Skin biopsies were fixed in formalin and embedded in paraffin, sectioned at 4 µm and then stained using standard approaches with rabbit polyclonal anti-IL-22R1 antibody (1:100, Abcam, UK). All positive staining from the epidermis was counted using Image-Pro Plus, version 6.0.
Anti il 22r1 antibody
Manufactured by Abcam
Sourced in United Kingdom
Anti-IL-22R1 antibody is a laboratory reagent used for the detection and quantification of the Interleukin-22 Receptor Alpha 1 (IL-22R1) protein. It is a specific antibody that binds to IL-22R1, allowing researchers to identify and measure the expression of this receptor in various biological samples.
Automatically generated - may contain errors
3 protocols using anti il 22r1 antibody
1
Quantitative Analysis of IL-22R1 Expression
2
Immunohistochemical Analysis of Lung Tissue
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Lung was fixed with 10% neutral buffered formalin for 24 h before paraffin embedding. Sections were stained with anti-IL-22R1 antibody (Abcam), anti-phospho-STAT3 antibody (Cell Signaling Technology), or anti-Reg3γ antibody (Abcam) according to standard protocols.
3
Immunohistochemical Detection of Apoptosis Regulators
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Cells were incubated with anti-BAX antibody (Biolegend, Cat# 633602) or anti-BCL-2 antibody (Biolegend, Cat# 633502) or anti-phospho-NFAT5 (Ser145) antibody (Invitrogen, Cat# PA5-105436) or anti-IL-20 antibody (7E) or anti-IL-20R1 antibody (Abcam, Cat# ab90935) or anti-IL-20R2 antibody (Abcam, Cat# ab95824) or anti-IL-22R1 antibody (Abcam, Cat# ab5984) at 4 °C overnight. The next day, the sections were washed with PBS and incubated with the secondary antibody for 1 h. Reactions were detected using AEC chromogen stain (ScyTek Laboratories, Cat# ACG500) and nuclei were counterstained with hematoxylin (ScyTek Laboratories, Cat# HMM500).
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