Fluorescent Live Cell/Dead Cell Assay. Cells were treated and plated as above for MitoSox endpoint assays, incubated with Multiplex Fluorometric Cytotoxicity Assay reagent (Promega) for 45 min, then assessed with λex = 400nm/λem = 505 nm for live cells, and λex = 485nm/λem = 520 nm for dead cells.
Lactate Dehydrogenase (LDH) Release Assay. Homogenous Membrane Integrity Assay (Promega) was used to measure LDH released into the media by 5 × 105 cells per well after 18 h incubation with LPS, with Cyto-Tox One fluorescence measured at λex = 560nm/λem = 590 nm.
Lactate Dehydrogenase (LDH) Release Assay. Homogenous Membrane Integrity Assay (Promega) was used to measure LDH released into the media by 5 × 105 cells per well after 18 h incubation with LPS, with Cyto-Tox One fluorescence measured at λex = 560nm/λem = 590 nm.