Tcr allophycocyanin apc clone ip26

Manufactured by Thermo Fisher Scientific

TCR-allophycocyanin (APC; clone IP26) is a fluorescently-labeled antibody that binds to the T-cell receptor (TCR) on the surface of T cells. Allophycocyanin (APC) is the fluorescent dye conjugated to the antibody, which allows for the detection and analysis of TCR-expressing cells using flow cytometry or other fluorescence-based techniques.

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Lab products found in correlation

Cd14 viogreen clone t k4, by Miltenyi Biotec (2 mentions) Efluor 506 ef506, by Thermo Fisher Scientific (2 mentions) Cd3 apc, by BioLegend (1 mentions) Cd27 pe cy7, by BD (1 mentions) Ccr7 pe cy7, by BioLegend (1 mentions) Cd8 brilliant violet 785, by BioLegend (1 mentions)

2 protocols using tcr allophycocyanin apc clone ip26

Multiparameter Flow Cytometry Staining

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The following antibodies were used for cell surface staining: CD3-eFluor 780-allophycocyanin (eF780-APC; clone UCHT1), TCR-allophycocyanin (APC; clone IP26), and IL-2–APC (clone MQ1-17H12) (all from eBioscience); CD4-BD Horizon R phycoerythrin-CF594 (PE-CF594; clone RPA-T4), CD27-PE-Cy7 (Pe-Cy7-clone M-T271), CXCR5-Alexa Fluor 488 (AF488; clone RF8B2), and CCR7-Alexa Fluor 647 (AF647; clone 3D12) (all from BD Biosciences); CD14-Viogreen (clone TÜK4) and CD20-Viogreen (clone LT29) (from Miltenyi Biotec); and CD38-Alexa Fluor 700 (AF700; clone HIT2), CD20-peridinin chlorophyll protein (PerCP)/Cy5.5 (clone 2H7), CD8-brilliant violet 785 (BV785; clone RPA-T8), CD45RA-brilliant violet 421 (BV421; clone HI100), CCR7-PE-Cy7 (clone G043H7), and CD3-APC (clone SK7) (all from BioLegend). The fixable viability dye eFluor 506 (eF506; eBioscience) was added to restrict the analysis to live cells.

Claireaux M., Galperin M., Benati D., Nouël A., Mukhopadhyay M., Klingler J., de Truchis P., Zucman D., Hendou S., Boufassa F., Moog C., Lambotte O, & Chakrabarti L.A. (2018). A High Frequency of HIV-Specific Circulating Follicular Helper T Cells Is Associated with Preserved Memory B Cell Responses in HIV Controllers. mBio, 9(3), e00317-18.

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Comprehensive Immune Phenotyping Protocol

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The following antibodies were used for cell surface staining: CD3-eFluor 780-Allophycocyanin (eF780-APC, clone UCHT1), TCR-Allophycocyanin (APC, clone IP26) (all from eBioscience); CD4 BD Horizon R-phycoerythrin-CF594 (PE-CF594, clone RPA-T4), CD4-R-phycoerythrin-cyanine 7 (PE-Cy7, clone SK3), CD69-R-phycoerythrin (PE, clone FN50), (all from BD Biosciences); CD14-Viogreen (clone TÜK4), and CD19-VioGreen (clone LT19) (from Miltenyi Biotec); TRBV2-PE (clone IMMU 546, Beckman Coulter); CD8-Brilliant Violet 785 (BV785, clone RPA-T8), HLA-DR-PE-Cy7 (clone LN3), CD45RA-Brilliant Violet 421 (BV421, clone HI100), CCR7-PE-Cy7 (clone G043H7) (all from Biolegend). The fixable viability dye eFluor 506 (eF506, eBioscience) was added to gate out dead cells.

Mukhopadhyay M., Galperin M., Patgaonkar M., Vasan S., Ho D.D., Nouël A., Claireaux M., Benati D., Lambotte O., Huang Y, & Chakrabarti L.A. (2017). DNA vaccination by electroporation amplifies broadly cross-restricted public TCR clonotypes shared with HIV controllers. Journal of immunology (Baltimore, Md. : 1950), 199(10), 3437-3452.

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