The human SCLC cell lines NCI-H841, NCI-H1048, NCI-889, NCI-2171, NCI-146, NCI-H1963, HCC33 and NCI-H211 were obtained from the American Type Culture Collection (Manassas, VA, USA). Cells were grown in RPMI 1640 with 10% FetalClone (Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C with 5% CO2 in a humidified incubator, except for NCI-H2171 and NCI-H889 cells, which were grown in a HITES medium (DMEM/F12 supplemented with 1% Glutamax, 100 U/mL penicillin, 100 µg/mL streptomycin, 4 µg/mL hydrocortisone (Sigma, St Louis, MO, USA), 5 ng/mL murine EGF and 1% of an insulin–transferrin–selenium mix (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) with 5% FetalClone. Cell lines were authenticated and routinely tested for mycoplasma.
Nci 146
Manufactured by American Type Culture Collection
Sourced in United States
The NCI-146 is a laboratory equipment product designed for cell culture applications. It provides a controlled environment to support the growth and maintenance of cells in vitro. The core function of the NCI-146 is to maintain consistent temperature, humidity, and gas levels within the enclosed chamber to enable optimal cell culture conditions.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Hydrocortisone, by Merck Group (1 mentions)
Insulin transferrin selenium mix, by Thermo Fisher Scientific (1 mentions)
Nci h211, by American Type Culture Collection (1 mentions)
Nci h841, by American Type Culture Collection (1 mentions)
Nci h1048, by American Type Culture Collection (1 mentions)
Hcc33, by American Type Culture Collection (1 mentions)
Hcc1419, by American Type Culture Collection (1 mentions)
Hutu80, by American Type Culture Collection (1 mentions)
Mda mb 231, by American Type Culture Collection (1 mentions)
U118mg, by American Type Culture Collection (1 mentions)
Calu 3, by American Type Culture Collection (1 mentions)
Nci h661, by American Type Culture Collection (1 mentions)
Nci h522, by American Type Culture Collection (1 mentions)
Nci h716, by American Type Culture Collection (1 mentions)
Sk n mc, by American Type Culture Collection (1 mentions)
Universal mycoplasma detection kit, by American Type Culture Collection (1 mentions)
Caov4, by American Type Culture Collection (1 mentions)
Ovsaho, by Japanese Collection of Research Bioresources Cell Bank (1 mentions)
2 protocols using nci 146
1
Culturing SCLC Cell Lines for Research
2
Cell Line Sourcing and Mycoplasma Testing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The cell lines NCI-H661, NCI-H522, NCI-146, KLE, Caov4, NCI-H716, HuTu80, HCC 1419, Calu-3, 22Rv1, U-118MG, A-673, SK-N-MC, MDA-MB-231, A549, K-562, L Wnt-5A, and L cells were obtained from the American Type Culture Collection (ATCC). The cells, Cov434 (cat. no. 07071909), COV318 (cat. no. 07071903), and 1321N1 (cat. no. 86030402) were obtained from Sigma, HEC-59 (cat. no. C0026001) from AddexBio and OVKATE and OVSAHO from the Japanese Collection of Research Bioresources (JCRB) Cell Bank. All cells were tested for mycoplasma contamination before use with the Universal Mycoplasma Detection Kit (ATCC 30-1012K).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!