Fitc anti mouse cd3ε

Manufactured by BD

FITC anti-mouse CD3ε is a fluorescent-conjugated antibody that binds to the CD3ε subunit of the T cell receptor complex in mice. It is commonly used in flow cytometry applications to identify and quantify T cells.

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2 protocols using fitc anti mouse cd3ε

Comprehensive Immune Cell Profiling

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APC anti-mouse CD4 (clone: RM4-5), BV605 anti-mouse CD4 (clone: GK1.5), FITC anti-mouse CD3ε, PE anti-mouse CD8α, V500 rat anti-mouse B220, FITC anti-mouse CD45R/B220, APC anti-mouse IgD, APC anti-mouse CD21/CD35, PE anti-mouse CD23, FITC anti-mouse CD8, BV786 anti-mouse CD3, PE CF594 anti-mouse CD23, APC Cy7 anti-mouse CD21, FITC anti-mouse Gr1, APC anti-mouse CD11b, PE anti-mouse F4/80, FITC anti-mouse I-A/E, and APC streptavidin were bought from BD bioscience (San Jose, CA). Biotinylated anti-mouse C3 was obtained from Cedarlane (Burlington, Canada), and APC anti-mouse IgG, APC anti-mouse IgM and FITC anti-mouse IgM were purchased from Jackson Immunoresearch (West Grove, PA). PE anti-mouse CD1d and Zombie yellow live dead was purchased from Biolegend (San diego, CA).

Patel S.R., Gibb D.R., Girard-Pierce K., Zhou X., Rodrigues L.C., Arthur C.M., Bennett A.L., Jajosky R.P., Fuller M., Maier C.L., Zerra P.E., Chonat S., Smith N.H., Tormey C.A., Hendrickson J.E, & Stowell S.R. (2018). Marginal Zone B Cells Induce Alloantibody Formation Following RBC Transfusion. Frontiers in Immunology, 9, 2516.

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Multiparameter Immune Cell Analysis

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The following antibodies were used for NK cell analysis: FITC anti-mouse CD3ε (BD Biosciences, Cat# 553062, clone 145–2 C11), APC anti-mouse NK1.1 (eBioscience/Affymetrix, Cat# 17–5941, clone PK136), PerCp-Cy5.5 anti-mouse CD45 (BioLegend, Cat# 103132, clone 30-F11) and PE anti-mouse CD69 (BioLegend, Cat# 104507, clone H12F3). For pDCs analysis we used: PE-anti-mouse CD317 (PDCA-1) (BioLegend, Cat# 127104, clone 129C1), PerCP/Cy5.5 anti-mouse CD45 (BioLegend, Cat# 103132, clone 30-F11), APC anti-mouse CD11c (BioLegend, Cat# 117310, clone N418) and FITC anti-mouse/human CD45R/B220 (BioLegend, Cat# 103206, clone RA3-6B2). For myeloid cells we used: PerCp-Cy5.5 anti-mouse CD11b (BioLegend, Cat# 101228, clone M1/70), APC anti-mouse CD45 (BioLegend, Cat# 103112, clone 30-F11), FITC-anti-mouse Ly6C/Ly6G (Gr-1) (BioLegend, Cat# 108406, clone RB6-8 C5), PB anti-mouse F4/80 (BioLegend, Cat# 123123, clone BM8). For TLR7 expression analysis, we procured PE-anti-mouse TLR7 (BD Biosciences, Cat# 565557, clone A94B10). Exclusion of dead cells from the analysis was done based on staining with LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit (ThermoFisher Scientific Cat# L34957), or DAPI (4ʹ6’-diamino-phenylindoledihydrochloride, ThermoFisher Scientific, Cat# D21490), or fixable viability dye eFluor780 (ThermoFisher Scientific, Cat# 65–0865-14).

Shah D., Comba A., Faisal S.M., Kadiyala P., Baker G.J., Alghamri M.S., Doherty R., Zamler D., Nuñez G., Castro M.G, & Lowenstein P.R. (2021). A novel miR1983-TLR7-IFNβ circuit licenses NK cells to kill glioma cells, and is under the control of galectin-1. Oncoimmunology, 10(1), 1939601.

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