Prl tk vector expressing renilla luciferase
The PRL-TK vector is a plasmid construct that expresses the Renilla luciferase reporter gene. The Renilla luciferase gene is driven by the PRL (Rous Sarcoma Virus Long Terminal Repeat) promoter and the thymidine kinase (TK) polyadenylation signal. This vector can be used for various applications that require the expression of the Renilla luciferase reporter.
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6 protocols using prl tk vector expressing renilla luciferase
Measuring BCL-2 Promoter Activity in Glioblastoma Cells
Wnt/β-catenin Pathway Activation by G. lucidum
103 cells/well) and incubated for 24 hours, then transfected with
200 ng TOP-Flash/FOP-Flash (Millipore, Burlington, MA), and 20 ng pRL-TK vector
expressing Renilla luciferase (Promega, Madison, WI), following the recommended
protocol using Lipofectamine 3000 (Thermo Scientific), for another 24 hours.
Subsequently, cells were divided into 2 groups: G lucidum (0,
100, or 200 µg/mL for 24 hours) or CHIR-99021 (inhibitor of GSK-3α/β, Selleck,
Houston, TX) + G lucidum (cells were pretreated with 10 µm
CHIR-99021 for 24 hours to activate the Wnt/β-catenin signaling pathway), and OD
values of the TOP flash and FOP flash were detected by a dual-luciferase
reporter assay system (Promega) from cell lysates. Activity of the Wnt/β-catenin
signaling pathway was reflected by the TOP/FOP ratio.
Validating miR-539 Target Gene DLX1
Modulating Wnt/β-catenin Signaling
Bdnf Promoter-Driven Luciferase Assay
HCMV UL54 Promoter Analysis
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