Rom 308

Mice were intravenously administered Toc-HDO-antimiR #7 at 5 mg/kg, 15 mg/kg, or 50 mg/kg. Blood samples were collected for serum biochemical analysis at 7 days after injection. Kidneys and livers were removed for histological analysis at 7 days after injection of Toc-HDO-antimiR #7 at 50 mg/kg. Kidney and liver tissues were fixed by transcardiac perfusion with 4% paraformaldehyde, and immersed in 4% paraformaldehyde overnight. The tissues were embedded in paraffin, and then were cut into 5-μm sections by using a microtome (ROM-308; Yamato Kohki Industrial Co, Tokyo, Japan). The sections were stained with hematoxylin and eosin (Muto Pure Chemicals).

Explanted materials were processed by a standard protocol. Samples were fixed in 10% neutral-buffered formalin solution for 1 week, demineralized in 10% formic acid for 4 weeks, and embedded in paraffin. Prepared paraffin block specimens were sectioned into 5 µm thickness on a microtome (Yamato ROM 308, Tokyo, Japan). Hematoxylin and eosin (HE)-stained sections were observed by an optical microscope (Nikon ECLIPSE 80i, Nikon Corporation, Tokyo, Japan) and photographs were taken.