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C57bl 6j male

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C57BL/6J males are a widely used inbred mouse strain. They are commonly used as control animals in research studies.

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Lab products found in correlation

B6 cg crhtm1 cre zjh j, by Jackson ImmunoResearch (3 mentions) C57bl 6j female, by Jackson ImmunoResearch (3 mentions) C57bl 6j, by Jackson ImmunoResearch (3 mentions) Haloperidol, by Merck Group (2 mentions) Cd1 females, by Charles River Laboratories (2 mentions) C57bl 6j female mice, by Jackson ImmunoResearch (1 mentions) Ain 93g, by Inotiv (1 mentions) Cx3cr1 gfp, by Jackson ImmunoResearch (1 mentions) Ntac sd females, by Taconic Biosciences (1 mentions) B6 cg foxp3sf j mice, by Jackson ImmunoResearch (1 mentions) Angiotensin 2, by Merck Group (1 mentions) Alzet model 1007d, by Durect (1 mentions) Cd1 retired male breeders, by Charles River Laboratories (1 mentions) D12450bi, by Research Diets (1 mentions) D12492, by Research Diets (1 mentions) Tamoxifen, by Research Diets (1 mentions) Tamoxifen, by Merck Group (1 mentions) Female balb cj, by Jackson ImmunoResearch (1 mentions) Sprague dawley rat, by Charles River Laboratories (1 mentions)

40 protocols using c57bl 6j male

1

Acclimating C57BL/6J and FVB/NJ Mice

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C57BL/6J males (Jackson Laboratory, 000664) and FVB/NJ males and females (Jackson Laboratory, 001800) were purchased at 5 weeks of age and used for experiments after one week of acclimation. All animals were maintained under pathogen-free conditions and cared for in accordance with the International Association for Assessment and Accreditation of Laboratory Animal Care policies and certification.
Rahmy S., Mishra S.J., Murphy S., Blagg B.S, & Lu X. (2022). Hsp90β inhibition upregulates interferon response and enhances immune checkpoint blockade therapy in murine tumors. Frontiers in Immunology, 13, 1005045.
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2

Crh-IRES-Cre Mouse Protocol

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C57BL/6J males were purchased from the Jackson Laboratories (stock #000664) or from Scripps Research rodent breeding colony. Crh-IRES-Cre breeders were obtained from The Jackson Laboratory (B6(Cg)Crhtm1(cre)Zjh/J, stock # 012704,34 (link)). All Crh-IRES-Cre mice used for experimentation were heterozygous males. Additional details are provided in the Supplement. All procedures adhered to the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Institutional Animal Care and Use Committee of Scripps Research.
Kreifeldt M., Herman M.A., Sidhu H., Okhuarobo A., Macedo G.C., Shahryari R., Gandhi P.J., Roberto M, & Contet C. (2022). Central amygdala corticotropin-releasing factor neurons promote hyponeophagia but do not control alcohol drinking in mice. Molecular psychiatry, 27(5), 2502-2513.
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3

Maternal Alcohol Exposure in Mice

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Five-week-old C57BL/6J female mice (Jackson Laboratories (Bar Harbor, ME, USA)), consumed the fixed-nutrient, purified diet AIN-93G (TD.94045, Envigo Teklad, Madison, WI, USA; [30 (link)]) throughout the study. At 8 weeks of age, n = 9 mice per treatment group were mated overnight with C57BL/6J males (Jackson Laboratories). The morning of vaginal plug detection was defined as embryonic day (E) 0.5 (E0.5). Starting at E8.5, pregnant females received 3 g/kg alcohol (ALC; 200 proof alcohol, USP grade; Decon Labs, King of Prussia, PA, USA) or isocaloric maltodextrin (CON; LoDex-10; #160175, Envigo Teklad, Madison, WI, USA), daily via oral gavage. On E17.5, two hours after the last gavage, mice were euthanized by isoflurane overdose and their tissues were flash-frozen for analysis. The maternal liver samples are 9 biological replicates per group, and fetal liver samples are the corresponding pool of four fetuses from these 9 dams. Samples were weighed at collection. All protocols were approved by the Institutional Animal Care and Use Committee of the David H. Murdoch Research Institute.
Saini N., Virdee M.S., Helfrich K.K., Kwan S.T., Mooney S.M, & Smith S.M. (2022). Untargeted Metabolome Analysis Reveals Reductions in Maternal Hepatic Glucose and Amino Acid Content That Correlate with Fetal Organ Weights in a Mouse Model of Fetal Alcohol Spectrum Disorders. Nutrients, 14(5), 1096.
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4

Catalepsy Induction in C57BL/6J Mice

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To test for catalepsy, C57BL/6J males (Jackson Laboratory) of approximately 9 weeks of age were injected IP with 10 ml/kg haloperidol (Sigma Aldrich) or finazine1 prepared in 10% DMSO in saline. As a control, 10% DMSO was used as a vehicle injection. Recording of time in a cataleptic position was carried out at 30, 60, 90, 120, and 180 min after injection. Catalepsy was defined as the amount of time spent on a horizontal steel rod (~0.5 cm in diameter, positioned ~4.5 cm above the surface of the testing surface). The time during which each mouse maintained this position was recorded up to a maximum of 2 min. Following each cataleptic measurement, mice were observed for clinical signs. The following signs were recorded as present or absent for each mouse at each time point: Lethality, Convulsions, Tremor, Straub Tail, Excitation, Abnormal Gait, Jumps, Writhes, Stereotypy, Head Twitches. Data were analyzed by ANOVA followed by Fisher’s LSD post hoc test.
Bruni G., Rennekamp A.J., Velenich A., McCarroll M., Gendelev L., Fertsch E., Taylor J., Lakhani P., Lensen D., Evron T., Lorello P.J., Huang X.P., Kolczewski S., Carey G., Caldarone B.J., Prinssen E., Roth B.L., Keiser M.J., Peterson R.T, & Kokel D. (2016). Zebrafish behavioral profiling identifies multi-target antipsychotic-like compounds. Nature chemical biology, 12(7), 559-566.
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5

Notch Signaling Analysis in Mice

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Studies were approved by the Columbia University Institutional Animal Care and Use Committee (IACUC #AC-AAAE265). For assessment of Notch signaling activity, we bred CBF:H2B-Venus transgenic virgin females (Jackson Laboratories) [26 (link)] with C57BL/6J males (Jackson Laboratories). For assessment of wild type expression patterns, we bred C57BL/6J virgin female mice and C57BL/6J males. Noon on the day a mating plug was observed was designated as E0.5. Uterine implantation sites from pregnant females at E8.5 and placentas from pregnant females at E10.5, E12.5 and E18.5 were analyzed.
Levin H.I., Sullivan-Pyke C.S., Papaioannou V.E., Wapner R.J., Kitajewski J.K., Shawber C.J, & Douglas N.C. (2017). Dynamic maternal and fetal Notch activity and expression in placentation. Placenta, 55, 5-12.
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6

Diet-Induced Obesity in C57BL/6J Mice

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Four-week-old C57BL/6J males were purchased from The Jackson Laboratory and acclimated for 2 weeks. Mice were isocalorically fed a control mouse or American diet for 90 days at TAMU. Ten males were used per group, as males had a more pronounced difference in adiposity between the control and American diets. Mice were housed five per cage and fed 11.5 kcal/mouse daily. Prior experiments optimized food quantity to ensure all food was eaten. Mice were weighed and body composition analyzed (EchoMRI-130 Body Composition Analyzer).
Barrington W.T., Wulfridge P., Wells A.E., Rojas C.M., Howe S.Y., Perry A., Hua K., Pellizzon M.A., Hansen K.D., Voy B.H., Bennett B.J., Pomp D., Feinberg A.P, & Threadgill D.W. (2017). Improving Metabolic Health Through Precision Dietetics in Mice. Genetics, 208(1), 399-417.
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7

Sperm and Oocyte Collection from Mice

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Mouse sperm samples were collected from 12–14-week-old C57BL/6J males (Jackson Laboratory, Farmington, CT, USA). Mouse cumulus–oocyte complexes (COCs) were collected from 8–10-week old super ovulated CD1 females (Charles River Laboratories, Wilmington, MA, USA). For superovulation, females were first injected with 7.5–10 IU of pregnant mare serum gonadotropin [PMSG] (Lee BioSolutions, cat # 493-10, Maryland Heights, MO, USA) followed by 7.5–10 IU of human chorionic gonadotrophin [hCG] (Sigma, cat #CG5, St. Louis, MO, USA) 48 h later. COCs were collected 13 h post-hCG injections. Animal care and use of experimental animals were conducted in accordance with specific guidelines and standards dictated by the Office of Laboratory Animal Welfare (OLAW) and approved by the Institutional Animal Use and Care Committee (IACUC), University of Massachusetts Amherst (Protocol #2019-0008).
Tourzani D.A., Yin Q., Jackson E.A., Rando O.J., Visconti P.E, & Gervasi M.G. (2022). Sperm Energy Restriction and Recovery (SER) Alters Epigenetic Marks during the First Cell Cycle of Development in Mice. International Journal of Molecular Sciences, 24(1), 640.
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8

Thymidine Analog Labeling in Mice

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CldU (Sigma) and IdU (MP Biomedicals) were dissolved at 1mg/ml [3 (link)] and stored at 4°C for no longer than two weeks. Thymidine analog solutions were administrated via drinking water and were protected from light. For labeling periods longer than 1 day, thymidine analog solutions were renewed every other day. Experimental mice were C57BL6/J males (Jackson Laboratories), 7-week-old at the beginning of administration of thymidine analogs.
Pignon J.C., Grisanzio C., Carvo I., Werner L., Regan M., Wilson E.L, & Signoretti S. (2015). Cell Kinetic Studies Fail to Identify Sequentially Proliferating Progenitors as the Major Source of Epithelial Renewal in the Adult Murine Prostate. PLoS ONE, 10(5), e0128489.
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9

Catalepsy Induction in C57BL/6J Mice

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To test for catalepsy, C57BL/6J males (Jackson Laboratory) of approximately 9 weeks of age were injected IP with 10 ml/kg haloperidol (Sigma Aldrich) or finazine1 prepared in 10% DMSO in saline. As a control, 10% DMSO was used as a vehicle injection. Recording of time in a cataleptic position was carried out at 30, 60, 90, 120, and 180 min after injection. Catalepsy was defined as the amount of time spent on a horizontal steel rod (~0.5 cm in diameter, positioned ~4.5 cm above the surface of the testing surface). The time during which each mouse maintained this position was recorded up to a maximum of 2 min. Following each cataleptic measurement, mice were observed for clinical signs. The following signs were recorded as present or absent for each mouse at each time point: Lethality, Convulsions, Tremor, Straub Tail, Excitation, Abnormal Gait, Jumps, Writhes, Stereotypy, Head Twitches. Data were analyzed by ANOVA followed by Fisher’s LSD post hoc test.
Bruni G., Rennekamp A.J., Velenich A., McCarroll M., Gendelev L., Fertsch E., Taylor J., Lakhani P., Lensen D., Evron T., Lorello P.J., Huang X.P., Kolczewski S., Carey G., Caldarone B.J., Prinssen E., Roth B.L., Keiser M.J., Peterson R.T, & Kokel D. (2016). Zebrafish behavioral profiling identifies multi-target antipsychotic-like compounds. Nature chemical biology, 12(7), 559-566.
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10

Chronic Cranial Window Mice Study

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Mice were C57BL/6J males (Jackson Laboratories) or CX3CR1-GFP heterozygotes (Jackson Laboratories stock #008451) (Jung et al., 2000 (link)) that were 8 to 12 weeks-old at the beginning of the experiment. Mice were singly housed after chronic cranial window implantation or housed with littermates for experiments that did not require an implant. In both cases, they were housed on a 12 hr reversed light/dark cycle after window implantation or viral injection. Littermates were randomly assigned to experimental groups (control vs. PLX). All procedures were performed using approved protocols in accordance with institutional (Harvard University Institutional Animal Care and Use Committee) and national guidelines.
Wallace J., Lord J., Dissing-Olesen L., Stevens B, & Murthy V.N. (2020). Microglial depletion disrupts normal functional development of adult-born neurons in the olfactory bulb. eLife, 9, e50531.
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