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Pulse software

Manufactured by HEKA Elektronik
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Sourced in Germany

Pulse software is a data acquisition and analysis tool developed by HEKA Elektronik. It provides a platform for recording and processing experimental data from various laboratory equipment.

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Lab products found in correlation

Epc 10 amplifier, by HEKA Elektronik (14 mentions) Epc 10 patch clamp amplifier, by HEKA Elektronik (7 mentions) Epc 9 amplifier, by HEKA Elektronik (6 mentions) Epc 10, by HEKA Elektronik (3 mentions) Patchmaster software, by HEKA Elektronik (2 mentions) Igor software, by Wavemetrics (2 mentions) Prism 5, by GraphPad (2 mentions) P 97 micropipette puller, by Sutter Instruments (2 mentions) Borosilicate glass, by World Precision Instruments (1 mentions) Sutter p 97 horizontal puller, by Sutter Instruments (1 mentions) Heka epc 10 amplifier, by HEKA Elektronik (1 mentions) Sutter p 97 puller, by Sutter Instruments (1 mentions) Patchmaster, by HEKA Elektronik (1 mentions) Axopatch 200b amplifier, by Molecular Devices (1 mentions) Epc 8 patch clamp amplifier, by HEKA Elektronik (1 mentions) Diaphot, by Nikon (1 mentions) Epc10 2 amplifier, by HEKA Elektronik (1 mentions) Epc 9, by HEKA Elektronik (1 mentions) Rc 22c, by Warner Instruments (1 mentions) Tw150f 3, by World Precision Instruments (1 mentions)

39 protocols using pulse software

1

Whole-cell Patch-clamp Recording in Pancreatic Islets

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Whole-cell currents were recorded in intact islets using the standard whole-cell configuration. Measurements were performed using an EPC-10 patch-clamp amplifier and Pulse software (HEKA Electronics, Lambrecht/Pfalz, Germany). Currents were filtered at 2.9 kHz and digitized at more than 10 kHz. Currents were compensated for capacitive transients and leak current subtraction was conducted. The extracellular medium consisted of (mM) 118 NaCl, 20 tetraethylammonium-Cl (TEA-Cl), 5.6 KCl, 1.2 MgCl2, 5 HEPES (pH 7.4 with NaOH), 2.6 CaCl2 and 1 d-glucose. Two intracellular (pipette) solutions were used (solution 1 and solution 2). Solution 1 contained (mM) 125 K-glut, 10 KCl, 10 NaCl, 1 MgCl2, 5 HEPES, 3 MgATP and 0.05 EGTA (KOH buffered). Solution 2 contained 15 Cs-glut, 10 CsCl, 10 NaCl, 1 MgCl2, 5 HEPES, 3 MgATP, 0.05 EGTA (CsOH buffered). All chemicals were from Sigma-Aldrich. Only recordings with an access resistance of less than 50 MΩ were used for analysis.
Briant L.J., Zhang Q., Vergari E., Kellard J.A., Rodriguez B., Ashcroft F.M, & Rorsman P. (2017). Functional identification of islet cell types by electrophysiological fingerprinting. Journal of the Royal Society Interface, 14(128), 20160999.
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2

Patch-Clamp Measurements of β-Cell Calcium Currents

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For patch-clamp measurements, islets were dissociated into single β-cells. Insulin-secreting β-cells were identified based on their larger size reflected by their higher capacitance (>5.5 pF) and complete inactivation of the Na+ current at -70 mV [10 (link)]. Whole-cell Ca2+ currents (using Ba2+ as the charge carrier to prevent Ca2+-mediated inactivation of the channels and to increase the magnitude of the current) and exocytosis (Ca2+ present in the extracellular solution) were recorded using an EPC-10 amplifier and the Pulse software (Heka Electronics, Lamprecht/Pfalz, Germany) as described previously [11 (link)].
Quinault A., Leloup C., Denwood G., Spiegelhalter C., Rodriguez M., Lefebvre P., Messaddeq N., Zhang Q., Dacquet C., Pénicaud L, & Collins S.C. (2018). Modulation of large dense core vesicle insulin content mediates rhythmic hormone release from pancreatic beta cells over the 24h cycle. PLoS ONE, 13(3), e0193882.
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3

Whole-cell Patch Clamping of Ion Channels

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Whole-cell patch clamping was performed at room temperature using a computer-based patch-clamp amplifier (EPC-9, HEKA Electronics, Reutlingen, Germany) and PULSE software (HEKA Electronics). The bath solution contained (mM): NaCl 140; KCl 4; CaCl2 2; MgCl2 2; glucose 10; and HEPES, 10; adjusted to pH 7.4 with NaOH. The internal solution contained (mM): K gluconate 80; KCl 40; CaCl2 2; EGTA 5; MgATP 2; and HEPES 10; adjusted to pH 7.3 with KOH. Patch pipettes were pulled from borosilicate glass and fire-polished. When filled with the internal solution, pipette resistance ranged between 2 and 4 MΩ. Series resistance did not exceed 10 MΩ and was compensated by 50–70%. Acquired currents were filtered at 2.7 kHz and sampled at 10 kHz. All voltages shown are nominal and have not been corrected for the liquid junction potential. The holding potential was −75 mV throughout. Cell capacitance was compensated automatically by the EPC-9 amplifier.
Liu D., Rychkov G., Hurtado P., Luo H.Y., Zhang T., Bobrovskaya L, & Zhou X.F. (2022). Conversion of Human Fibroblasts into Induced Neural Stem Cells by Small Molecules. International Journal of Molecular Sciences, 23(3), 1740.
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4

Voltage-Clamp Recording of Potassium Currents

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Potassium currents were recorded under voltage-clamp with an EPC10 patch clamp amplifier interface (HEKA Electronics, Lambrecht, Pfalz, Germany). For data acquisition, pulse generation, and analysis, a computer (Dell, Round Rock, TX, USA) running PULSE software (HEKA Electronics) was used. All experiments were conducted at room temperature (20–24 °C). Data are presented as means ± SE of three experiments. The patch pipettes were made from Corning type 7052 glass (Garner Glass, Claremont, CA, USA). Whole-cell currents were obtained conventionally from dialyzed cells voltage-clamped through ruptured membrane patches. The solution bathing the cells contained: 140 mM NaCl, 10 mM HEPES, 1 mM CaCl2, 1 mM MgCl2, 5 mM KCl, and 10 mM glucose, having the pH adjusted to 7.4 saline, and was superfused at 1 mL/min. The pipette solution contained 140 mM KCl, 2 mM MgCl2, 1 mM CaCl2, 10 mM HEPES, and 2.5 mM EGTA (~50 nM free Ca2+), with the pH adjusted to 7.2. The pulse protocol was run a minimum of 10 times before the perfusion with (+)-strebloside to ensure that the currents were stable.
Chen W.L., Ren Y., Ren J., Erxleben C., Johnson M.E., Gentile S., Kinghorn A.D., Swanson S.M, & Burdette J.E. (2017). (+)-Strebloside-induced Cytotoxicity in Ovarian Cancer Cells is Mediated through Cardiac Glycoside Signaling Networks. Journal of natural products, 80(3), 659-669.
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5

Whole-cell Na+ Currents in Pancreatic Islet Cells

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Whole-cell Na+ currents were recorded in α- and β-cells within intact islets using the standard whole-cell configuration as previously described (Gopel et al. 1999 (link)). The measurements were performed using EPC-9 or EPC-10 patch-clamp amplifiers and Pulse software (HEKA Electronics, Lambrecht/Pfalz, Germany). Currents were compensated for capacitive transients and linear leak using a −P/4 protocol. The currents were filtered at 2.9 kHz and digitised at >10 kHz.
The standard extracellular medium for the electrophysiological measurements consisted of (mm) 118 NaCl, 20 tetraethylammonium-Cl (TEA-Cl), 5.6 KCl, 1.2 MgCl2, 5 Hepes (pH 7.4 with NaOH), 2.6 CaCl2, 5 d-glucose and 2 CoCl2 (to block Ca2+ channels). The pipette solution contained (mm) 120 CsCl, 1 MgCl2.6H2O, 1 CaCl2, 10 EGTA, 10 Hepes (pH 7.15 with CsOH) and 3 Mg-ATP. TTX (Alomone Labs, Jerusalem, Israel) was used at a final concentration of 0.1 μg ml−1.
Membrane potential recordings were performed as described previously (De Marinis et al. 2010 (link)) using the perforated-patch technique and K2SO4-filled electrodes. In these experiments, the extracellular (Krebs–Ringer buffer, KRB) solution consisted of (mm) 140 NaCl, 3.6 KCl, 0.5 MgSO4, 0.5 NaH2PO4, 2 NaHCO3, 5 Hepes, 1.5 CaCl2 and glucose as indicated. All electrophysiological experiments were performed at 34°C.
Zhang Q., Chibalina M.V., Bengtsson M., Groschner L.N., Ramracheya R., Rorsman N.J., Leiss V., Nassar M.A., Welling A., Gribble F.M., Reimann F., Hofmann F., Wood J.N., Ashcroft F.M, & Rorsman P. (2014). Na+ current properties in islet α- and β-cells reflect cell-specific Scn3a and Scn9a expression. The Journal of Physiology, 592(Pt 21), 4677-4696.
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6

Electrophysiology of Glucose-Exposed GlyR-HEK Cells

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Current responses from GlyR-transfected HEK293 cells were measured at room temperature (21–23°C) at a holding potential of −50 mV using a HEKA EPC10 amplifier (HEKA Electronics, Lambrecht, Germany) controlled by Pulse software (HEKA Electronics). Recording pipettes were pulled from borosilicate glass (World Precision Instruments, Berlin, Germany) using a Sutter P-97 horizontal puller (Sutter, Novato, CA, USA). Solutions were applied using an Octaflow system (NPI electronics, Tamm, Germany). The external buffer consisted of 135 mM NaCl, 5.5 mM KCl, 2 mM CaCl2, 1.0 mM MgCl2, and 10 mM Hepes (pH adjusted to 7.4 with NaOH); the internal buffer was 140 mM CsCl, 1.0 mM CaCl2, 2.0 mM MgCl2, 5.0 mM EGTA, and 10 mM Hepes (pH adjusted to 7.2 with CsOH). Glucose (Sigma-Aldrich, Munich, Germany) was added to the growth medium on the day before experiments to give a pre-exposure to 50 mM of Glucose for 16–20 h. Dose-response data were fitted to the Hill equation (see Supplementary Material) to determine EC50 and IC50. Significance of differences between EC50 values were determined using one-way ANOVA with p ≤ 0.05 (*) and p ≤ 0.01 (**) taken as significant.
Hussein R.A., Ahmed M., Breitinger H.G, & Breitinger U. (2019). Modulation of Glycine Receptor-Mediated Pain Signaling in vitro and in vivo by Glucose. Frontiers in Molecular Neuroscience, 12, 280.
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7

Whole-Cell Patch Clamp Measurement of Kir2.1

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The mature myocardial cells were digested into single cells with digestive enzymes 2 days in advance and adhere to the cover glasses. The whole-cell patch clamp experiment was conducted to measure the Cm with a single beat at 32–35 °C through the EPC-10 patch clamp amplifier (HEKA Electronics, Lambrecht, Germany). The whole-cell Kir2.1 potassium current was recorded with the help of an EPC-10 patch clamp amplifier controlled by PULSE software (HEKA Electronics). At the same time, PatchMaster software (HEKA Electronics) was used to capture data.
Chen P., Long J., Hua T., Zheng Z., Xiao Y., Chen L., Yu K., Wu W, & Zhang S. (2024). Transcriptome and open chromatin analysis reveals the process of myocardial cell development and key pathogenic target proteins in Long QT syndrome type 7. Journal of Translational Medicine, 22, 307.
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8

Whole-cell patch-clamp recordings of TRPM3 currents

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Whole-cell patch-clamp recordings were performed with an EPC-10 amplifier and PULSE software (HEKA Electronics, Lambrecht) at RT22 (link),59 . Currents were recorded with glass pipettes (1–3 MΩ resistance) fabricated from borosilicate glass capillaries using a Sutter P-97 puller (Sutter Instruments, Novato, CA). Voltage errors were minimised using 80-90% series resistance compensation. TRPM3 currents in DRG neurons were recorded with a ramp potential of −75 mV to +75 mV. The voltage for current analysis was ±75 mV. Neurons with a leak current of >500 pA or a series resistance of >10 MOhm were excluded. The extracellular solution contained (in mM) 140 NaCl, 3.5 KCl, 1 MgCl2, 1.25 NaH2PO4, 10 HEPES, and 10 D-glucose (adjusted to pH 7.4 with NaOH). The pipette solution contained (in mM) 140 CsCl, 1 CaCl2, 2 MgATP, 2 Na2ATP, 10 EGTA, and 5 TEA-Cl (adjusted to pH 7.2 with CsOH). The osmolality of all solutions was adjusted to 310 mOsm.
Xie M.X., Cao X.Y., Zeng W.A., Lai R.C., Guo L., Wang J.C., Xiao Y.B., Zhang X., Chen D., Liu X.G, & Zhang X.L. (2021). ATF4 selectively regulates heat nociception and contributes to kinesin-mediated TRPM3 trafficking. Nature Communications, 12, 1401.
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9

Patch-Clamp Measurement of Beta-Cell Exocytosis

Cited 2 times
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For patch-clamp measurements, islets were dissociated into single β–cells. In whole-cell measurements, insulin-secreting β-cells were identified based on their larger size (>5.5 pF) and complete inactivation of the Na+ current at -70 mV (see ref. no (16) (link)). Exocytosis and whole-cell Ca2+ currents were recorded using an EPC-10 amplifier and the Pulse software (Heka Electronics, Lamprecht/Pfalz, Germany) as described previously (15 (link)). Single exocytotic events and fusion pore expansion were detected in the cell-attached configuration. The standard extracellular solution described in (16 (link)) supplemented with 20 mM glucose was used as the pipette-filling medium. The PATCHMASTER software (Heka electronics) together with the EPC10 implements an 'internal calibration'. They automatically correct for phase-shifts and frequency-dependent attenuation when a sinusoidal voltage command of 25 kHz is generated (17 (link)). The scaled apparent capacitance (Im/ω) and conductance (Re) are then calculated online by the software.
Collins S.C., Do H.W., Hastoy B., Hugill A., Adam J., Chibalina M.V., Galvanovskis J., Godazgar M., Lee S., Goldsworthy M., Salehi A., Tarasov A.I., Rosengren A.H., Cox R, & Rorsman P. (2016). Increased expression of the diabetes gene SOX4 reduces insulin secretion by impaired fusion pore expansion. Diabetes, 65(7), 1952-1961.
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10

Electrophysiological Recording of Pancreatic Beta Cells

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Electrical activity was recorded from single β-cells freshly isolated from islets using the perforated patch whole-cell configuration. β-cells were functionally identified by the absence of a voltage-gated Na+-currents when using a holding potential of −70mV, and a larger whole-cell membrane capacitance (≥7 pF) [23] (link). The pipette resistance ranged between 2 and 5 MΩ when the pipettes were filled with the intracellular solutions (mM): 128 K-gluconate, 10 KCl, 10 NaCl, 1 MgCl2, 5 HEPES (pH 7.3, adjusted with KOH). The extracellular medium consisted of (in mM): 140 NaCl, 5 NaHCO3, 3.6 KCl, 0.5 NaH2PO4, 1.5 CaCl2, 0.5 mM MgSO4, 10 HEPES and 5 mM d-glucose (pH 7.4, adjusted with NaOH). All experiments were conducted using an EPC-10 patch-clamp amplifier and the PULSE software (HEKA Electronics). Data were filtered and sampled with Pulse/Pulsefit and software (Heka Electronik). All electrophysiological experiments were performed at 20–25 °C.
Kong X., Tu Y., Li B., Zhang L., Feng L., Wang L., Zhang L., Zhou H., Hua X, & Ma X. (2019). Roux-en-Y gastric bypass enhances insulin secretion in type 2 diabetes via FXR-mediated TRPA1 expression. Molecular Metabolism, 29, 1-11.
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