The pure culture of each isolate was grown in MRS broth for 24 h and cell free supernatant (CFS) was obtained by centrifuging at 10,000 rpm, 4 °C, for 10 min. The collected CFS was checked for their antibacterial activity against Enterococcus faecalis MTCC 439, Shigella flexneri MTCC 1457, Aeromonas hydrophila MTCC 1739, Escherichia coli MTCC 40, Pseudomonas aeruginosa MTCC 4679, Staphylococcus aureus MTCC 737, Klebsiella pneumonaie MTCC 39, and Proteus vulgaris MTCC 426 received from Microbial Type Culture Collection (MTCC), Chandigarh, India and clinical pathogens such as P. aeruginosa, K. pneumoniae, Acinetobacter baumannii, methicillin resistant S. aureus (MRSA) received from Mahathma Gandhi Memorial Government Hospital, Tiruchirappalli, Tamil Nadu, India. The assay was performed by well diffusion method using 50 µL of each indicator bacteria (2.1 × 105 CFU/mL).
K pneumoniae
Manufactured by Microbial Type Culture Collection
Sourced in India
K. pneumoniae is a bacterium that can be used in microbial culture collections. It is a gram-negative, rod-shaped bacterium.
Automatically generated - may contain errors
Lab products found in correlation
P aeruginosa, by Microbial Type Culture Collection (2 mentions)
S aureus, by Microbial Type Culture Collection (2 mentions)
E coli, by Microbial Type Culture Collection (2 mentions)
Enterococcus faecalis mtcc 439, by Microbial Type Culture Collection (1 mentions)
Shigella flexneri mtcc 1457, by Microbial Type Culture Collection (1 mentions)
Escherichia coli mtcc 40, by Microbial Type Culture Collection (1 mentions)
Pseudomonas aeruginosa mtcc 4679, by Microbial Type Culture Collection (1 mentions)
Staphylococcus aureus mtcc 737, by Microbial Type Culture Collection (1 mentions)
4 protocols using k pneumoniae
1
Antibacterial Activity of Probiotic Cultures
2
Antimicrobial Efficacy of Zinc Oxide Solutions
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The bacterial strains such as E. coli, S. aureus, K. pneumoniae, and S. aeruginosa were purchased from Microbial Type Culture Collection, Chandigarh, India. Actively growing test bacterial strains were spread on four wells made in the nutrient agar plate. The zinc oxide solution with different concentrations such as 50, 100, and 150 µg/ml was loaded in each well, while one well was filled with only broth medium as a control. Then, the plates were incubated at 37°C for 24-48 h. Antimicrobial activity was expressed as a diameter (mm) of the inhibitory zone.
3
Bacterial Strain Culturing and Maintenance
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The bacterial strains used for the current study K. pneumoniae (MTCC no. 432) and S. marcescens (MTCC no. 2645) cultures were procured from Microbial Type Culture Collection and Gene Bank (MTCC), Chandigarh, India. The cultures were allowed to grow and maintained on growth medium-3 (GM-3) liquid broth and 2% agar and incubated at 37 °C, 120 rpm.
4
Antibacterial Activity Evaluation of Microbial Strains
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Five bacterial strains viz., P. aeruginosa (MTCC424), E. coli (MTCC739), K. pneumoniae (MTCC139), S. typhi (MTCC3224), and S. aureus (MTCC96) were procured from the Microbial Type Culture Collection (MTCC), Chandigarh (India). The strains included both gram-negative as well as gram-positive strains; for agar well diffusion assay, all strains were initially sub-cultured in nutrient agar media and incubated at 37 °C for 18 ± 2 h. The MIC for all strains was determined by the broth dilution method for which they were grown at 37 °C for 18 ± 2 h in MHB. For antibacterial assay, gentamycin (10 μg mL–1) and DMSO were used as positive and negative controls whereas for MIC, plant extract and inoculated broth were used as positive and negative controls.
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