear a N-terminal His6 tag. WT HCMV Pr and all subsequent mutant constructs contain A141V, A143V, P144A and A209V mutations to stabilize against autoproteolysis. Constructs were expressed in Rosetta 2(DE3) competent E. coli cells (MilliporeSigma).
Hulce K.R., Jaishankar P., Lee G.M., Bohn M.F., Connelly E.J., Wucherer K., Ongpipattanakul C., Volk R.F., Chuo S.W., Arkin M.R., Renslo A.R, & Craik C.S. (2022). Inhibiting a dynamic viral protease by targeting a non-catalytic cysteine. Cell chemical biology, 29(5), 785-798.e19.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!