Zorbax sb c8 column

Manufactured by Agilent Technologies

Sourced in United States

The Zorbax SB-C8 column is a reversed-phase high-performance liquid chromatography (HPLC) column designed for the separation and analysis of a wide range of compounds. The column features a silica-based stationary phase with n-octyl (C8) functional groups, providing a moderate hydrophobic interaction with analytes. The Zorbax SB-C8 column is suitable for a variety of applications, including the separation of small molecules, peptides, and proteins.

Automatically generated - may contain errors

Lab products found in correlation

22 protocols using zorbax sb c8 column

Quantitative LC-MS/MS for Anti-TB Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols

A region-specific
drug quantitation
was performed using LCM liquid chromatography with tandem mass spectrometry
quantitation (LC-MS/MS) as previously described.^{41 (link)} Briefly, 3 × 10⁶ μm² regions
of mimetic, caseum, and normal lung were dissected and collected in
polymerase chain reaction (PCR) tubes for a liquid–liquid extraction
and LC-MS/MS quantitation. LC-MS/MS analysis was performed on a Sciex
QTRAP 6500+ triple-quadrupole mass spectrometer coupled to a Shimadzu
Nexera X2 UHPLC system. Chromatographic separation was performed on
an Agilent Zorbax SB-C8 column (2.1 × 30 mm; particle size, 3.5
μm) using a reverse-phase gradient elution of 0.1% formic acid
(FA) in Milli-Q water (mobile phase A) and 0.1% FA in acetonitrile
(mobile phase B) for Rifampicin, rifapentine, rifabutin and pretomanid.
For Doxycycline and Kanamycin, 0.1% heptafluorobutyric acid (HFBA)
was added to each mobile phase. Multiple-reaction monitoring (MRM)
of precursor/fragment transitions in electrospray positive-ionization
mode was used to quantify the analytes. Data processing was performed
using Analyst software (ver. 1.7.1; Applied Biosystems Sciex). Technical
triplicates (n = 3) were quantified for each drug.

Wang N., Sarathy J.P., Zimmerman M., Kaya F., Wang H., Dartois V, & Carter C.L. (2021). On-Slide Heat Sterilization Enables Mass Spectrometry Imaging of Tissue Infected with High-Threat Pathogens Outside of Biocontainment: A Study Directed at Mycobacterium tuberculosis. Journal of the American Society for Mass Spectrometry, 32(11), 2664-2674.

+ Open protocol

+ Expand

Quantification of S1P in PRP and PPP

Check if the same lab product or an alternative is used in the 5 most similar protocols

After thawing of frozen PRP and PPP samples, the level of S1P has been measured by liquid chromatography-tandem mass spectrometry as previously described [87 (link)]. In brief, 20 μL of PRP and PPP respectively were incubated with 20 μL of the internal standard (1 μM [16,17,18-²H₇]-S1P (S1P-d₇, Avanti Polar Lipids, Alabaster, AL, USA). Subsequently, proteins were precipitated with 350 μL of acetonitrile/water, 80/20, vol/vol. After centrifugation at 10,000 g for 15 min the extracts were subjected to reverse-phase chromatography on a Zorbax SB-C8 column (2.1 × 50 mm; Agilent Technologies, Santa Clara, CA, USA) at a flow rate of 0.35 mL/min. S1P was eluted with a binary gradient for 6 min (methanol/acetonitrile/0.1% formic acid, 2.5/2.5/95, vol/vol/vol to methanol/acetonitrile/0.1% formic acid, 30/30/40, vol/vol/vol) and measured by tandem mass spectrometry (Varian L1200 MS/MS, Agilent Technologies, Waldbronn, Germany) in the multiple reaction mode, monitoring the [M+H]⁺ S1P parent ion (m/z = 380) fragmentation to the daughter ion m/z = 264. The internal standard S1P-d₇ with the m/z 387 to 271 transition was used to correct for variations in sample preparation and instrument response. Calibration curves (four levels of S1P: 0; 0.1; 0.3; 1; 3 μmol/L) were generated to calculate absolute S1P concentrations in PRP and PPP samples.

Marx S., Splittstöhser M., Kinnen F., Moritz E., Joseph C., Paul S., Paland H., Seifert C., Marx M., Böhm A., Schwedhelm E., Holzer K., Singer S., Ritter C.A., Bien-Möller S., Schroeder H.W, & Rauch B.H. (2018). Platelet activation parameters and platelet-leucocyte-conjugate formation in glioblastoma multiforme patients. Oncotarget, 9(40), 25860-25876.

+ Open protocol

+ Expand

Optimized HPLC Separation of 5 Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The aim of the optimization was to develop a single method able to separate the 5 drugs with appropriate resolution factor and without interferences with potential degradation products. In order to achieve this goal, several chromatographic parameters such as mobile phase composition, column, pH, temperature or detection wavelength were appropriately chosen and optimized. Separation was considered as appropriate if resolutions between two peaks were all superior to 1.5. A desirable separation and resolution was obtained using a mobile phase composed of methanol (solvent A) and 0.1% formic acid in water (solvent B) set in a gradient mode (0–10 min: 80% A; 10–17 min: 80% → 30% A; 17–22 min: 30% → 80% A); the flow rate was set at 0.8 mL/min and the injection volume at 40 μL. The selected column was an Agilent Zorbax SB-C8 column, 5µm × 4.6 × 250 mm, maintained at 25 °C ± 1 °C. The vials were thermostated at 8 °C during the LC process. Due to the shortening of width of Sufentanil absorption in the UV region, the detection wavelength was set at 205 nm. The other 4 molecules were detected and quantified at 205 and 225 nm.

Tarantini M.G., Ramos S., Secrétan P.H., Guichard L., Hassani L., Bellanger A., Mayaux J., Tilleul P., El Kouari F, & Sadou Yayé H. (2022). Therapeutic Treatment Plan Optimization during the COVID-19 Pandemic: A Comprehensive Physicochemical Compatibility Study of Intensive Care Units Selected Drugs. Pharmaceutics, 14(3), 550.

+ Open protocol

+ Expand

Quantification of Noradrenaline by HPLC-MS/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

A method for the determination of noradrenaline was established by high-performance liquid chromatography-tandem mass spectrometry. The noradrenaline standard was detected and analyzed by gradient. After blood samples were collected, the serum was separated and frozen at -80 °C. After thawing, methanol was added, and then acetonitrile was added after the internal standard solution was vortex centrifuged again. After stratification, the upper organic phase was placed in another tube, and 10 μL of the organic phase was taken for determination. Chromatographic conditions are as follows: Agilent Zorbax sb-c8 column (2.1 mm × 100 mm, 1.8 μm); mobile phase, water (containing 0.1% formic acid, 2 mmol/L ammonium acetate): Acetonitrile (containing 0.1% formic acid) = 55:45; flow rate, 0.4 mL/min; column temperature, 30 °C; injection volume, 2 μL; autosampler temperature, 10 °C. For mass spectrometry, the ionization mode was electrospray ionization, multireaction ion detection MRM was used in the positive ion mode, the capillary voltage was 3.5 kV, the temperature of the dryer was 350 °C, the flow rate of the dryer was 5 L/min, the atomization gas was 60 psi, the sheath gas temperature was 350 °C, and the sheath gas flow rate was 11 L/min.

Hong Y., Zhao J., Chen Y.R., Huang Z.H., Hou L.D., Shen B, & Xin Y. (2022). Spinal anesthesia alleviates dextran sodium sulfate-induced colitis by modulating the gut microbiota. World Journal of Gastroenterology, 28(12), 1239-1256.

+ Open protocol

+ Expand

Quantitative Analysis of BCP via LC-MS/MS

Check if the same lab product or an alternative is used in the 5 most similar protocols

To analyze BCP from the in vivo and in vitro studies, the LC–MS/MS manufactured by Shimadzu (LCMS-8050 set, Kyoto, Japan) was used. The LC–MS/MS system consisted of an auto-sampler (SIL-30AC), LC pump (LC-30AD-1 and -2), column oven (CTO-20AC), and a coupled electrospray ionization (ESI) detector (LCMS-8050). A Zorbax SB-C8 column (150 × 2.1 mm, i.d. 3.5 µm, Agilent, Santa Clara, CA, USA) with Security Guard Cartridges RP-1 (4 × 3.0 mm; Phenomenex, CA, USA) was used to separate each compound. The mobile phase was adjusted using 95% ACN in 0.1% formic acid. Flow rate, column oven temperature, and injection volume were 0.5 mL/min, 40 °C, and 5 L, respectively. The electrospray ionization (ESI) source was operated in the negative mode for BCP and in the positive mode for IS. All samples were observed in multiple reaction monitoring (MRM) modes. In the MRM mode, the dwell time was 100 ms per MRM channel. Gas flow, source temperature, and nebulizing gas flow were set at 10 L/min, 300 °C, and 3 L/min, respectively. The collision energy was 26 V for BCP and −14 V for IS. The analytical conditions are summarized in Table S3.

Lee Y.J., Kim H.Y., Pham Q.L., Lee J.D, & Kim K.B. (2022). Pharmacokinetics and the Dermal Absorption of Bromochlorophene, a Cosmetic Preservative Ingredient, in Rats. Toxics, 10(6), 329.

+ Open protocol

+ Expand

Quantification of Serum Sphingosine-1-Phosphate

Check if the same lab product or an alternative is used in the 5 most similar protocols

Blood samples from controls and patients were processed the same way. After coagulation at 4 °C, samples were cleared by centrifugation and serum immediately frozen and stored at −80 °C until S1P was measured. Serum-S1P measurements were performed by using a previously described protocol with minor modifications [17 (link)]. After the addition of internal standard (1 nmol/ml C17-S1P; Avanti Polar Lipids, Alabaster, AL, USA), serum was de-proteinated by the addition of acetonitrile (final concentration of 70 %). Extracts were cleared by centrifugation and subjected to reverse-phase chromatography on a Zorbax SB-C8 column (2.1 × 50 mm; Agilent Technologies, Santa Clara, CA, USA) at a flow rate of 0.35 ml/min. S1P was eluted by a binary gradient (2.5 % methanol, 2.5 % acetonitrile, 0.1 % formic acid to 30 % methanol, 30 % acetonitrile, 0.1 % formic acid; % = volume %) and measured by a Varian MS 1200 mass spectrometer by using multiple reaction mode in which the M + H S1P parent ion (m/z = 380) is fragmented to form a daughter ion at m/z = 264, which is then used for quantitation. The internal standard (C17-S1P) with the m/z 366-to-250 transition was used to correct for variations in sample preparation and instrument response. A calibration curve (0.1-3 nmol/ml S1P) was generated to calculate absolute S1P concentrations in samples.

Winkler M.S., Nierhaus A., Holzmann M., Mudersbach E., Bauer A., Robbe L., Zahrte C., Geffken M., Peine S., Schwedhelm E., Daum G., Kluge S, & Zoellner C. (2015). Decreased serum concentrations of sphingosine-1-phosphate in sepsis. Critical Care, 19, 372.

+ Open protocol

+ Expand

Sphingolipid Quantification via UHPLC/MS/MS

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

The sphingolipids content were measured with the use of UHPLC/MS/MS according to Blachnio-Zabielska et al. [23 (link)] with minor modifications. Briefly, muscle samples (~20 mg) were pulverized and then homogenized in a buffer consisting of 0.25 M sucrose, 25 mM KCl, 50 mM Tris, and 0.5 mM EDTA, pH 7.4. Immediately afterwards, a mixture of internal standards (Sph-d7, SPA-d7, S1P-d7, C15:0-d7-Cer, C16:0-d7-Cer, C18:1-d7-Cer, C18:0-d7-Cer, 17C/20:0-Cer, C24:1-d7-Cer, C24-d7-Cer Avanti Polar Lipids, Alabaster, Al) and extraction mixture (isopropanol:water:ethyl acetate, 30:10:60); v:v:v) have been added to each sample. After extraction, the samples were evaporated under a stream of nitrogen and suspended in LC Solvent B (2 mM ammonium formate, 0.1% formic acid in methanol) for UHPLC/MS/MS analysis. The chromatographic separation was performed using a reverse-phase Zorbax SB-C8 column 2.1 × 150 mm, 1.8 μm (Agilent Technologies, Santa Clara, CA, USA) in binary gradient using 1 mM ammonium formate, 0.1% formic acid in water as solvent A, and 2 mM ammonium formate, 0.1% formic acid in methanol as solvent B at the flow rate of 0.4 mL/min. Sphingolipids were analyzed using Sciex QTRAP 6500 + triple quadrupole mass spectrometer (AB Sciex Germany GmbH, Darmstadt, Germany) with multiple reaction monitoring (MRM) against standard curves constructed for each compound.

Błachnio-Zabielska A.U., Roszczyc-Owsiejczuk K., Imierska M., Pogodzińska K., Rogalski P., Daniluk J, & Zabielski P. (2022). CerS1 but Not CerS5 Gene Silencing, Improves Insulin Sensitivity and Glucose Uptake in Skeletal Muscle. Cells, 11(2), 206.

+ Open protocol

+ Expand

Region-Specific Drug Quantification in Lung Samples

Check if the same lab product or an alternative is used in the 5 most similar protocols

A region-specific drug quantitation was performed using LCM liquid chromatography with tandem mass spectrometry quantitation (LC-MS/MS) as previously described.⁴¹ Briefly, 3 × 10⁶μm² regions of mimetic, caseum, and normal lung were dissected and collected in polymerase chain reaction (PCR) tubes for a liquid–liquid extraction and LC-MS/MS quantitation. LC-MS/MS analysis was performed on a Sciex QTRAP 6500+ triple-quadrupole mass spectrometer coupled to a Shimadzu Nexera X2 UHPLC system. Chromatographic separation was performed on an Agilent Zorbax SB-C8 column (2.1 × 30 mm; particle size, 3.5 μm) using a reverse-phase gradient elution of 0.1% formic acid (FA) in Milli-Q water (mobile phase A) and 0.1% FA in acetonitrile (mobile phase B) for Rifampicin, rifapentine, rifabutin and pretomanid. For Doxycycline and Kanamycin, 0.1% heptafluorobutyric acid (HFBA) was added to each mobile phase. Multiple-reaction monitoring (MRM) of precursor/fragment transitions in electrospray positive-ionization mode was used to quantify the analytes. Data processing was performed using Analyst software (ver. 1.7.1; Applied Biosystems Sciex). Technical triplicates (n = 3) were quantified for each drug.

+ Open protocol

+ Expand

Partial Photolytic Cleavage of Zapalog

Check if the same lab product or an alternative is used in the 5 most similar protocols

To achieve partial photolysis, a 40µl sample of 1mM Zapalog in phosphate-buffered saline, pH 7.2, contained in a quartz cuvette, were exposed for 5s to ~15 mW of light from a 0.22 NA fiber optic cable (ThorLabs) coupled to a 405nm laser (Vortran Stradus 405–250; Vortran Laser Technology). Both purity and photolytic cleavage of Zapalog were confirmed by reverse-phase high-performance liquid chromatography (Agilent Technologies) on a 4.6 mm 150 mm Zorbax SB-C8 column (5 mm particle size) in H2O, CH3CN, 0.1% TFA at 1 ml/ min. Samples were monitored at 220 nm and 350 nm. A non-photolyzed zapalog sample was prepared and run under the same conditions. (Supplementary Figure S1a)

Gutnick A., Banghart M.R., West E.R, & Schwarz T.L. (2019). The light-sensitive dimerizer zapalog reveals distinct modes of immobilization for axonal mitochondria.. Nature cell biology, 21(6), 768-777.

+ Open protocol

+ Expand

Partial Photolytic Cleavage of Zapalog

Check if the same lab product or an alternative is used in the 5 most similar protocols

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!