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40 protocols using na2so4

1

Synthesis of Substituted Benzene Derivatives

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1,4-Diethoxybenzene, paraformaldehyde,
1,2-dichloroethane, boron trifluoride etherate, chloroform, boron
tribromide, ethanol, potassium carbonate, anhydrous acetonitrile,
methyl chloroacetate, dichloromethane, anhydrous sodium sulfate, methanol,
ethylenediamine, n-hexane, and hydrochloric acid
were obtained from Aladdin Reagent Co. Ltd. Ag+, Al3+, Ba2+, Bi+, Ca2+, Cd2+, Co3+, Cs+, Cu2+, Fe2+, Fe3+, Hg2+, K+, Li+, Mg2+, Na+, Pb2+, Sb2+, Sn2+, Sr2+, Ni2+, Au3+, Br, I, PO43–, HPO42–, Cl, HSO3, CO32–, HCO3, SO42–, NO3, NO2, F (AgNO3, Al(NO3)3·9H2O, BaCl2·2HO2, Bi(NO3)3·5H2O, CaCl2, CdCl2, CoCl2·6H2O, Cs2CO3, CuSO4·5H2O, FeSO4·7HO2, FeCl3·6H2O, HgCl2, KCl, LiF, MgSO4·7H2O, Na2SO4, Pb(NO3)2, SbCl3, SnCl2·2H2O, SrCl2·6H2O, NiCl2·6H2O, HAuCl4, NaBr, NaI, Na3PO4, Na2HPO4, NaCl, NaHSO3, Na2CO3, NaHCO3, Na2SO4, NaNO3, NaNO2, and NaF) metal ions were purchased
from Sinopharm Chemical Reagent Co. Ltd. Deionized water was used
throughout all experiments.
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2

Synthesis of Ternary Molten Salt Eutectic Mixtures

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KNO3, NaNO3 and Na2SO4 used in this experiment were analytical grade. They were purchased from Sinopharm Chemical Reagent Beijing Co., Ltd. (Beijing, China).
NaNO3, KNO3 and Na2SO4 were dried in a constant-temperature air-drying oven at 80 °C for 24 h to remove moisture from raw materials. In this study, molten salts were synthesized by the static melting method. Briefly, 20 g binary salt of 60wt% NaNO3-40wt% KNO3 and ternary eutectic salt of TMS with different proportions, calculated according to the phase diagram in the literature [37 (link)], as shown in Table 3, were mixed into a 50 mL crucible. Then, the mixture salts were heated up to 300 °C with a heating rate of 5 °C/min in a muffle furnace and were kept at 300 °C for 4 h. Finally, the synthesized binary and ternary molten salts were poured out at 300 °C and cooled to room temperature naturally, and then crushed into powder, sealed and kept in desiccators. The preparation process is shown in Figure 19.
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3

Standardized Radioactivity Measurement Protocol

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The pure 241Am and 90Sr/90Y standard solutions were used for LSC calibration. 241Am in 0.5 mol L−1 HNO3 (radio-purity > 99.9%) was provided by China Institute of Atomic Energy. 90Sr/90Y in 3 g L−1 HNO3 was purchased from Czech Metrology Institute. Both of them were diluted with 3 g L−1 HNO3 carrier solution. Then their activities were certified by the national first-class ionizing radiation metrology station with the values of 20.5 ± 0.2 Bq g−1 and 31.1 ± 0.3 Bq g−1 (with coverage factor k = 2 for 95% confidence), respectively. 40K standard solution was prepared using KCl (guaranteed reagent, purity > 99.8%) supplied by Macklin Biochemical Co. Ltd. (Shanghai, China). And the activity concentration of 40K was determined by using the ratio between natural and radioactive potassium. Cocktail Ultima Gold AB (PerkinElmer) and 20 mL polyethylene vials (PerkinElmer) were used for the LSC measurement.
Five solid salts of NaCl, MgCl2, CaCl2, Na2SO4, and NaHCO3 (analytical reagent) were obtained from Sinopharm Chemical Reagent Co. Ltd. (China). They were used to prepare saline water as a chemical quenching agent. Nitric acid (guaranteed reagent) was used to prepare carrier solution and acidify samples with received. All aqueous solutions were prepared with deionized water.
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4

Comprehensive Inorganic Chemical Reagents

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Melamine, phenol, formaldehyde solution (37–40 wt%), methanol, HCl, NaOH, NaCl, K2CO3, KNO3, NaNO2, Na2SO4, Na2SO3, Na2S, Na2S2O3, K2Cr2O7, Sodium aceticum (NaAc), KF, KCl, KBr, KI, KMnO4, K3Fe(CN)6, K2FeO4 and Zn(OH)2 were provided by Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Quinine sulfate was purchased from Adamas-beta Inc. (Shanghai, China). All chemicals were of analytical grade and used directly without further purification. Ultrapure water (18.2 MΩ cm−1) used in this study was obtained from a Millipore water purification system.
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5

Melatonin Metabolism Pathway Analysis

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All reagents were of analytical grade unless otherwise stated. Melatonin (MT), 5-hydroxytryptophan (5-HTP), serotonin (5-HT), indole-3-acetamide (IAM), tryptamine (TrA), 3-hydroxyanthranilic acid (3-HAA), niacin, xanthurenic acid (XA), tryptophan (Trp), kynurenine (KYN), kynurenine acid (KA), indole-3-carboxaldehyde (IAId), and indole-3-acetic acid (IAA) were purchased from Sigma-Aldrich (Darmstadt, Germany); the purity of these standards was >99%. Bondesil C 18 sorbent (average particle size 40 μm) was purchased from Agilent Technologies Inc. (Santa Clara, CA), and Na 2 SO 4 and NaCl were purchased from Sinopharm Chemical Reagent Co. Ltd. (Shanghai, China) . Water was purified by using a Milli-Q system from Millipore (Bedford, MA). Formic acid, methanol (MeOH), and acetonitrile (ACN) were HPLC grade and purchased from Fisher Scientific (Pittsburgh, PA).
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6

Synthesis and Evaluation of Antioxidant Nanocomposites

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Zn(NO3)2·6H2O and Na2SO4 were purchased from Sinopharm. Pyrazine was acquired from Alfa Aesar. PVP, Mn-SOD, catalase, complete Freund’s adjuvant, and L-ascorbic acid were acquired from Sigma–Aldrich. MnTCPP+ monochloride, TCPP+ monochloride, FeTCPP+ monochloride, gallic acid and reduced glutathione were provided by J&K Scientific. N,N-dimethylformamide (DMF) was bought from Shanghai Lingfeng Chemical Reagent Co., Ltd. CeO2 nanoparticles were purchased from Jiangsu XFNANO. SOD assay kit, catalase assay kit, Hoechst, LPS, and alizarin red S staining kit were acquired from Beyotime. Dulbecco’s modified eagle’s medium (DMEM), PBS, fetal bovine serum (FBS), and penicillin/streptomycin were obtained from YoBiBio. Annexin V-FITC, PI and CCK-8 assay were provided by 7seabiotech. Mesenchymal stem cell medium was bought from ScienCell. FITC, DCFH-DA and rhodamine phalloidin were provided by Solarbio. Paraformaldehyde (PFA) was obtained from Ribiology.
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7

Sodium Sulfate Hydrogel Electrolytes

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Aqueous electrolyte (AE, 1 M anhydrous sodium sulfate (Na2SO4, Sinopharm Chemical Reagent Co., Ltd) in deionized water (HPLC grade)) was prepared according to molality (mol-salt in kg-solvent). The hydrogel electrolytes (HEs) were prepared by mixing 1 M Na2SO4 solution with different ratios (1 wt%, 3 wt%, 5 wt%, named HE-1, HE-3, HE-5, respectively) of poly(acrylate sodium) (PAAS, Mw = 300w–700w, Aladdin), and then followed by intense stirring under room temperature until dissolved.
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8

Extraction and Purification of ε-PL

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Methanol, ethanol, acetone, ethyl acetate, (NH4)2SO4, NaH2PO4, KCl, NaCl, Na3PO4 and Na2SO4 were purchased from Sinopharm Chemical Reagent Co. Ltd (Shanghai, China). Acetonitrile was obtained from TEDIA (Fairfield, Ohio, USA). A protein assay kit was obtained from Bio-Rad (California, USA). The standard ε-PL sample with a purity of 98.5% was provided by Jiangsu Yiming Biological Co., Ltd (Jiangsu, China). All chemicals used in this study were of analytical grade.
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9

Synthesis of Organic-Inorganic Hybrid Materials

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Urea (H2NCONH2), barium sulfate (BaSO4), anhydrous sodium sulfate (Na2SO4), N,N-dimethylformamide (DMF), and ethanol (C2H6O) were purchased from Sinopharm. APTES (C9H23NO3Si) and TCNQ were purchased from Aladdin. All the materials were used without any further purification.
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10

Moxa Ash-based Sorbent for Mitoxantrone Removal

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Chemical solutions were created using deionized (DI) water (18.2 MΩ). Mitoxantrone hydrochloride (≥97%), 30% H2O2, hydrochloric acid, and sodium hydroxide were obtained from Aladdin Chemistry Co., Ltd. (Shanghai, China). NaCl, Na2CO3, Na2SO4, NaNO3 and humic acid (HA) were purchased from Sinopharm Chemical Reagent Co., Ltd. All reagents used above are analytically pure. Moxa was purchased from Jinan Ou Mai Medical Instrument Co., Ltd.
Moxa was ignited in the air for 5 min until the combustion was completed to obtain moxa ash (MA), and then passed through 100 meshes and rinsed repeatedly with DI water to achieve a constant pH value. In a 500 mL agate jar, 100 g of 3 mm, 5 mm, and 10 mm diameter agate balls were combined with 1 g of MA in a mass ratio of 3 : 5 : 2, with or without addition of H2O or H2O2. The jar was then sealed and put into a planetary ball mill that was rotated in a different direction every 6 h while being driven at 300 rpm for 12 h.28 (link) The resulting samples were labelled as BMMA, BMMA-H2O, and BMMA-H2O2. Fig. 1 shows a schematic process for the production and modification of moxa ash and the difference in their ability to remove MTX.
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