Log In Sign up
The largest database of trusted experimental protocols

Smgm 2 smooth muscle growth medium 2

Manufactured by Lonza
Visit Supplier
Sourced in Switzerland

The SmGM-2 Smooth Muscle Growth Medium-2 is a cell culture medium designed to support the growth and maintenance of smooth muscle cells in vitro. It contains the necessary nutrients, growth factors, and other components to facilitate the optimal growth and proliferation of smooth muscle cells.

Automatically generated - may contain errors

Lab products found in correlation

Human epidermal growth factor (hegf), by Lonza (2 mentions) Hfgf b, by Lonza (2 mentions) Mirvana mirna isolation kit, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

SmGM-2 (60 citations) SmGM-2 medium (27 citations) Smooth muscle growth medium (5 citations) Smooth muscle growth medium (SMGM; (2 citations) SmGM-2TM smooth muscle growth medium-2 (2 citations)

4 protocols using smgm 2 smooth muscle growth medium 2

1

Human Vascular Smooth Muscle Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Human VSMCs were purchased from the American Type Culture Collection (ATCC® PCS-100-012™, Manassas, VA, USA). For the VSMC culture, SmGM-2 Smooth Muscle Growth Medium-2 (Lonza Group, Basel, Switzerland) was the required medium for propagating VSMCs along with 10% fetal bovine serum, as per the manufacturer's instructions. VSMCs were maintained in a 5% CO2 incubator at 37°C.
Yang B., Wang X., Ying C., Peng F., Xu M., Chen F, & Cai B. (2021). Long Noncoding RNA SNHG16 Facilitates Abdominal Aortic Aneurysm Progression through the miR-106b-5p/STAT3 Feedback Loop. Journal of Atherosclerosis and Thrombosis, 28(1), 66-78.
+ Open protocol
+ Expand
2

Characterization of UT-TERT and GM-TERT Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human myometrial (UT-TERT) and fibroid (GM-TERT) cell lines were a kind gift from Dr. John Risinger and have been characterized previously (Carney et al., 2002 (link)). UT-TERT cells were cultured and maintained in SmGM™-2 Smooth Muscle Growth Medium-2 containing 5% FBS, 0.1% insulin, 0.2% basic human fibroblast growth factor (hFGF-b), 0.1% GA-100, and 0.1% human epidermal growth factor (hEGF) (Lonza, Walkersville, MD).
George J.W., Fan H., Johnson B., Carpenter T.J., Foy K.K., Chatterjee A., Patterson A.L., Koeman J., Adams M., Madaj Z.B., Chesla D., Marsh E.E., Triche T.J., Shen H, & Teixeira J.M. (2019). Integrated Epigenome, Exome, and Transcriptome Analyses Reveal Molecular Subtypes and Homeotic Transformation in Uterine Fibroids. Cell reports, 29(12), 4069-4085.e6.
+ Open protocol
+ Expand
3

Characterization of UT-TERT and GM-TERT Cell Lines

Check if the same lab product or an alternative is used in the 5 most similar protocols
The human myometrial (UT-TERT) and fibroid (GM-TERT) cell lines were a kind gift from Dr. John Risinger and have been characterized previously (Carney et al., 2002 (link)). UT-TERT cells were cultured and maintained in SmGM™-2 Smooth Muscle Growth Medium-2 containing 5% FBS, 0.1% insulin, 0.2% basic human fibroblast growth factor (hFGF-b), 0.1% GA-100, and 0.1% human epidermal growth factor (hEGF) (Lonza, Walkersville, MD).
George J.W., Fan H., Johnson B., Carpenter T.J., Foy K.K., Chatterjee A., Patterson A.L., Koeman J., Adams M., Madaj Z.B., Chesla D., Marsh E.E., Triche T.J., Shen H, & Teixeira J.M. (2019). Integrated Epigenome, Exome, and Transcriptome Analyses Reveal Molecular Subtypes and Homeotic Transformation in Uterine Fibroids. Cell reports, 29(12), 4069-4085.e6.
+ Open protocol
+ Expand
4

Isolating Total RNA from Smooth Muscle Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
SMCs were propagated in SmGM-2 Smooth Muscle Growth Medium-2 (Lonza Group, Basel, Switzerland) containing 10% fetal bovine serum (FBS) according to the manufacturer's instructions. Cells were maintained in a humidified atmosphere at 37°C and 5% CO2. After 48 h, cells were harvested and total RNA isolated using the mirVana™ miRNA Isolation kit (Ambion; Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer's instructions.
Cao X., Cai Z., Liu J., Zhao Y., Wang X., Li X, & Xia H. (2017). miRNA-504 inhibits p53-dependent vascular smooth muscle cell apoptosis and may prevent aneurysm formation. Molecular Medicine Reports, 16(3), 2570-2578.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!