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2 protocols using volumetric flask

1

Colorimetric Assay for Heavy Metal Detection

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UV–Vis spectrophotometry (Thermo Scientific, Waltham, MA, USA), pH meter (Eutech, Breda, The Netherlands), oven (Memmert, Schwabach, Germany) micropipette (DLAB, Beijing, China), beaker (Iwaki, Japan), Erlenmeyer (Iwaki, Japan), test tube (Iwaki, Japan), volumetric flask (Iwaki, Japan), stir bar, vacuum filter, volume pipette (Pyrex, Shizuoka, Japan), analytical balance (Sartorius, Göttingen, Germany), ultrasonic stirrer (Branson, MO, USA), Whatman No. 1, Whatman No. 41, Whatman No. 91, Whatman grade 1 chromatography paper, 0.45 µm membrane, and ImageJ software (National Institutes of Health, Bethesda, MD, USA).
AuNPs (10 nm diameter, OD 1) (Sigma Aldrich, St. Louis, MO, USA), cyanuric acid (Sigma Aldrich), ethanol (Merck, Darmstadt, Germany), double distilled H2O (Ikapharmindo Putramas, East Jakarta, Indonesia), HgCl2 (Smartlab, Semarang, Indonesia), MgSO4, ZnCl2, AgNO3, FeSO4, CdSO4, BaCl2, and MnCl2 (Merck, Germany), NaCl (Merck, Germany), Tris-HCl (Himedia, Kennett Square, PA, USA), as well as water samples from Kenanga Lake, FMIPA Lake, and Mahoni Lake.
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2

Passion Fruit Peel Bioactive Extraction

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The materials used in this research were purple passion fruit peel, red passion fruit peel, yellow passion fruit peel, ethanol (E-Merck), ethylene diamine tetraacetic acid (E-Merck), alanine transaminase activity assay kit (Sigma Aldrich), aspartate transaminase activity assay kit (Sigma Aldrich), urea assay kit (Sigma Aldrich), creatinine assay kit (Sigma Aldrich), sodium carboxymethyl cellulose (Tokyo Chemical Industry), and demineralized water (Brataco).
The tools used in this research were cutter (Kenko), drying cabinet (Alumex), blender (Miyako), balance (Mettler Toledo), analytical balance (Mettler Toledo), filter paper (Whatman), dropper (Iwaki), evaporating dish (Iwaki), maceration vessel (Iwaki), beaker glass (Iwaki), measuring glass (Iwaki), volumetric flask (Iwaki), test tube (Iwaki), hot plate stirrer (Thermo), water bath (Memmert), incubator (Memmert), rotary evaporator (Buchi), thermometer (Lutron), test sieve 10 (Retsch), stopwatch (Casio), spectrophotometer (Agilent).
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