50:50 Poly(DL-lactide-co-glycolide) ester terminated (PLGA) was purchased from LACTEL (Birmingham, AL; Cat. No. B6010-1P, Lot# A17-142, weight average MW, 27.2 kDa, intrinsic viscosity (i.v.) 0.38, polydispersity index (PDI) 1.81). N-methyl-2-pyrrolidone (NMP, (USP)) was received from ASHLAND (Wilmington, DE, USA, Product Code 830697, 100%NMP). Dolutegravir base (DTG), rilpivirine base (RPV), darunavir base (DRV), ritonavir base (RTV), etravirine base (ETV), efavirenz base (EFV), raltegravir base (RAL) and idarubicin hydrochloride salt (IDA) were purchased from Selleckchem (Houston, TX, USA). Gemcitabine base (GEM) was purchased from LC Laboratories (Woburn, MA, USA). Lamivudine base (3TC) was purchased from Fisher Scientific (Pittsburgh, PA, USA). Zidovudine base (ZDV) was purchased from MedChemExpress (Monmouth Junction, NJ, USA). 5-fluorouracil base (5FU), phosphate-buffered saline (0.01 M PBS, pH 7.4), solutol-HS, HPLC grade acetonitrile (ACN), and water were purchased from Sigma Aldrich (St. Louis, MO, USA).
Hplc grade acetonitrile acn
Manufactured by Merck Group
Sourced in Germany, United States, India, Poland
HPLC-grade acetonitrile (ACN) is a high-purity organic solvent commonly used as a mobile phase component in high-performance liquid chromatography (HPLC) applications. It is a clear, colorless liquid with a characteristic odor. HPLC-grade ACN is highly purified and meets the stringent specifications required for HPLC analysis, ensuring consistent and reliable results.
Automatically generated - may contain errors
Lab products found in correlation
Formic acid, by Merck Group (19 mentions)
Methanol meoh, by Merck Group (18 mentions)
Methanol, by Merck Group (15 mentions)
Acetic acid, by Merck Group (10 mentions)
Milli q water, by Merck Group (7 mentions)
Ammonium formate, by Merck Group (5 mentions)
Milli q water purification system, by Merck Group (5 mentions)
Milli q system, by Merck Group (5 mentions)
Formic acid, by Thermo Fisher Scientific (4 mentions)
Cnw poly sery max spe cartridges, by ANPEL (3 mentions)
Milli q purification system, by Merck Group (3 mentions)
Methanol, by Thermo Fisher Scientific (3 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (2 mentions)
Milli q plus purification system, by Merck Group (2 mentions)
Tetrahydrofuran thf, by Merck Group (2 mentions)
Milli q, by Merck Group (2 mentions)
Trifluoroacetic acid, by Merck Group (2 mentions)
Dithiothreitol (dtt), by Merck Group (2 mentions)
Gallic acid, by Merck Group (2 mentions)
Folin ciocalteu reagent, by Merck Group (2 mentions)
75 protocols using hplc grade acetonitrile acn
1
Polymer-based Formulations for Drug Delivery
2
Somatostatin Digestion and Analysis
3
Synthesis and Characterization of Bile Acid Derivatives
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UniPR129 (N-(3α-hydroxy-5β-cholan-24-oyl)-L-β-homotryptophan), UniPR500 (N-(3-hydroxyimino-cholan-24-oyl)-L-β-homotryptophan), UniPR126, and UniPR141, employed as internal standards, were synthesized in our labs as previously described [8 (link),9 (link),11 (link)]. Mouse liver (MLM, from CD1 mice, pooled male) microsomes for phase I metabolism and human (pooled male and female) liver S9 fraction (HLS9) and mouse liver S9 fraction (MLS9 from CD1 mice, pooled male) for phase II metabolism were obtained from Xenotech, LLC (Cambridge, Kansas City, KS, USA). Glucose-6-phosphate (G6P), oxidized nicotinamide-adenine-dinucleotide phosphate (NADP+), Uridin-di-phosphoglucuronic acid (UDPGA), 3-phosphoadenosin-5-phosphate (PAPS), magnesium chloride (MgCl2), dithiothreitol (DTT), and glucose-6-phosphate-dehydrogenase (G6PDH) were supplied by Sigma Aldrich (Milan, Italy). 85% v/v formic acid was provided by ACEF Spa (Piacenza, Italy); HPLC-grade acetonitrile (ACN) and dimethyl sulfoxide (DMSO), >99.9% purity, were supplied by Sigma Aldrich (Milan, Italy) and VWR Chemicals (Radnor, PE, USA), respectively. Ultra-pure Millipore water (Darmstadt, Germany) was employed for HPLC mobile phase and sample preparations.
4
Purification and Analysis of Genetic Engineering Products
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Genetic engineering products: Wizard Plus SV Minipreps DNA Purification System was purchased from Promega Corp. (Madison, WI, USA); Not I restriction enzyme from Thermo Fisher Scientific (Waltham, MA, USA), mMESSAGE mMACHINE kit for high yield capped RNA transcription from Ambion Inc. (Austin, TX, USA); QIAquick PCR Purification and RNeasy Mini kits from Qiagen (Hilden, Germany). Gentamycin was obtained from Life Technologies Co. (Grand Island, NY, USA). High Performance Liquid Chromatography (HPLC) reagents: Trifluoroacetic acid (TFA) was acquired from Thermo Scientific (Rockford, IL, USA); HPLC-grade acetonitrile (ACN) from Sigma Chemical Co. (St. Louis, MO, USA). Bradford protein assay kit from Thermo Scientific (Rockford, IL, USA): oxidized insulin B chain, bovine serum albumin (BSA), type I collagenase, and acetylcholine (ACh) from Sigma Chemical Co. (St. Louis, MO, USA). All other reagents were analytical grade.
5
Improved Geraniol Solubility in Mice
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Geraniol (purity > 98%), was obtained from Sigma Chemical Co. (St. Louis, MO), and its solubility was improved by emulsifying in anhydrous glycerol according to the previously reported method (Pavan et al., 2018 (link)). Specific pathogen-free (SPF) Kunming (KM) mice (male, body weight, 20 ± 2 g) were provided by Sichuan Chengdu Institute of Biological Products Co., Ltd. (Chengdu, China). The Escherichia coli strain ATCC25922 was obtained from the American Type Culture Collection (ATCC, Rockville, MD, USA). The Geraniol standard (purity > 98%, No. SHBL2152), HPLC-grade acetonitrile (ACN), and methanol (MeOH) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Cephalexin (purity > 98%, No. 130408-201411) and kanamycin (purity > 98%, No. 130556-201502) were obtained from the National Institute for Food and Drug Control (Beijing, China).
6
Metabolite Analysis Using HPLC-Grade Solvents
7
Quantitative Proteomics using iTRAQ
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The 8-plex iTRAQ reagents were purchased from ABsciex (Framingham, MA, USA), and sequencing-grade trypsin was purchased from Promega (Madison, WI, USA). A TripleTOF 5600 mass spectrometer from ABsciex and an HPLC system from Waters (Milford, MA, USA) were used. For western blotting, the primary antibodies for RPL35 (ab190162) and RPS29 (ab56224) were purchased from Abcam (Cambridge, UK). ConA agarose, HPLC-grade acetonitrile (ACN), trifluoroacetic acid, formic acid, iodoacetamide, ammonium bicarbonate, and dithiothreitol were purchased from Sigma (St. Louis, MO, USA).
8
Breast Cancer Cell Cytotoxicity Assay
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TEOS, CTAB, dimethyl sulfoxide (DMSO), 3-(4,5-dimethylthoazol-2yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT), carbamazepine, and high-performance liquid chromatography (HPLC) grade acetonitrile (ACN) were purchased from Sigma Aldrich (St. Louis, MO, USA). Ethanol, toluene, NaOH, hydrochloric acid (HCl, 36–38 v/v%), and formic acid (>99.0%) were purchased from Samchun Pure Chemical (Pyungtaek, Korea). Human breast cancer cell (MCF-7) line was obtained from Korean cell line bank (Seoul, Korea). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin–streptomycin, and trypsin–EDTA were purchased from Gibco® BRL (Gaithersburg, MD, USA). DOX was provided as a gift from Korea United Pharm Inc. (Seoul, Korea).
9
Analytical Standards Preparation for Metabolomics
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Optima™ LC/MS grade formic acid and HPLC grade water were purchased from Fisher Scientific (Ottawa, ON, Canada). Optima™ LC/MS grade ammonium acetate, phenylisothiocyanate (PITC), 3-nitrophenylhydrazine (3-NPH), HPLC grade methanol and HPLC grade acetonitrile (ACN) were also purchased from Sigma-Aldrich (Oakville, ON, Canada). Furthermore, 2H-, 13C-, and 15N-labelled compounds were purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA, USA) and from Sigma-Aldrich (Oakville, ON, Canada).
A working internal standard (ISTD) solution mixture in water (for amino acids, biogenic amines, carbohydrates, carnitines and derivatives, phosphatidylcholines and their derivatives) was made by mixing all the prepared isotope-labeled stock solutions together. For organic acids, a working internal standard (ISTD) solution mixture in 75% aqueous methanol was made. All standard solutions were aliquoted and stored at −80 °C until further use.
A working internal standard (ISTD) solution mixture in water (for amino acids, biogenic amines, carbohydrates, carnitines and derivatives, phosphatidylcholines and their derivatives) was made by mixing all the prepared isotope-labeled stock solutions together. For organic acids, a working internal standard (ISTD) solution mixture in 75% aqueous methanol was made. All standard solutions were aliquoted and stored at −80 °C until further use.
10
Antimicrobial and Antioxidant Evaluation
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Gallic acid, vanillin, quercetin, ethanol, methanol, HPLC‐grade acetonitrile (ACN), acetic acid, Folin–Ciocalteu reagent, aluminum chloride, 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), and other chemicals were purchased from Sigma‐Aldrich (St. Louis, MO, USA). The purity of standard compounds was checked by HPLC–DAD analysis and was higher than 98%. All the other reagents were of analytical grade and purchased from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Bacillus subtilis (No. ATCC10160), Staphylococcus aureus (No. ATCC25923), Listeria monocytogenes (No. ATCC19120) were purchased from the Microbial Strain Preservation Center, China. Escherichia coli (DH5α) was prepared and stored at −20ºC.
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