After transfection, cells were plated in 96‐well plates with a density of 2×104 cells/well at 37℃, 5% CO2. After incubation for 48 h, cells were stained with Annexin V/FITC and PI reagent (BD, USA) at room temperature in the dark for 20 min. The apoptotic cells were measured by FACS Calibur flow cytometer (BD Biosciences, USA) as per the protocol.
Annexin 5 fitc and pi reagent
Manufactured by BD
Sourced in United States
Annexin V/FITC and PI reagent is a laboratory product used for the detection and analysis of apoptosis in cell samples. It utilizes the binding properties of Annexin V, a calcium-dependent phospholipid-binding protein, which has a high affinity for phosphatidylserine that is exposed on the surface of apoptotic cells. The reagent also contains propidium iodide (PI), a dye that intercalates with DNA and is used to identify late-stage apoptotic or necrotic cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using annexin 5 fitc and pi reagent
1
Apoptosis Assay in Cultured Cells
2
Evaluating miR-1305-induced Apoptosis
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Cells were transfected with miR-1305 mimics or control miRNA for 48 hrs. After that, cells were harvested by trypsinization and rinsed with pre-cold PBS. To evaluate the cell apoptosis, cells were resuspended in the binding buffer and stained with Annexin V-FITC and PI reagent (BD Biosciences, USA) in the darkness at room temperature (RT). The cell apoptosis was quantified with the flow cytometry (BD Biosciences, USA).
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