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4 well cell culture chamber slides

Manufactured by Corning
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The 4-well cell culture chamber slides are a laboratory equipment product designed for growing and maintaining cells in a controlled environment. The product provides a multi-well format for culturing cells, allowing for parallel experimentation and comparison of different conditions. The slides are made of high-quality materials suitable for cell culture applications.

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Lab products found in correlation

Lsm 880 confocal microscope, by Zeiss (4 mentions) Advanced dmem f12, by US Biological (2 mentions) Click it edu alexa fluor 488 imaging kit, by Thermo Fisher Scientific (2 mentions) Dmem f12, by US Biological (2 mentions)

2 protocols using 4 well cell culture chamber slides

1

Quantifying PDO Proliferation in Serine-Glycine Depletion

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PDOs were seeded on 4-well cell culture chamber slides (Corning, 354114) at equal numbers. PDO media was replaced with vehicle or serine and glycine-free media the next day and incubated for 10 days. Serine and glycine-free Advanced DMEM/F12 was generated using amino acid, glucose, and pyruvate free DMEM/F12 (US Biological, D9807-11) supplemented with ethanolamine, glutathione, ascorbic acid, transferrin, and AlbuMAX II to the levels found in commercial Advanced DMEM/F12. PDO cultures were treated with 10 μM 5-ethynyl-2′-deoxyuridine (EdU) for 4h and fixed for 40 minutes with 4% PFA. The fixed PDOs were permeabilized with 0.5% Tx-100 for 20 minutes and labeled using the Click-iT EdU Alexa Fluor 488 imaging kit (ThermoFisher, C10337) according to manufacturer’s instructions. Labeled PDOs were imaged with a Zeiss LSM880 confocal microscope. The ratio of EdU+ cells was quantified in 16 to 25 organoids/group by counting the number of EdU+ cells per total number of cells. Statistical analysis was performed with Prism GraphPad. Student’s t-test was used to calculate p values.
Choi B.H., Rawat V., Högström J., Burns P.A., Conger K.O., Ozgurses M.E., Patel J.M., Mehta T.S., Warren A., Selfors L.M., Muranen T, & Coloff J.L. (2022). Lineage-specific silencing of PSAT1 induces serine auxotrophy and sensitivity to dietary serine starvation in luminal breast tumors. Cell reports, 38(3), 110278.
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2

Quantifying PDO Proliferation in Serine-Glycine Depletion

Check if the same lab product or an alternative is used in the 5 most similar protocols
PDOs were seeded on 4-well cell culture chamber slides (Corning, 354114) at equal numbers. PDO media was replaced with vehicle or serine and glycine-free media the next day and incubated for 10 days. Serine and glycine-free Advanced DMEM/F12 was generated using amino acid, glucose, and pyruvate free DMEM/F12 (US Biological, D9807-11) supplemented with ethanolamine, glutathione, ascorbic acid, transferrin, and AlbuMAX II to the levels found in commercial Advanced DMEM/F12. PDO cultures were treated with 10 μM 5-ethynyl-2′-deoxyuridine (EdU) for 4h and fixed for 40 minutes with 4% PFA. The fixed PDOs were permeabilized with 0.5% Tx-100 for 20 minutes and labeled using the Click-iT EdU Alexa Fluor 488 imaging kit (ThermoFisher, C10337) according to manufacturer’s instructions. Labeled PDOs were imaged with a Zeiss LSM880 confocal microscope. The ratio of EdU+ cells was quantified in 16 to 25 organoids/group by counting the number of EdU+ cells per total number of cells. Statistical analysis was performed with Prism GraphPad. Student’s t-test was used to calculate p values.
Choi B.H., Rawat V., Högström J., Burns P.A., Conger K.O., Ozgurses M.E., Patel J.M., Mehta T.S., Warren A., Selfors L.M., Muranen T, & Coloff J.L. (2022). Lineage-specific silencing of PSAT1 induces serine auxotrophy and sensitivity to dietary serine starvation in luminal breast tumors. Cell reports, 38(3), 110278.
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