Cells were lysed in cell lysis buffer (50 mM Tris-HCl pH 7.5, 1 mM EDTA, 1 mM EGTA, 1% Triton X-100, 100 mM KCl, 50 mM NaF, 1 mM Na3VO4, 100 nM aprotinin, 1 µM bestatin, 1 mM p-amidinophenylmethanesulfonyl fluoride (PMSF)). Protein lysates (30 μg) were separated by SDS-PAGE and transferred onto PVDF membrane (Bio-Rad, Hercules, CA, USA). Membranes were immunoblotted with the respective antibodies as follows: anti-ROR1 (1:1000), anti-pAKT (1:1000), anti-FGFR1 (1:1000), β-ACTIN (1:5000), anti-AKT (1:1000), anti-EGFR (1:1000) from Cell Signaling (Cell Signaling Technologies, Danvers, MA, USA) and CAV-1 (1:1000) from Santa Cruz (Santa Cruz Biotechnologies, Dallas, TX, USA). Immunoreactive bands were detected using a chemiluminescence based detection system (Bio-Rad).
Anti ror1
Manufactured by Cell Signaling Technology
Sourced in United States
Anti-ROR1 is a laboratory reagent that can be used to detect and study the ROR1 protein in cells and tissues. ROR1 is a receptor tyrosine kinase that plays a role in various cellular processes. The Anti-ROR1 product is designed to enable researchers to investigate the expression and function of ROR1 in their research.
Automatically generated - may contain errors
Lab products found in correlation
Anti akt, by Cell Signaling Technology (1 mentions)
Anti p akt, by Cell Signaling Technology (1 mentions)
Cav 1, by Santa Cruz Biotechnology (1 mentions)
Anti fgfr1, by Cell Signaling Technology (1 mentions)
Pvdf membrane, by Bio-Rad (1 mentions)
Anti egfr, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Anti ask1, by Cell Signaling Technology (1 mentions)
Anti α tubulin, by Merck Group (1 mentions)
Anti c myc, by Santa Cruz Biotechnology (1 mentions)
2 protocols using anti ror1
1
Protein Expression Analysis Protocol
2
Recombinant Protein Immunoblotting Analysis
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Recombinant MKK6 was obtained from Millipore (Watford, UK). Anti‐ROR1, anti‐ASK1, anti‐phospho‐ASK1 (T845), anti‐phospho‐ASK1 (S83), anti‐MKK3/6, anti‐phospho‐MKK3/6 (S189/S207), anti‐p38, anti‐phospho‐p38 (T180/Y182), anti‐mouse IgG, and anti‐rabbit IgG were purchased from Cell Signaling Technology (Danvers, MA, USA). Anti‐c‐myc came from Santa Cruz Biotechnology (Santa Cruz, CA, USA), anti‐GST and anti‐maltose‐binding protein (MBP) from MBL (Nagoya, Japan), Anti‐ROR1 and anti‐goat IgG from R&D Systems (Minneapolis, MN, USA), anti‐JNK and anti‐phospho‐JNK (T183/Y185) from BD Biosciences (Bedford, MA, USA), and anti‐α‐tubulin from Sigma (St. Louis, MO, USA).
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