Mcp100 polarimeter

Manufactured by Anton Paar

Sourced in Austria

The MCP100 polarimeter is a laboratory instrument designed to measure the optical rotation of a sample. It determines the angle of rotation of the plane of polarized light passing through the sample, providing information about the sample's optical activity. The MCP100 is a precise and reliable tool for various applications in the fields of chemistry, pharmaceuticals, and material science.

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Lab products found in correlation

Ascend 600 mhz nmr spectrometer, by Bruker (2 mentions) Maxis quadrupole time of flight mass spectrometer, by Bruker (2 mentions) Lc 16p system, by Shimadzu (2 mentions) Analytical and hplc grade reagents, by Maclin Biochemical Technology (2 mentions) Cryoprobe, by Bruker (2 mentions) Xevo g2 q tof mass spectrometer, by Waters Corporation (2 mentions) Lh 20, by Cytiva (1 mentions) Xevo g2 qtof, by Waters Corporation (1 mentions) Dad detector, by Agilent Technologies (1 mentions) J 715 spectropolarimeter, by Jasco (1 mentions) 400 mhz spectrometer, by Bruker (1 mentions) 1200 series lc, by Agilent Technologies (1 mentions) Ods a c18 column, by YMC (1 mentions) Nicolet 6700 spectrometer, by Thermo Fisher Scientific (1 mentions) J 815 spectropolarimeter, by Jasco (1 mentions) Rp 18 f254, by Merck Group (1 mentions) Uv 1800 spectrometer, by Shimadzu (1 mentions) 500 mhz spectrometer, by Bruker (1 mentions) Silica gel, by YMC (1 mentions) Pack ods a c18 column, by YMC (1 mentions)

8 protocols using mcp100 polarimeter

Analytical Techniques for Chemical Characterization

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The NMR spectra were recorded on a Bruker 400 MHz spectrometer using TMS as an internal standard. The HRESIMS spectra were measured on a Waters Xevo G2 Q-TOF (Waters) mass spectrometer. Optical rotations were measured with an Anton Paar MCP100 polarimeter. CD spectra were measured on a JASCO J-715 spectropolarimeter. The semipreparative HPLC was carried out on an Agilent technologies 1260 infinity instrument equipped with DAD detector (Agilent, USA) using a reversed-phase C18 column (5 μm, 10 × 250 mm; Cosmosil, Japan). Column chromatography (CC) was performed on silica gel, Sephadex LH-20 (Amersham Pharmacia Biotech AB), and ODS (50 μm, Daiso, Japan).

Xie C.L., Xia J.M., Lin T., Lin Y.J., Lin Y.K., Xia M.L., Chen H.F., Luo Z.H., Shao Z.Z, & Yang X.W. (2019). Andrastone A From the Deep-Sea-Derived Fungus Penicillium allii-sativi Acts as an Inducer of Caspase and RXRα-Dependent Apoptosis. Frontiers in Chemistry, 7, 692.

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Spectroscopic Characterization of Compound

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For IR spectroscopy, the sample was first dissolved in methanol at 0.05 g mL⁻¹. A drop of this solution is deposited on surface of KBr cell. The solution was then evaporated to dryness and the film formed on the cell is analyzed directly on a Nicolet IN 10 Micro FTIR spectrometer by transmission mode (Supplementary Data 2). For UV spectroscopy, the same solution was analyzed by the DAD detector of an Agilent 1200 series LC and scanned from 210 to 600 nm (Supplementary Data 2). For optical rotation, the same solution was analyzed on a Anton Paar MCP100 polarimeter and the specific rotation was [α]²⁵_D = + 7.25 (c = 0.035, CHCl₃). Values represent means ± s.d. of three technical replicates.

Xue Z., Xu X., Zhou Y., Wang X., Zhang Y., Liu D., Zhao B., Duan L, & Qi X. (2018). Deficiency of a triterpene pathway results in humidity-sensitive genic male sterility in rice. Nature Communications, 9, 604.

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Comprehensive Analytical Characterization

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The NMR spectroscopy spectra were obtained on the Bruker ASCEND 600 MHz NMR spectrometer equipped with CryoProbe (Bruker Biospin GmbH, Rheinstetten, Germany). Optical rotations were recorded on an Anton Paar MCP-100 polarimeter (Anton Paar GmbH, Austria), with MeOH as solvent. UV spectra were recorded on a UV-1800 spectrometer (Shimadzu Co., Kyoto, Japan). IR spectra were measured on the Nicolet 6700 spectrometer (Thermo, Madison, WI, USA). CD spectra were measured on a J-815 spectropolarimeter (Jasco Co., Japan). Crystallographic data was collected on an XtaLAB Pro: Kappa single four-circle diffractometer using Cu Kα radiation (Rigaku Co., Tokyo, Japan). HRESIMS spectra data were recorded on a MaXis quadrupole-time-of-flight mass spectrometer (Bruker Biospin GmbH, Rheinstetten, Germany). Normal and reverse phase column chromatography (C. C.) was performed using silica gel (200–300 mesh, Qingdao Haiyang Chemical, Qingdao, China) and ODS (YMC Co., Ltd., Kyoto, Japan), respectively. Normal and reverse phase thin-layer chromatography (TLC) was conducted using silica gel 60 F₂₅₄ and RP-18 F₂₅₄ (Merck Millipore Co., Darmstadt, Germany). HPLC was performed using Shimadzu LC-16P system (Shimadzu Co., Kyoto, Japan) with YMC-ODS-A C₁₈ Column (20 × 250 mm, 5 µm) for separation. Analytical and HPLC grade reagents (Macklin Co., Shanghai, China) were used for isolation procedures.

Li H., Liu X., Li X., Hu Z, & Wang L. (2021). Novel Harziane Diterpenes from Deep-Sea Sediment Fungus Trichoderma sp. SCSIOW21 and Their Potential Anti-Inflammatory Effects. Marine Drugs, 19(12), 689.

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NMR, HRESIMS, and Optical Rotation Analysis

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NMR spectra were recorded on Bruker 500 MHz spectrometer using TMS as an internal standard. The HRESIMS spectra were measured on a Waters Xevo G2 Q-TOF mass spectrometer. Optical rotations were measured with an Anton Paar MCP100 polarimeter. Chemical shifts were recorded in δ values using solvent signals DMSO-d₆ (δ_H 2.50/δ_C 39.5) and CD₃OD (δ_H 3.0/δ_C 49.0) as references. Column chromatography (CC) was performed on silica gel, Sephadex LH-20, and ODS (octadecyl silane).

Yang X., He Z., Zheng Y., Wang N., Mulinge M., Schmit J.C., Steinmetz A, & Seguin-Devaux C. (2021). Chemical Constituents of Cassia abbreviata and Their Anti-HIV-1 Activity. Molecules, 26(9), 2455.

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Spectroscopic Characterization of Compounds

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NMR spectroscopy was performed using a Bruker ASCEND 600 MHz NMR spectrometer equipped with a CryoProbe (Bruker Biospin GmbH, Rheinstetten, Germany). Optical rotations were measured on an Anton Paar MCP-100 polarimeter (Anton Paar GmbH, Graz, Austria) and the HRESIMS spectra were acquired on a MaXis quadrupole-time-of-flight mass spectrometer (Bruker Biospin GmbH, Rheinstetten, Germany). Silica gel (200–300 mesh, Qingdao Haiyang Chemical, Qingdao, China) and YMC Gel ODS-A (150 µM, YMC Co., Ltd., Kyoto, Japan) were used for column chromatography and Silica gel 60 F₂₅₄ and RP-18 F₂₅₄ thin-layer chromatography (TLC) plates (Merck Millipore Co., Darmstadt, Germany) were used for TLC. HPLC was performed using a Shimadzu LC-16P system (Shimadzu Co., Tokyo, Japan) equipped with a YMC-Pack ODS-A C₁₈ column (20 × 250 mm, 5 µm; YMC Co., Ltd., Kyoto, Japan). Analytical- and HPLC-grade reagents (Macklin Co., Shanghai, China) were used for the isolation procedures.

Li H., Liu X., Hu Z, & Wang L. (2022). Novel Sesquiterpene and Diterpene Aminoglycosides from the Deep-Sea-Sediment Fungus Trichoderma sp. SCSIOW21. Marine Drugs, 21(1), 7.

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Characterization of Organic Compounds

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Common solvents were dried and distilled by standard procedures. All starting materials and reagents were obtained commercially and used without further purifications. Elution flash chromatography was conducted on silica gel (230–400 mesh ASTM). Thin layer chromatography (TLC) was performed on silica gel plates (silica gel 60 F254, supported on aluminum). Melting points were measured with a Gallekamp apparatus. NMR spectra were recorded in CDCl₃ on a Brucker DPX 300 MHz spectrometer at 20 °C. Chemical shifts are reported in units of ppm relative to the solvent residue peaks (CDCl₃, δ = 7.260 ppm for ¹H, 77.16 for ¹³C and CD₂Cl₂, δ = 7.320 ppm for ¹H, 54.00 for ¹³C). DEPT-135 NMR experiments were used for the assignation of the type of carbon nucleus (C, CH, CH₂, CH₃). Complex spin-system signals were additionally simulated using MestRe-C (Cobas, C.; Cruces, J.; Sardina, J., MestRe-C program version 2.3). FTIR spectra were recorded with Bruker Alpha-7 spectrometer from neat samples using ATR technique. IR bands are given in cm⁻¹. High-resolution mass spectrometry (HRMS) was performed using the ESI technique for the ionization and ion tramp (positive mode) for the detection. Optical rotations in chloroform solution (dye concentration, c, expressed in g/100 mL) were recorded at 293 K on an Anton Paar MCP 100 polarimeter.

Sola-Llano R., Jiménez J., Avellanal-Zaballa E., Johnson M., Cabreros T.A., Moreno F., Maroto B.L., Muller G., Bañuelos J., Cerdán L., García-Moreno I, & de la Moya S. (2019). BOPHYs versus BODIPYs: A comparison of their performance as effective multi-function organic dyes. Dyes and pigments : an international journal, 170, 107662.

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Comprehensive Analytical Characterization

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Optical rotations were recorded on an Anton Paar MCP 100 polarimeter. ECD spectra were recorded on a Chirascan spectropolarimeter. The HRESIMS spectra were recorded on Q-Exactive Focus tandem mass spectrometry. The NMR spectra were recorded on a Bruker AVANCE III 400 MHz spectrometer. The preparative and semipreparative HPLC were performed on an Agilent Technologies 1260 infinity instrument using ODS or Chiralpak IC columns. UV spectra were recorded on a UV-8000 UV/Vis spectrometer. Column chromatography (CC) was performed on silica gel and Sephadex LH-20. The TLC plates were visualized under UV light or by spraying with 10% H₂SO₄.

Zou Z.B., Zhang G., Zhou Y.Q., Xie C.L., Xie M.M., Xu L., Hao Y.J., Luo L.Z., Zhang X.K., Yang X.W, & Wang J.S. (2022). Chemical Constituents of the Deep-Sea-Derived Penicillium citreonigrum MCCC 3A00169 and Their Antiproliferative Effects. Marine Drugs, 20(12), 736.

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Spectroscopic Characterization of Compounds

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The HR-ESI-MS spectra were obtained on a Waters Xevo G2 Q-TOF mass spectrometer equipped with a Spray™ ESI source in both the positive and negative ion mode. NMR spectra were recorded in CDCl₃, CD₃OD, or DMSO-d₆ on a Bruker Avance III 400 Mz spectrometer at room temperature. Optical rotation was measured by an Anton Paar MCP 100 polarimeter. UV and ECD spectra were acquired on a JASCO J-810 spectropolarimeter. Preparative HPLC (high-performance liquid chromatography) separations for purification were carried out on an Agilent 1260 system with a semi-preparative chromatographic column (COSMOSIL 5C₁₈-MS-II, 5PFP, SL-II, 250 mm × 10 mm). Materials for column chromatography (CC) included silica gel, ODS (octadecylsilyl), and Sephadex LH-20.

Hao Y.J., Zou Z.B., Xie M.M., Zhang Y., Xu L., Yu H.Y., Ma H.B, & Yang X.W. (2023). Ferroptosis Inhibitory Compounds from the Deep-Sea-Derived Fungus Penicillium sp. MCCC 3A00126. Marine Drugs, 21(4), 234.

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