Spectramax 190 detection microplate reader

HT22 cells were plated on 96-well plates and cultured with or without 1 μM Fer-1 (Selleck Chemicals, Houston, TX, USA) for 18 h before EMP exposure. Cell viability was evaluated by using a Cell Counting Kit-8 (CCK-8) Colorimetric Assay (CK04, Dojindo, Kumamoto, Japan) according to the manufacturer’s instructions. The average optical densities (ODs) of the plates were measured using a SpectraMax 190-detection microplate reader (Molecular Devices, San Jose, CA, USA) at a wavelength of 450 nm. The average percentage of neuronal viability in each group was calculated by comparison to the sham group.

After the EMP exposure, the levels of lipid peroxidation in rat hippocampal tissues were assessed by using a Lipid Peroxidation malonaldehyde (MDA) Assay Kit (MAK085, Sigma-Aldrich, St. Louis, MO, USA) according to the manufacturer’s instructions. The average ODs of the samples were measured using a SpectraMax 190-detection microplate reader (Molecular Devices, San Jose, CA, USA) at a wavelength of 532 nm.
HT22 cells were seeded in 6-well plates and cultured with or without 1 μM Fer-1 (Selleck Chemicals, Houston, TX, USA) for 18 h before EMP exposure. After the EMP exposure, the levels of lipid peroxidation in HT22 cells were measured using the same protocol as that implemented in the corresponding hippocampus experiments.