Dna og 575 kit

Manufactured by DNA Genotek

Sourced in Canada

The DNA OG-575 kit is a laboratory equipment designed for the collection, stabilization, and transportation of DNA samples. It provides a simple and reliable method for obtaining high-quality DNA from various biological sources.

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Lab products found in correlation

Infinium methylation epic, by Illumina (1 mentions)

2 protocols using dna og 575 kit

Perinatal Influences on DNA Methylation

Cited 1 time

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Saliva is sampled using the DNA OG-575 kit (DNAGenotek, Ottawa, Ontario, Canada). DNA extraction is performed using published methods,36 (link) and DNA methylation (DNAm) analyses are carried out at the Genetics Core of the Edinburgh Clinical Research Facility (Edinburgh, UK) using Illumina Infinium MethylationEPIC (San Diego, California, USA), with interrogation of the arrays against ~850 k methylation sites. We will investigate perinatal influences on DNAm using principal component analysis, mediation and correlation analyses.

Boardman J.P., Hall J., Thrippleton M.J., Reynolds R.M., Bogaert D., Davidson D.J., Schwarze J., Drake A.J., Chandran S., Bastin M.E, & Fletcher-Watson S. (2020). Impact of preterm birth on brain development and long-term outcome: protocol for a cohort study in Scotland. BMJ Open, 10(3), e035854.

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DNA Extraction from Saliva Samples

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The DNA OG-575 kit was used for sampling of saliva at TEA, defined as 38–42 weeks PMA (DNAGenotek, Ottawa, ON, Canada). DNA was extracted using an alcohol precipitation technique as per manufacturer's instructions, and was rehydrated in TE 0.5. Gel electrophoresis was used to qualify DNA extraction and Qubit 2.0 Fluorometer was utilised for quantification of DNA concentration (Invitrogen Life Sciences, Carlsbad, CA, USA).

Sparrow S., Manning J.R., Cartier J., Anblagan D., Bastin M.E., Piyasena C., Pataky R., Moore E.J., Semple S.I., Wilkinson A.G., Evans M., Drake A.J, & Boardman J.P. (2016). Epigenomic profiling of preterm infants reveals DNA methylation differences at sites associated with neural function. Translational Psychiatry, 6(1), e716-.

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