Hydroxyethyl methacrylate, TEA and boron trifluoride diethyl etherate were purchased from MACKLIN (Shanghai, China). Doxorubicin and beta-D-galactose pentaacetate were obtained from Energy Chemical (Shanghai, China). (Hydroxymethyl) phenylboronic acid pinacol ester, 2,2′-azobis(2-methylpropionitrile) (AIBN, 99%), methacryloyl chloride and 2-(dodecylthiocarbonothioylthio)-2-methylpropionic acid were purchased from Aladdin (Shanghai, China). Dialysis bag MD34 (3500 Da) was obtained from Solarbio (Beijing, China). DiR were purchase from MeilunBio (Suzhou, China). MTT solution, 2% phosphotungstic acid and 4′,6-diamidino- 2-phenylindole (DAPI) were obtained from Solaibio (Shanghai, China).
Female mouse 4T1 cells and human breast cancer MDA-MB-231 cells were obtained from American Type Culture Collection (ATCC, USA) and cultured in complete RMPI 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. HUVEC were obtained from ATCC and maintained in HUVEC special culture medium (Procell, Wuhan, China) supplemented with 10% FBS and 1% penicillin/streptomycin. The cells were passaged by mechanical dissociation every two days and further cultured in a humidified incubator with 5% CO2 at 37°C.
Female mouse 4T1 cells and human breast cancer MDA-MB-231 cells were obtained from American Type Culture Collection (ATCC, USA) and cultured in complete RMPI 1640 medium with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin. HUVEC were obtained from ATCC and maintained in HUVEC special culture medium (Procell, Wuhan, China) supplemented with 10% FBS and 1% penicillin/streptomycin. The cells were passaged by mechanical dissociation every two days and further cultured in a humidified incubator with 5% CO2 at 37°C.