C glabrata atcc 2001

The reference strain C. glabrata ATCC 2001 was used in this study (American Type Culture Collection, Manassas, VA, USA). Standard culture media were used, including YPD (Becton, Dickinson and Company, Franklin Lakes, NJ, USA): yeast extract (1%, w/v), peptone (2%, w/v), glucose (2%, w/v), agar (1.5%, w/v), and yeast nitrogen base (YNB) without amino acids (Becton, Dickinson and Company, USA): yeast nitrogen base (0.67%, w/v), ammonium sulfate (0.5%, w/v). Synthetic complete (SC) media were prepared with YNB without amino acids, supplemented with complete supplement mixture (0.2%, w/v) (Formedium, Hunstanton, UK), glucose (2%, w/v), and agar (2%, w/v). In addition, glucose was replaced with alternative carbon sources: acetate (2%, w/v), lactate (2%, v/v), ethanol (2%, v/v), glycerol (2%, v/v), or oleic acid (0.2%, w/v) (Sigma-Aldrich, St. Louis, MO, USA) as the sole carbon source in SC media [2 (link),51 (link)].

The reference strains C. albicans ATCC 90028, C. krusei ATCC 6258 and C. glabrata ATCC 2001 (American Type Culture Collection) were kindly provided by the Faculdade de Odontologia de Araraquara, Universidade do Estado de São Paulo. Three C. albicans clinical isolates from the oral cavity of HIV+ patients (Oral 37 HIV+, Oral 38 HIV⁺, and Oral 40 HIV⁺), the isolates from the oral cavity (Oral 01 HIV⁺C. parapsilosis and Oral 22 C. krusei), and vaginal smears of patients with vulvovaginal candidiasis (Vaginal 11 C. glabrata and Vaginal 14 C. glabrata) were donated by the microorganism collection sector of the Applied Microbiology Laboratory of Universidade CEUMA (UNICEUMA).