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Hecd1 anti e cadherin

Manufactured by Takara Bio
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Sourced in Japan

HECD1 is an anti-E-cadherin antibody produced by Takara Bio. E-cadherin is a cell-cell adhesion molecule that plays a critical role in the maintenance of epithelial tissue integrity. The HECD1 antibody can be used to detect E-cadherin expression in various applications.

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Lab products found in correlation

P124 anti dsg1 extracellular domain, by Progen Biotechnik (2 mentions) 27b2 anti dsg1 cytodomain, by Thermo Fisher Scientific (2 mentions) U100 anti dsc1, by Progen Biotechnik (2 mentions) Anti p63 4a4, by Santa Cruz Biotechnology (2 mentions) Anti perk c33e10, by Cell Signaling Technology (2 mentions) Anti erk1 2, by Promega (2 mentions) Gapdh, by Merck Group (2 mentions) Anti melan a, by Abcam (1 mentions) Total erk1 2, by Cell Signaling Technology (1 mentions) Anti p erk, by Cell Signaling Technology (1 mentions) Anti slug, by Cell Signaling Technology (1 mentions) U0126, by Cell Signaling Technology (1 mentions) 4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions) Anti gapdh, by Abcam (1 mentions)

Spelling variants of this product

E-cadherin (10 citations) Anti-E-cadherin (3 citations)

3 protocols using hecd1 anti e cadherin

1

Antibody Characterization for Desmosomal and Epidermal Proteins

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Mouse monoclonal antibodies used: P124 (anti–Dsg1 extracellular domain; Progen, Heidelberg, Germany); 27B2 (anti–Dsg1 cytodomain; Invitrogen), U100 (anti-Dsc1; Progen), HECD1 (anti– E-cadherin; Takara, Kyoto, Japan) and 4A4 (anti-p63; Santa Cruz Biotechnology, Dallas, TX). Rabbit polyclonal antibodies used: K1, K10, and loricrin (gifts from J. Segre, National Human Genome Research Institute, Bethesda, MD), 1905 (anti-Dsg3; gift from J. Stanley, University of Pennsylvania, Philadelphia, PA), C33E10 (anti-pERK; Cell Signaling Technology, Danvers, MA), anti-Erk1/2 (Promega, Madison, WI), H-137 (anti-p63; Santa Cruz Biotechnology) and GAPDH (glyceraldehyde-3-phosphate dehydrogenase; Sigma-Aldrich). Chicken polyclonal antibody used: Pg (1407; Aves Laboratories, Tigard, OR). Secondary antibodies for immunoblotting were goat anti–mouse, –rabbit, and –chicken peroxidase (Rockland; KPL, Gaithersburg, MD). Secondary antibodies for immunofluorescence microscopy were goat anti– mouse, –rabbit, and –chicken linked to fluorophores of 488 nm and 568 nm (Alexa Fluor; Invitrogen).
Johnson J.L., Koetsier J.L., Sirico A., Agidi A.T., Antonini D., Missero C, & Green K.J. (2014). The desmosomal protein Desmoglein 1 aids recovery of epidermal differentiation after acute ultraviolet light exposure. The Journal of investigative dermatology, 134(8), 2154-2162.
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2

Antibody Characterization for Desmosomal and Epidermal Proteins

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Mouse monoclonal antibodies used: P124 (anti–Dsg1 extracellular domain; Progen, Heidelberg, Germany); 27B2 (anti–Dsg1 cytodomain; Invitrogen), U100 (anti-Dsc1; Progen), HECD1 (anti– E-cadherin; Takara, Kyoto, Japan) and 4A4 (anti-p63; Santa Cruz Biotechnology, Dallas, TX). Rabbit polyclonal antibodies used: K1, K10, and loricrin (gifts from J. Segre, National Human Genome Research Institute, Bethesda, MD), 1905 (anti-Dsg3; gift from J. Stanley, University of Pennsylvania, Philadelphia, PA), C33E10 (anti-pERK; Cell Signaling Technology, Danvers, MA), anti-Erk1/2 (Promega, Madison, WI), H-137 (anti-p63; Santa Cruz Biotechnology) and GAPDH (glyceraldehyde-3-phosphate dehydrogenase; Sigma-Aldrich). Chicken polyclonal antibody used: Pg (1407; Aves Laboratories, Tigard, OR). Secondary antibodies for immunoblotting were goat anti–mouse, –rabbit, and –chicken peroxidase (Rockland; KPL, Gaithersburg, MD). Secondary antibodies for immunofluorescence microscopy were goat anti– mouse, –rabbit, and –chicken linked to fluorophores of 488 nm and 568 nm (Alexa Fluor; Invitrogen).
Johnson J.L., Koetsier J.L., Sirico A., Agidi A.T., Antonini D., Missero C, & Green K.J. (2014). The desmosomal protein Desmoglein 1 aids recovery of epidermal differentiation after acute ultraviolet light exposure. The Journal of investigative dermatology, 134(8), 2154-2162.
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3

Monoclonal Antibodies for Immunoblotting and Immunofluorescence

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The mouse monoclonal antibodies used were P124 (anti-Dsg1 extracellular domain; catalog no. 651111; Progen), anti-Dsg3 (5G11; catalog no. MABT335; EMD Millipore), 27B2 (anti-Dsg1 cytodomain; catalog no. 32-6000; Life Technologies), HMB45 (anti-Melanoma gp100; catalog no. MA5-13232; Thermo Fisher Scientific), and total ERK1/2 (catalog no. 4370S; Cell Signaling). The rabbit monoclonal antibody EP1576Y (anti-S100 beta; catalog no. ab52642; Abcam) was used. Rabbit polyclonal antibodies used were HECD1 (anti-E-cadherin; Takara), anti-Melan-A (catalog no. ab15468; Abcam), anti-GAPDH (glyceraldehyde-3-phosphate dehydrogenase; catalog no. G9545; Sigma-Aldrich), anti-GRHL1 (catalog no. PA5-31734; Thermo Fisher Scientific), anti-Slug (catalog no. 9585S; Cell Signaling), and anti-p-ERK (catalog no. 4370S; Cell Signaling). Secondary antibodies for immunoblotting were goat anti-mouse and goat anti-rabbit (LI-COR). Secondary antibodies for immunofluorescence were goat anti-mouse and goat-anti-rabbit linked to fluorophores of 488, 568, and 647 nm (Alexa Fluor; Life Technologies). DAPI (catalog no. 9542; Sigma-Aldrich) was used to stain nuclei. The MEK1/2 inhibitor (U0126) was purchased from Cell Signaling (catalog no. 9903S) and used at a 5 μM final concentration. The CXCR2 inhibitor (SB22502) was purchased from Selleck Chemicals (catalog no. S7651) and used at 500 nM final concentration.
Burks H.E., Pokorny J.L., Koetsier J.L., Roth-Carter Q.R., Arnette C.R., Gerami P., Seykora J.T., Johnson J.L., Ren Z, & Green K.J. (2023). Melanoma cells repress Desmoglein 1 in keratinocytes to promote tumor cell migration. The Journal of Cell Biology, 222(11), e202212031.
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