Nexera x2 lc system

Manufactured by Shimadzu

Sourced in Japan

The Nexera X2 LC system is a high-performance liquid chromatography (HPLC) instrument designed for a wide range of analytical applications. It features a dual-pump configuration, advanced electronics, and automated sample handling capabilities to deliver accurate and reliable results.

Automatically generated - may contain errors

Lab products found in correlation

Analyst version 1, by AB Sciex (2 mentions) Qtrap 5500 system, by AB Sciex (2 mentions) Lcms 8040 liquid chromatograph mass spectrometer, by Shimadzu (1 mentions) Oasis max μelution plate, by Waters Corporation (1 mentions) Prominence lc system, by Shimadzu (1 mentions) Labsolution software, by Shimadzu (1 mentions) Lc 30ad pump, by Waters Corporation (1 mentions) Multiquant software, by AB Sciex (1 mentions) Triple tof 5600 system, by AB Sciex (1 mentions) Chem elut s, by Agilent Technologies (1 mentions) Qtrap 6500 triple quadrupole mass spectrometer, by AB Sciex (1 mentions) Triple quadrupole lcms 8050, by Shimadzu (1 mentions)

Close spelling variants of this product

Nexera X2 LC-30AD HPLC system (7 citations) Nexera X2 LC‐30 UPLC system (2 citations) Nexera X2 UHPLC LC-30A system (2 citations) Nexera LC-30 AD Nexera X2 UHPLC system (2 citations) Nexera X2 system (47 citations) Nexera LC system (15 citations) Nexera X2 (174 citations) Nexera system (25 citations) X2 system (8 citations)

12 protocols using nexera x2 lc system

Quantifying Plasma CP-I and CMPF

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma CP‐I and CMPF concentrations were measured simultaneously according to the methods reported by Suzuki et al.
¹⁷ (link) Briefly, Oasis MAX μElution Plate (Waters) was used to pretreat 250 μL of plasma by solid phase extraction. The extract was analyzed by ultra‐high‐performance liquid chromatography coupled to tandem mass spectrometry using the Nexera X₂ LC system coupled to LCMS−8040 Liquid Chromatograph Mass Spectrometer (Shimadzu) equipped with electrospray ionization. The ¹⁵N₄‐CP‐I and CMPF‐d₅ were used as internal standards for CP‐I and CMPF, respectively. CP‐I was measured with (M + H)⁺ signal in positive ion mode, and CMPF was measured with (M – H)⁻ signal in negative ion mode. The tandem mass spectrometry transitions monitored were mass‐to‐charge ratio (m/z) 655.4 → m/z 596.3 for CP‐I, m/z 659.3 → m/z 600.3 for ¹⁵N₄‐CP‐I, m/z 239.0 → m/z 195.2 for CMPF, and m/z 244.2 → m/z 200.2 for CMPF‐d₅. The assays were validated in accordance with the US Food and Drug Administration Guidance for Bioanalytical Method Validation.
²⁰ The lower limit of quantification was 0.1 ng/mL for CP‐I and 50 ng/mL for CMPF. The within‐batch accuracy of the assay ranged from 92.1% to 110.2% for CP‐I, and from 99.1% to 109.3% for CMPF. The within‐batch precision was less than 7.6% for CP‐I and 3.4% for CMPF.

Ono H., Tanaka R., Suzuki Y., Oda A., Sato H., Tatsuta R., Ando T., Shin T., Ohno K, & Itoh H. (2024). Relationship of plasma 3‐carboxy‐4‐methyl‐5‐propyl‐2‐furanpropanoic acid concentration with OATP1B activity in patients with chronic kidney disease. Clinical and Translational Science, 17(2), e13731.

+ Open protocol

+ Expand

LCMS Sample Preparation for Brain Lipids

Check if the same lab product or an alternative is used in the 5 most similar protocols

For LCMS sample preparation, 10 mg of brain powder prepared from whole‐brain–powered homogenates was mixed with 400 ml of methanol spiked with internal standards and homogenized with a 3 mm tungsten carbide bead (shaken at 25 Hz for 30 s). The methanol fraction was then isolated via centrifugation (20 min at 4°C, 14,000 g, followed by transfer of supernatant to a 96‐well plate, and 1 h incubation at −20°C followed by an additional 20 min centrifugation (4,000 g at 4°C)) and transferred to glass vials for LCMS analysis. For analysis of a GlcCer/GalCer panel, an aliquot of the methanol fraction was dried under N₂ gas and then resuspended in 100 ml of 92.5/5/2.5 CAN/IPA/H2 (MS grade) with 5 mM ammonium formate (MS grade) and 0.5% formic acid (MS grade).
Unless otherwise noted, relative quantification of lipids and metabolites was performed using the Shimadzu Nexera X2 LC system (Shimadzu Scientific Instrument) coupled to Sciex QTRAP 6500+ mass spectrometer (Sciex).

Reifschneider A., Robinson S., van Lengerich B., Gnörich J., Logan T., Heindl S., Vogt M.A., Weidinger E., Riedl L., Wind K., Zatcepin A., Pesämaa I., Haberl S., Nuscher B., Kleinberger G., Klimmt J., Götzl J.K., Liesz A., Bürger K., Brendel M., Levin J., Diehl‐Schmid J., Suh J., Di Paolo G., Lewcock J.W., Monroe K.M., Paquet D., Capell A, & Haass C. (2022). Loss of TREM2 rescues hyperactivation of microglia, but not lysosomal deficits and neurotoxicity in models of progranulin deficiency. The EMBO Journal, 41(4), e109108.

+ Open protocol

+ Expand

High-Resolution Mass Spectrometry Proteome Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Samples were digested and reduced as previously described following recovery by affinity capture. High resolution mass spectrometry was performed on an Orbitrap Hfx attached to a Shimadzu Nexera X2 LC system. Column temperature was set to 40°C. Flow rate was set to 300μL/min. The aqueous solvent (A) was 0.1% (v/v) formic acid in water, and the organic phase (B) was 0.1% (v/v) formic acid in 100% methanol. The gradient started at 5% B and was held for 2 min before being increased to 90% B over 80 min. It was held at 90% B for 4.0 min before returning to 5% B over 2 min, where the column was allowed to re-equilibrate for 1 min. Overall run time was 88 min. The Orbitrap Hfx was operated using a top-10 DDA method. MS scans were performed across a range from 100-1500 m/z at a resolution of 60000. The maximum ion accumulation time was set to 100ms with an AGC target of 3e6. MS/MS scans were performed at a resolution of 30000 with a maximum ion accumulation time of 100ms and an AGC target of 1e5. Nominal collision energy was set to 32 volts. Dynamic exclusion was set to 3 sec using a 4 m/z isolation window. Spray voltage was 3000 volts. Capillary temperature was 360°C. Sheath gas and auxiliary gas were set to 50 and 20 units respectively. The data was searched against the Uniprot database and a Bungarus specific database from NCBI using proteinpilot software.

Timms M., Ganio K, & Steel R. (2020). Extraction of α-neurotoxins from equine plasma by receptor based affinity purification. Drug testing and analysis, 12(7).

+ Open protocol

+ Expand

Quantification of Bile Acids and Bilirubin in Plasma

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total and D‐BIL were quantified as reported previously by enzymatic methods using Iatro LQ T‐BIL and Iatro LQ D‐BIL (LSI Medience, Tokyo, Japan), respectively, according to the manufacturer's protocols, as describe previously.16Other compounds, except for GCDCA‐S, GDCA‐S, GCDCA‐G and GDCA‐G, in the plasma samples were analyzed using LC‐MS/MS. Plasma samples were prepared by protein precipitation as described previously.17 Chromatography was performed on a Prominence LC system, or Nexera X2 LC system (Shimadzu, Kyoto, Japan). Liquid chromatographic conditions were summarized in Supplemental Methods. Note that we modified chromatographic conditions for the bile acids16, 17 to separate the stereoisomers. Data were collected on an AB Sciex API5500 (QTRAP) mass spectrometer (Foster City, CA) operated in electrospray ionization mode. The multiple reaction monitoring precursor/product ion transitions are summarized in Supplemental Methods.

Mori D., Kimoto E., Rago B., Kondo Y., King‐Ahmad A., Ramanathan R., Wood L.S., Johnson J.G., Le V.H., Vourvahis M., David Rodrigues A., Muto C., Furihata K., Sugiyama Y, & Kusuhara H. (2020). Dose‐Dependent Inhibition of OATP1B by Rifampicin in Healthy Volunteers: Comprehensive Evaluation of Candidate Biomarkers and OATP1B Probe Drugs. Clinical Pharmacology and Therapeutics, 107(4), 1004-1013.

+ Open protocol

+ Expand

UPLC-MS/MS Quantitative Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chromatographic analysis was performed on a Shimadzu Nexera X2 ® LC system coupled with a LC-8050 ® tandem mass spectrometer (Shimadzu, Kyoto, Japan). Chromatographic separation was performed on an Acquity UPLC BEH C18 column, m; Waters, Milan, Italy) maintained at 50°C through the column oven.
Compounds separation was obtained through a gradient (Table 1) of mobile phases A (Ammonium acetate 5mM buffer, ph 9,5) and B (ACN) at flow rate of 0.4 mL/min and a time run of 5 minutes.
Auto-sampler was settled at 4°C and the injection volume was 0.3 µL, with a sampling rate of 1 Data processing and system control was managed through the LabSolution ® software (Shimadzu, Kyoto, Japan) version 1.0.

Ariaudo A., Favata F., De Nicolò A., Simiele M., Paglietti L., Boglione L., Cardellino C.S., Carcieri C., Di Perri G, & D'Avolio A. (2016). A UHPLC-MS/MS method for the quantification of direct antiviral agents simeprevir, daclatasvir, ledipasvir, sofosbuvir/GS-331007, dasabuvir, ombitasvir and paritaprevir, together with ritonavir, in human plasma. Journal of pharmaceutical and biomedical analysis, 125.

+ Open protocol

+ Expand

HPLC Analysis of Pharmaceutical Degradation

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Shimadzu Nexera X2 LC system (Shimadzu Corporation, Kyoto, Japan) equipped with a photodiode array detector was used for HPLC analysis. An ACQUITY Ultra Performance LC (UPLC) BEH C8 column (2.1 mm i.d. × 75 mm, 1.7 µm particle size, Waters Corporation, Milford, MA, U.S.A.) was used as the analytical column. ) based on a previous report. 21) (link) The degradation rates were determined from the slopes of the degradation amount versus time plots for each condition. Experimental Ln k data were fit with a linear combination of temperature (-1/T), %RH, and % (w/w) MgSt variables to give the calculated formula using multiple regression with standard least squares fitting and effect leverage.

Tamura K., Ono M., Kawabe T, & Yonemochi E. (2020). Impact of Magnesium Stearate Content: Modeling of Drug Degradation Using a Modified Arrhenius Equation. Chemical & pharmaceutical bulletin, 68(11).

+ Open protocol

+ Expand

Purity Analysis of LNnT by LC-MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 12

For LC-MS analysis to determine purity of LNnT with respect to the dry mass a Nexera X2 LC system (Shimadzu, Kyoto, Japan) was used, which included a LC-30AD pump, an autosampler SIL-30AC, a LCMS-8050 mass spectrometer (ESI-MS detector), a xBridge BEH Amide 3.5 μm, 50×2.1 mm column (Waters, Eschborn, Germany) and a xBridge BEH Amide 3.5 μm pre-column (Waters, Eschborn, Germany). The solvent contained 60% acetonitrile, 39.9% ddH₂O and 0.1% NH₄OH and the flow rate was set to 0.3 mL/min. All analyses were performed with the solvent isocratically at 35° C. Lacto-N-neotetraose was purchased from OligoTech (Crolles, France) and used as internal standard.

US11685758B2. Simple method for the purification of lacto-N-neotetraose (LNnT) from carbohydrates obtained by microbial fermentation (2023-06-27). Chr. Hansen HMO GmbH [DE]. Inventors: Stefan Jennewein [DE], Markus Helfrich [DE].

+ Open protocol

+ Expand

Quantification of Creatinine and 1-NMN

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma and urine samples analyzed by liquid chromatography with tandem mass spectrometry were prepared by protein precipitation as described previously.²⁷ All compounds were separated and detected using the QTRAP5500 system (AB SCIEX, Toronto, Canada) equipped with the Nexera X2 LC system (Shimadzu, Kyoto, Japan), operated in electrospray ionization mode. Analyte quantification was performed using Analyst version 1.7 (Sciex, Toronto, ON, Canada). Creatinine and 1‐NMN were analyzed using creatinine‐d3 and 1‐NMN‐d3 as internal standards, respectively. The conditions of liquid chromatography, multiple reaction monitoring precursor/product ion transitions are summarized in Supplementary Methods.

Miyake T., Kimoto E., Luo L., Mathialagan S., Horlbogen L.M., Ramanathan R., Wood L.S., Johnson J.G., Le V.H., Vourvahis M., Rodrigues A.D., Muto C., Furihata K., Sugiyama Y, & Kusuhara H. (2020). Identification of Appropriate Endogenous Biomarker for Risk Assessment of Multidrug and Toxin Extrusion Protein‐Mediated Drug‐Drug Interactions in Healthy Volunteers. Clinical Pharmacology and Therapeutics, 109(2), 507-516.

+ Open protocol

+ Expand

Targeted Metabolite Quantification in Serum

Check if the same lab product or an alternative is used in the 5 most similar protocols

For quantitative analyses, serum samples were mixed with L-glutamine-¹³C₅, [Tyr]-human FPA, uric acid-1,3–¹⁵N₂, LPC 16:0-d31, and 4PY (internal standards). Varying amounts of standards were spiked into bovine serum albumin solutions in the presence of the internal standard, and extracted. MS analyses were performed using a Triple TOF 5600+ system (SCIEX) fitted with an analyst data acquisition module. The positive ion multiple reaction monitoring (MRM) mode was used for quantitative analysis of L-glutamine, LPC 16:0, LPC 18:0, 2PY, and FPA. Sample extracts (5 μL) were loaded via a Nexera X2 LC system (Shimadzu) onto a three-step linear gradient (solvent A, 0.1% [v/v] FA in water; solvent B, 100% ACN; 1% solvent B for 1.5 min, 1–25% B for 4.5 min, 25–45% B for 2 min, 45–90% B for 2 min, 90% B for 4 min, 90–1% B for 0.5 min, and 1% B for 5.5 min). The negative ion MRM mode was used for quantitative analysis of uric acid levels. LC separations (10 min/sample) were performed using a one-step linear gradient (solvent A, 0.1% [v/v] FA in water; solvent B, 100% ACN; 1% solvent B for 1.5 min, 1–90% B for 2.5 min, 90% B for 2 min, 90–1% B for 0.5 min, and 1% B for 3.5 min). The six metabolites were quantified with the aid of MultiQuant software (SCIEX).

Kim K.H., Joo J., Park B., Park S.J., Lee W.J., Han S.S., Kim T.H., Hong E.K., Woo S.M, & Yoo B.C. (2017). Reduced levels of N’-methyl-2-pyridone-5-carboxamide and lysophosphatidylcholine 16:0 in the serum of patients with intrahepatic cholangiocarcinoma, and the correlation with recurrence-free survival. Oncotarget, 8(68), 112598-112609.

+ Open protocol

+ Expand

LC-MS/MS Quantification of Biological Analytes

Check if the same lab product or an alternative is used in the 5 most similar protocols

Plasma and urine samples analyzed by liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) were prepared by protein precipitation, as described previously¹⁴ with minor modification. All compounds were separated and detected using the QTRAP5500 system (AB SCIEX, Toronto, Canada) equipped with a Nexera X2 LC system (Shimadzu, Kyoto, Japan), operating in electrospray ionization mode. Analyte quantification was performed using Analyst version 1.7 (Sciex, Toronto, Canada). The conditions of liquid chromatography, multiple reaction monitoring precursor/product ion transitions are summarized in Supplementary Methods.

Mochizuki T., Zamek‐Gliszczynski M.J., Yoshida K., Mao J., Taskar K., Hirabayashi H., Chu X., Lai Y., Takashima T., Rockich K., Yamaura Y., Fujiwara K., Mizuno T., Maeda K., Furihata K., Sugiyama Y, & Kusuhara H. (2022). Effect of Cyclosporin A and Impact of Dose Staggering on OATP1B1/1B3 Endogenous Substrates and Drug Probes for Assessing Clinical Drug Interactions. Clinical Pharmacology and Therapeutics, 111(6), 1315-1323.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!