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Biotinylated anti kappa ab

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Biotinylated anti-kappa Ab is a laboratory reagent used for the detection and analysis of kappa light chains in biological samples. It is a biotinylated antibody that specifically binds to kappa light chains, providing a means to identify and quantify their presence.

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Lab products found in correlation

Alkaline phosphatase conjugated anti mouse igg, by Thermo Fisher Scientific (10 mentions) Biotinylated anti mouse igm, by Jackson ImmunoResearch (8 mentions) Streptavidin sa alkaline phosphatase, by Vector Laboratories (8 mentions) Vector blue alkaline phosphatase substrate kit 3, by Vector Laboratories (6 mentions) Anti igm or anti igg, by Thermo Fisher Scientific (4 mentions) Salmon sperm dsdna, by Thermo Fisher Scientific (4 mentions) Histone, by Merck Group (2 mentions) Elisa plate, by Thermo Fisher Scientific (2 mentions) Sa alkaline phosphatase, by Vector Laboratories (2 mentions) Nucleosome, by Thermo Fisher Scientific (2 mentions) Nucleosome, by Merck Group (2 mentions) Smrnp, by Thermo Fisher Scientific (2 mentions) Elispot plate imaging analysis system, by Cellular Technology (2 mentions) Dsdna, by Thermo Fisher Scientific (2 mentions) Calf thymus histone, by Merck Group (2 mentions) Nucleosome coated plates, by Merck Group (2 mentions) Sa alkaline phosphatase or alkaline phosphatase conjugated anti mouse igg, by Thermo Fisher Scientific (2 mentions) Elispot plate imaging and analysis system, by Cellular Technology (2 mentions) Multiscreen 96 well filtration plate, by Merck Group (2 mentions) Computerized imaging analysis system, by Cellular Technology (2 mentions)

10 protocols using biotinylated anti kappa ab

1

ELISA-based Antibody Titer Measurement

Cited 1 time
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Ig and ANA titers were measured by ELISA as previously described (Wong et al., 2012 (link)). Briefly, for IgG or IgM detection, ELISA plates (Thermo Fisher Scientific, Waltham, MA) were coated with anti-IgM or anti-IgG (Invitrogen, Grand Island, NY) capture antibodies and detected using biotinylated antimouse IgM (Jackson ImmunoResearch, West Grove, PA) or alkaline-phosphatase conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Total IgG autoAb titers were measured in plates coated with salmon sperm dsDNA (Invitrogen, Grand Island, NY), histone (Sigma Aldrich, St. Louis, MO), or nucleosome and detected with biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY). IgG subtype-specific autoAb titers were detected by biotinylated-IgG1, -IgG2b, and AP-IgG2c Abs (Southern Biotech, Birmingham, AL). Biotinylated antibodies were detected by SA-alkaline phosphatase (Vector Laboratories, Burlingame, CA). The plates were developed by the PNPP (p-nitrophenyl phosphate, disodium salt; Thermo Fisher Scientific, Rockford, IL) substrates for alkaline phosphatase and read at γ405 nm.
Domeier P.P., Chodisetti S.B., Schell S.L., Kawasawa Y.I., Fasnacht M.J., Soni C, & Rahman Z.S. (2018). B-Cell-Intrinsic Type 1 Interferon Signaling Is Crucial for Loss of Tolerance and the Development of Autoreactive B Cells. Cell reports, 24(2), 406-418.
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2

Quantitative ELISpot Assay for Autoantibodies

Cited 2 times
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ELISpot assays were performed as previously described (5 (link), 6 (link)). Briefly, splenocytes in RPMI containing 10% fetal bovine serum were plated at a concentration of 1 x 106 cells/well onto salmon sperm dsDNA- (Invitrogen), nucleosome- (histone from Sigma Aldrich plated on a layer of dsDNA coating)- or SmRNP- (Arotec Diagnostics) coated multiscreen 96-well filtration plates (Millipore, Bedford, MA). Serially diluted (1:2) cells were incubated for 12 h at 37°C. dsDNA-, nucleosome-, and SmRNP-specific AFCs were detected by biotinylated anti-kappa Ab (Invitrogen) and streptavidin (SA)-alkaline phosphatase (Vector Laboratories) or alkaline phosphatase-conjugated anti-mouse IgG (Molecular Probes). Plates were developed using the Vector Blue Alkaline phosphatase Substrate Kit III (Vector Laboratories). ELISpots were enumerated and analyzed using a computerized ELISpot plate imaging/ analysis system (Cellular Technology).
Chodisetti S.B., Fike A.J., Domeier P.P., Schell S.L., Mockus T.E., Choi N.M., Corradetti C., Hou B., Atkins H.M., Caricchio R., Decker T., Lukacher A.E., Olsen N, & Rahman Z.S. (2020). Serine phosphorylation of the STAT1 transactivation domain promotes autoreactive B cell and systemic autoimmunity development. Journal of immunology (Baltimore, Md. : 1950), 204(10), 2641-2650.
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3

ELISpot Assay for Detecting Autoantibodies

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ELISpot assays were performed as previously described (25 (link), 43 (link)). Briefly, splenocytes in RPMI containing 10% fetal bovine serum were plated at a concentration of 1 × 106 cells/well onto salmon sperm dsDNA- (Invitrogen), nucleosome- (histone from Sigma Aldrich plated on a layer of dsDNA coating)- or SmRNP- (Arotec Diagnostics) coated multiscreen 96-well filtration plates (Millipore, Bedford, MA). Serially diluted (1:2) cells were incubated for 12 h at 37°C. dsDNA-, nucleosome-, and SmRNP-specific AFCs were detected by biotinylated anti-kappa Ab (Invitrogen) and streptavidin (SA)-alkaline phosphatase (Vector Laboratories) or alkaline phosphatase-conjugated anti-mouse IgG (Molecular Probes). Plates were developed using the Vector Blue Alkaline phosphatase Substrate Kit III (Vector Laboratories). ELISpots were enumerated and analyzed using a computerized ELISpot plate imaging/ analysis system (Cellular Technology).
Chodisetti S.B., Fike A.J., Domeier P.P., Singh H., Choi N.M., Corradetti C., Kawasawa Y.I., Cooper T.K., Caricchio R, & Rahman Z.S. (2020). Type II but not type I interferon signaling is indispensable for TLR7-promoted development of autoreactive B cells and systemic autoimmunity. Journal of immunology (Baltimore, Md. : 1950), 204(4), 796-809.
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4

ELISpot Assay for Antibody-Producing Cells

Cited 1 time
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ELISpot assays were performed as described (Wong et al., 2012 (link)). Briefly, splenocytes in RPMI containing 10% fetal bovine serum were plated at a concentration of 1 × 105 cells/well onto anti-IgM-, anti-IgG-, salmon sperm dsDNA- (Invitrogen, Grand Island, NY), calf thymus histone- (Sigma Aldrich, St. Louis, MO), or nucleosome-coated plates (Millipore, Bedford, MA). Serially diluted (1:2) cells were incubated for 6 hr at 37°C. IgM-producing AFCs were detected using biotinylated anti-mouse IgM (Jackson ImmunoResearch, West Grove, PA) and streptavidin (SA)-alkaline phosphatase (Vector Laboratories, Burlingame, CA). IgG-producing AFCs were detected using alkaline phosphatase-conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). dsDNA-, histone-, and nucleosome-specific AFCs were detected by biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY) and SA-alkaline phosphatase or alkaline phosphatase-conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Plates were developed using the Vector Blue Alkaline phosphatase Substrate Kit III (Vector Laboratories, Burlingame, CA). ELISpots were enumerated using a computerized ELISpot plate imaging and analysis system (Cellular Technology, Shaker Heights, OH).
Domeier P.P., Chodisetti S.B., Schell S.L., Kawasawa Y.I., Fasnacht M.J., Soni C, & Rahman Z.S. (2018). B-Cell-Intrinsic Type 1 Interferon Signaling Is Crucial for Loss of Tolerance and the Development of Autoreactive B Cells. Cell reports, 24(2), 406-418.
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5

Quantification of Antibody-Secreting Cells by ELISpot

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ELISpot assays were performed as previously described (16 (link)). Briefly, splenocytes in 10% RPMI were plated at a concentration of 1 × 105 cells/well onto anti-IgM or anti-IgG (Invitrogen, Grand Island, NY) coated, or at 1 × 106 cells/well on dsDNA-, histone-, or nucleosome-coated multiscreen 96-well filtration plates (Millipore, Bedford, MA). Serially diluted (1:2) cells were incubated for 6 h at 37°C. IgM-producing AFCs were detected using biotinylated anti-mouse IgM (Jackson Immunoresearch, West Grove, PA) and streptavidin (SA)-alkaline phosphatase (Vector Laboratories, Burlingame, CA). IgG-producing AFCs were detected using alkaline phosphatase-conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). dsDNA-, histone-, and nucleosome-specific AFCs were detected by biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY) and streptavidin (SA)-alkaline phosphatase (Vector Laboratories, Burlingame, CA) or alkaline phosphatase-conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Plates were developed using the Vector Blue Alkaline phosphatase Substrate Kit III (Vector Laboratories, Burlingame, CA). ELISpots were enumerated and analyzed using a computerized imaging/analysis system (Cellular Technology, Shaker Heights, OH).
Wong E.B., Soni C., Chan A.Y., Domeier P.P., S.h.w.e.t.a.n.k., Abraham T., Limaye N., Khan T.N., Elias M.J., Chodisetti S.B., Wakeland E.K, & Rahman Z.S. (2015). B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance. Journal of immunology (Baltimore, Md. : 1950), 194(9), 4130-4143.
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6

Quantifying Mouse Autoantibody Isotypes by ELISA

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ELISA plates were coated with anti-IgM or anti-IgG capture antibodies (from Invitrogen, Grand Island, NY) and detected using biotinylated anti-mouse IgM (Jackson Immunoresearch, West Grove, PA) or alkaline-phosphatase conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Total IgG autoAb titers were measured in ELISA plates coated with dsDNA, histone, nucleosome, Sm/RNP or cardiolipin and detected with biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY). IgG subtype-specific autoAb titers were detected by biotinylated-IgG1, biotinylated IgG2b, and AP-IgG2c Abs (Southern Biotech, Birmingham, AL). Biotinylated antibodies were detected by streptavidin (SA)-alkaline phosphatase (Vector Laboratories, Burlingame, CA). The plates were developed by the PNPP (p-Nitrophenyl Phosphate, Disodium Salt) (Thermo Fisher Scientific, Rockford, IL) substrates for alkaline phosphatase.
Wong E.B., Soni C., Chan A.Y., Domeier P.P., S.h.w.e.t.a.n.k., Abraham T., Limaye N., Khan T.N., Elias M.J., Chodisetti S.B., Wakeland E.K, & Rahman Z.S. (2015). B cell-intrinsic CD84 and Ly108 maintain germinal center B cell tolerance. Journal of immunology (Baltimore, Md. : 1950), 194(9), 4130-4143.
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7

ELISpot Assay for Antibody-Producing Cells

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
ELISpot assays were performed as described (Wong et al., 2012 (link)). Briefly, splenocytes in RPMI containing 10% fetal bovine serum were plated at a concentration of 1 × 105 cells/well onto anti-IgM-, anti-IgG-, salmon sperm dsDNA- (Invitrogen, Grand Island, NY), calf thymus histone- (Sigma Aldrich, St. Louis, MO), or nucleosome-coated plates (Millipore, Bedford, MA). Serially diluted (1:2) cells were incubated for 6 hr at 37°C. IgM-producing AFCs were detected using biotinylated anti-mouse IgM (Jackson ImmunoResearch, West Grove, PA) and streptavidin (SA)-alkaline phosphatase (Vector Laboratories, Burlingame, CA). IgG-producing AFCs were detected using alkaline phosphatase-conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). dsDNA-, histone-, and nucleosome-specific AFCs were detected by biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY) and SA-alkaline phosphatase or alkaline phosphatase-conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Plates were developed using the Vector Blue Alkaline phosphatase Substrate Kit III (Vector Laboratories, Burlingame, CA). ELISpots were enumerated using a computerized ELISpot plate imaging and analysis system (Cellular Technology, Shaker Heights, OH).
Domeier P.P., Chodisetti S.B., Schell S.L., Kawasawa Y.I., Fasnacht M.J., Soni C, & Rahman Z.S. (2018). B-Cell-Intrinsic Type 1 Interferon Signaling Is Crucial for Loss of Tolerance and the Development of Autoreactive B Cells. Cell reports, 24(2), 406-418.
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8

ELISA-based Antibody Titer Measurement

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Ig and ANA titers were measured by ELISA as previously described (Wong et al., 2012 (link)). Briefly, for IgG or IgM detection, ELISA plates (Thermo Fisher Scientific, Waltham, MA) were coated with anti-IgM or anti-IgG (Invitrogen, Grand Island, NY) capture antibodies and detected using biotinylated antimouse IgM (Jackson ImmunoResearch, West Grove, PA) or alkaline-phosphatase conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Total IgG autoAb titers were measured in plates coated with salmon sperm dsDNA (Invitrogen, Grand Island, NY), histone (Sigma Aldrich, St. Louis, MO), or nucleosome and detected with biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY). IgG subtype-specific autoAb titers were detected by biotinylated-IgG1, -IgG2b, and AP-IgG2c Abs (Southern Biotech, Birmingham, AL). Biotinylated antibodies were detected by SA-alkaline phosphatase (Vector Laboratories, Burlingame, CA). The plates were developed by the PNPP (p-nitrophenyl phosphate, disodium salt; Thermo Fisher Scientific, Rockford, IL) substrates for alkaline phosphatase and read at γ405 nm.
Domeier P.P., Chodisetti S.B., Schell S.L., Kawasawa Y.I., Fasnacht M.J., Soni C, & Rahman Z.S. (2018). B-Cell-Intrinsic Type 1 Interferon Signaling Is Crucial for Loss of Tolerance and the Development of Autoreactive B Cells. Cell reports, 24(2), 406-418.
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9

Quantification of serum IgM/IgG and autoantibodies

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Total serum IgM/IgG or IgG from in vitro B cell culture supernatants was measured using standard ELISA protocols. Serum Ab titers were quantitated as described (29 ). Briefly, ELISA plates were coated with anti-IgM or anti-IgG capture antibodies (from Invitrogen, Grand Island, NY) and detected using biotinylated anti-mouse IgM (Jackson Immunoresearch, West Grove, PA) or alkaline-phosphatase conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). Total IgG autoAb titers were measured in ELISA plates coated with dsDNA, histone, nucleosome, Sm/RNP or cardiolipin and detected with biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY). IgG subtype-specific autoAb titers were detected by biotinylated-IgG1, biotinylated IgG2b, and AP-IgG2c Abs (Southern Biotech, Birmingham, AL). Biotinylated antibodies were detected by streptavidin (SA)-alkaline phosphatase (Vector Laboratories, Burlingame, CA). The plates were developed by the PNPP (p-Nitrophenyl Phosphate, Disodium Salt) (Thermo Fisher Scientific, Rockford, IL) substrates for alkaline phosphatase.
Soni C., Wong E.B., Domeier P.P., Khan T.N., Satoh T., Akira S, & Rahman Z.S. (2014). B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers. Journal of immunology (Baltimore, Md. : 1950), 193(9), 4400-4414.
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10

Quantifying Antibody-Producing Cells via ELISpot

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ELISpot assays were performed as described (37 (link)). Briefly, splenocytes in 10% RPMI with antibiotics were plated at a concentration of 1 × 105 cells/well onto anti-IgM or anti-IgG (Invitrogen, Grand Island, NY) coated, or at 1 × 106 cells/ well on dsDNA-, histone-, or nucleosome-coated multiscreen 96-well filtration plates (Millipore, Bedford, MA). Serially diluted (1:2) cells were incubated for 6 h at 37°C. IgM-producing AFCs were detected using biotinylated anti-mouse IgM (Jackson Immunoresearch, West Grove, PA) and streptavidin (SA)-alkaline phosphatase (Vector Laboratories, Burlingame, CA). IgG-producing AFCs were detected using alkaline-phosphatase conjugated anti-mouse IgG (Molecular Probes, Grand Island, NY). dsDNA-, histone-, and nucleosome-specific AFCs were detected by biotinylated anti-kappa Ab (Invitrogen, Grand Island, NY) and streptavidin (SA)-alkaline phosphatase (Vector Laboratories, Burlingame, CA). Plates were developed using the Vector Blue Alkaline-phosphatase Substrate Kit III (Vector Laboratories, Burlingame, CA). ELISpots were counted and analyzed using a computerized imaging/ analysis system (Cellular Technology, Shaker Heights, OH).
Soni C., Wong E.B., Domeier P.P., Khan T.N., Satoh T., Akira S, & Rahman Z.S. (2014). B cell-intrinsic TLR7 signaling is essential for the development of spontaneous germinal centers. Journal of immunology (Baltimore, Md. : 1950), 193(9), 4400-4414.
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