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Anti ha antibody clone c29f4

Manufactured by Cell Signaling Technology
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The Anti-HA antibody (clone: C29F4) is a primary antibody that recognizes the HA (Hemagglutinin) tag, a commonly used epitope tag for protein expression and detection. The antibody is designed for immunological applications such as Western blotting, immunoprecipitation, and immunofluorescence.

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Close spelling variants of this product

Rabbit anti-HA antibody (clone C29F4) Anti-HA (clone C29F4, Anti-HA antibody C29F4 HA (clone C29F4), Anti-HA (C29F4) HA (C29F4) Clone C29F4 Anti-HA antibody Anti-HA

2 protocols using anti ha antibody clone c29f4

1

Lentiviral Transduction of Murine Cancer Cell Lines

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LLC, 4T1, B16-F10 melanoma and MutuDC1940 cells were transduced with HA tagged versikine (Vkine) - or empty vector (EV)- containing lentivirus as detailed above. The cells were selected with 10μg/mL blasticidin for 2 weeks. HA-tagged versikine expression was confirmed by western blotting using anti-HA antibody (clone: C29F4, Cell Signaling). LLC- EV or -Vkine cell lines were transduced with pHIV-Luc-OVA-ZsGreen lentivirus. LLC-OVA expressing cells were FACS-sorted based on ZsGreen expression to ensure comparable transduction rates between different cell lines.
Papadas A., Deb G., Cicala A., Officer A., Hope C., Pagenkopf A., Flietner E., Morrow Z.T., Emmerich P., Wiesner J., Arauz G., Bansal V., Esbona K., Capitini C.M., Matkowskyj K.A., Deming D.A., Politi K., Abrams S.I., Harismendy O, & Asimakopoulos F. (2022). Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment. Cell reports, 40(7), 111201.
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2

Lentiviral Transduction of Murine Cancer Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
LLC, 4T1, B16-F10 melanoma and MutuDC1940 cells were transduced with HA tagged versikine (Vkine) - or empty vector (EV)- containing lentivirus as detailed above. The cells were selected with 10μg/mL blasticidin for 2 weeks. HA-tagged versikine expression was confirmed by western blotting using anti-HA antibody (clone: C29F4, Cell Signaling). LLC- EV or -Vkine cell lines were transduced with pHIV-Luc-OVA-ZsGreen lentivirus. LLC-OVA expressing cells were FACS-sorted based on ZsGreen expression to ensure comparable transduction rates between different cell lines.
Papadas A., Deb G., Cicala A., Officer A., Hope C., Pagenkopf A., Flietner E., Morrow Z.T., Emmerich P., Wiesner J., Arauz G., Bansal V., Esbona K., Capitini C.M., Matkowskyj K.A., Deming D.A., Politi K., Abrams S.I., Harismendy O, & Asimakopoulos F. (2022). Stromal remodeling regulates dendritic cell abundance and activity in the tumor microenvironment. Cell reports, 40(7), 111201.
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