Gc 2010 plus gas chromatograph

Manufactured by Shimadzu

Sourced in Japan

The GC-2010 Plus is a gas chromatograph manufactured by Shimadzu. It is a compact and high-performance instrument designed for the analysis of volatile organic compounds. The GC-2010 Plus features a high-precision temperature control system and advanced data processing capabilities.

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Lab products found in correlation

Cpsil 88 fused silica capillary column, by Agilent Technologies (3 mentions) Fame 37, by Merck Group (3 mentions) 2010 plus single quadrupole mass spectrometer, by Shimadzu (3 mentions) Aoc 20s autosampler, by Shimadzu (3 mentions) Fame 189 19, by Merck Group (3 mentions) Hp ffap column, by Agilent Technologies (2 mentions) Tr wax capillary column, by Teknokroma (2 mentions) Qp2010 plus mass spectrometer, by Shimadzu (2 mentions) Aoc 20i autoinjector, by Shimadzu (2 mentions) Cp sil 8 cb fused silica capillary gc column, by Agilent Technologies (2 mentions) Qp2010se mass spectrometer, by Shimadzu (2 mentions) Gcms qp2010 ultra system, by Shimadzu (1 mentions) Rxi 5ms capillary column, by Restek (1 mentions) Tqms 8040 triple quadrupole mass spectrometer, by Shimadzu (1 mentions) 0.2 m filter, by Merck Group (1 mentions) Quantofix test strips, by Macherey-Nagel (1 mentions) Gcms tq8030, by Shimadzu (1 mentions) Db wax column, by Shimadzu (1 mentions) Clark type electrode, by Hansatech (1 mentions) Oxytherm, by Hansatech (1 mentions)

50 protocols using gc 2010 plus gas chromatograph

Electrochemical CO2 Reduction Protocol

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Long-term
PEC measurements
were carried out in a sealed two-compartment cell (V_solution = 40 cm³) where the compartments were
separated by a Nafion117 membrane. The working electrode was a PEDOT
or PEDOT/nanocarbon modified GC (A = 4 cm²), a Pt sheet was applied as a counter and a Ag/AgCl/3 M NaCl as
a reference electrode while the applied potential was −0.9
V during the measurements. The electrodes were pretreated at the same
potential for 300 s without illumination. The 0.1 M Na₂SO₄ electrolyte was saturated with Ar prior to the measurements
(constant bubbling for 30 min). During the reaction, gas samples were
taken at 30, 60, 90, and 120 min via an online detection system, which
was coupled to the cathode compartment of the cell. Products in the
gas phase were separated with a ShinCarbon ST column and analyzed
with a Shimadzu GC-2010 Plus gas chromatograph equipped with a barrier
discharge ionization (BID) detector. The optimized parameters were
the following: carrier gas, helium; oven program, T_start = 35 °C (2.5 min), ΔT_ramp = 20 °C min^–1, T_end = 270 °C (3 min); injection temperature T = 150 °C; linear velocity was controlled by the pressure p_start = 250 kPa (2.5 min), Δp_ramp = 15 kPa min^–1, p_end = 400 kPa (7.5 min); and the split ratio was 10.

Kormányos A., Hursán D, & Janáky C. (2018). Photoelectrochemical Behavior of PEDOT/Nanocarbon Electrodes: Fundamentals and Structure–Property Relationships. The Journal of Physical Chemistry. C, Nanomaterials and Interfaces, 122(25), 13682-13690.

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Rumen Volatile Fatty Acid Analysis

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Rumen fluid samples were analysed at the Nutrition Laboratory, Massey University, Palmerston North, New Zealand. Volatile fatty acid (VFA) content of rumen fluid collected at slaughter was analysed by gas chromatography as described by Sukhija and Palmquist [18 (link)]. Briefly, rumen fluid samples were suspended in toluene and fatty acids were methylated by using methanolic hydrochloride (a mixture of methanol and acetyl chloride) in culture tubes. Samples were vortexed and heated at 70 °C for methylation for 2 h. After methylation, samples were cooled on ice and potassium carbonate and toluene were added. Samples were vortexed and centrifuged at 2500 rpm for 7 min at room temperature to separate the solvent layer containing methyl esters and the aqueous layer. The VFA content was determined by Shimadzu GC-2010 Plus Gas Chromatograph (Shimadzu, Kyoto, Japan) equipped with a Supelco^TM-2560 Capillary Column (100 m × 0.25 mm × 0.2 μm film thickness). The acetic, propionic, n-butyric, iso-butyric, iso-valeric, n-valeric, and n-caproic acid contents were determined.

Herath H.M., Pain S.J., Kenyon P.R., Blair H.T, & Morel P.C. (2021). Rumen Development of Artificially-Reared Lambs Exposed to Three Different Rearing Regimens. Animals : an Open Access Journal from MDPI, 11(12), 3606.

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Essential Oil Composition Analysis

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The analysis of the essential oil was carried out using a GC/MS-QP 2010 Ultra System (Shimadzu, Kyoto, Japan) and a Shimadzu GC-2010 Plus gas chromatograph. A Rxi-5MS capillary column (30 m × 0.25 mm, 0.25 μm film thickness, Restek, Bellefonte, USA) was used. Helium was the carrier gas at 1 mL/min with following temperature program: at 60 °C for 1 min, increased to 280 °C at the rate of 3 °C/min held for 5 min. Samples were injected at a temperature of 250 °C with a split ratio of 1/40 during 1 min. An electron impact ionization system with ionization energy of 70 eV and electron ionization spectra with a mass scan range of 30–500 m/z were used.

Huang J., Qian C., Xu H, & Huang Y. (2017). Antibacterial activity of Artemisia asiatica essential oil against some common respiratory infection causing bacterial strains and its mechanism of action in Haemophilus influenzae. Microbial Pathogenesis, 114, 470-475.

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Fatty Acid Profiling and Cardiovascular Risk Indices

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The fatty acid profile was obtained by gas chromatography in a Shimadzu GC-2010 Plus Gas Chromatograph (Shimadzu, Tokyo, Japan) using a CPSil 88 fused silica capillary column (50 m × 0.25 mm i.d.), 0.20 m film thickness (Varian, Middelburg, The Netherlands), and helium as the carrier gas (120 kPa). Each fatty acid methyl ester (FAME) was identified by direct comparison with a standard mixture (FAME 37, Supelco, Bellefonte, PA, USA) [27 (link)]. Two samples of each batch were analyzed after the extraction of the fat, and the results are expressed as the percentage of each FAME. On the other hand, Atherogenicity (AI) was calculated from the percentage of lauric (C12:0), myristic (C14:0), and pamitic (C16:0) acids, and the sum of the polyunsaturated (PUFA) and monounsaturated (MUFA) fatty acids. On the other hand, the Thrombogenicity index (TI) was calculated by using the percentage of myristic, palmitic, and stearic (C18:0) acids and the sums of MUFA, omega 6 (n = 6) and the ratio between omega-6 and omega-3 (n = 3).

AI = \frac{C 12 : 0 + (4 * C 14 : 0 + C 16 : 0)}{\sum^{} PUFA + \sum^{} MUFA}

TI = \frac{C 14 : 0 + C 16 : 0 + C 18 : 0}{0.5 * \sum^{} MUFA + 0.5 \sum^{} (n = 6) + 3 \sum^{} (n = 3) + \frac{\sum^{} (n = 3)}{\sum^{} (n = 6)}}

Martínez E., Pardo J.E., Álvarez-Ortí M., Rabadán A., Pardo-Giménez A, & Alvarruiz A. (2023). Substitution of Pork Fat by Emulsified Seed Oils in Fresh Deer Sausage (‘Chorizo’) and Its Impact on the Physical, Nutritional, and Sensory Properties. Foods, 12(4), 828.

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Fatty Acid Profile Analysis in Burgers

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With regards to fatty acid profile, first fat was extracted from burgers with a chloroform-methanol mixture (2:1) according to [36 (link)]. Then, fatty acid methyl esters (FAME) were obtained by a transmethylation according to ISO 12988-2:2017 [37 ]. Then, FAMEs were injected in a Shimadzu GC-2010 Plus Gas Chromatograph (Shimadzu, Tokyo, Japan), equipped with a CPSil 88-fused silica capillary column (50 m × 0.25 mm i.d.), 0.20 m film thickness (Varian, Middelburg, Netherlands), using helium as the carrier gas (120 kPa). Each fatty acid methyl ester (FAME) was identified by direct comparison with a standard mixture (FAME 37, Supelco, Bellefonte, PA, USA). Two samples of each batch were analyzed, and the results were expressed as the percentage of each FAME.

Martínez E., Pardo J.E., Rabadán A, & Álvarez-Ortí M. (2023). Effects of Animal Fat Replacement by Emulsified Melon and Pumpkin Seed Oils in Deer Burgers. Foods, 12(6), 1279.

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Analyzing Short-Chain Fatty Acids by GC

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Analysis of SCFA by GC was carried out following a previously published method by Richardson, et al. [20 (link)]. The analysis was carried out using a Shimadzu GC-2010 Plus gas chromatograph (Shimadzu, Kyoto, Japan) with a barrier ionization detector (helium ionization) and a 30 m × 0.53 mm I.D. × 50 μm film MXT-Msieve5A PLOT capillary column (Restek Corporation, Bellefonte, PA, USA). A split injection of a 1 µL sample was made at a ratio of 5:1, with a column helium flow rate of 1.36 mL/min (total flow: 11.2 mL/min). Injector and detector temperatures were both 240 °C. The column temperature was initially held at 50 °C for 2 min and then increased by 5 °C/min to 130 °C, followed by 15 °C/min to 240 °C (held for 4.7 min). Run time per sample was 30 min. Following this, the resulting data were analyzed using a two-way permutation ANOVA in R [21 ], with time and treatment as factors. Permutation ANOVA is a non-parametric test that does not require data to be normally distributed.

Ahlborn N., Young W., Mullaney J, & Samuelsson L.M. (2020). In Vitro Fermentation of Sheep and Cow Milk Using Infant Fecal Bacteria. Nutrients, 12(6), 1802.

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Fatty Acid Profiling of Plant Samples

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Ten milligrams of dry seeds and two leaf samples were transmethylated at 85°C for 90 min in 0.5 ml toluene and 0.5 ml 5% (v/v) H₂SO₄ in methanol. Heptadecanoic acid (17:0) was added to each sample as an internal standard to measure the amount of FAs. After transmethylation, 1.5 ml 0.9% (w/v) NaCl solution was added, and fatty acid methyl esters (FAMEs) were extracted with 0.5 ml n‐hexane. FAMEs were analyzed using gas chromatography (GC) on a GC‐2010 Plus Gas Chromatograph (Shimadzu, Japan) with a 30 m × 0.25 mm (inner diameter) HP‐FFAP column (Agilent, USA) while the oven temperature was increased from 190°C to 230°C at 3°C min⁻¹. Nitrogen was used as the carrier gas at a flow rate of 1.4 ml min⁻¹.

Kim I., Lee K., Park M, & Kim H.U. (2022). The seed‐specific transcription factor DPBF2 modulates the fatty acid composition in seeds. Plant Direct, 6(4), e395.

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GC-MS Analysis of Volatile Compounds

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For the analysis, a Shimadzu GC 2010 Plus gas chromatograph directly interfaced with a TQMS 8040 triple quadrupole mass spectrometer (Shimadzu, Milan, Italy) was used. The conditions were as follows: injector temperature, 260 °C; injection mode, splitless; capillary column, VF-WAXms, 60 m × 0.25 mm i.d. × 0.25 μm film thickness (Agilent, S.p.a. Milan, Italy); oven temperature, 45 °C held for 5 min, then increased to 80 °C at a rate of 10 °C/min and to 240 °C at 2 °C/min; carrier gas, helium at a constant flow of 1 mL/min; transfer line temperature, 250 °C; acquisition range, 30 to 360 m/z; scan speed, 1250 amu/s. The volatile compounds were identified using mass spectral data, NIST’ 18 (NIST/ EPA/NIH Mass Spectra Library, version 2.0, Gaithersburg, MD, USA) and the FFNSC 3.0 database, linear retention indices (LRI), literature data, and the injection of standards were available, as previously reported by Cincotta et al. [23 (link)].

Conte F., Cincotta F., Condurso C., Verzera A, & Panebianco A. (2021). Odor Emissions from Raw Meat of Freshly Slaughtered Cattle during Inspection. Foods, 10(10), 2411.

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Fatty Acid Profiling of Porcine Muscle

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Approximately 50 g of longissimus dorsi tissue was dissected from the 3rd to 4th lumbar vertebrae region of each pig and frozen in liquid nitrogen within 30 min post-mortem, and then stored at −80 °C for further use. About 10 g of longissimus dorsi tissue was ground and then treated with a 3:1 chloroform–methanol solution according to [12 (link)]. Then, 2 mg of extracted lipids was re-dissolved in 2 mL of n-hexane and 1 mL of KOH (0.4 M) for saponification and methylation. Fatty acid methyl esters were processed with a GC2010 Plus Gas Chromatograph (Shimadzu), following manufacturer recommendations. Signals for each fatty acid were quantified relative to standard reference reagents (Sigma-Aldrich) and the percentage of each fatty acid relative to total fatty acids was used as phenotype for further analyses. The same measurement procedures were used for all five populations.

Zhang W., Zhang J., Cui L., Ma J., Chen C., Ai H., Xie X., Li L., Xiao S., Huang L., Ren J, & Yang B. (2016). Genetic architecture of fatty acid composition in the longissimusdorsi muscle revealed by genome-wide association studies on diverse pig populations. Genetics, Selection, Evolution : GSE, 48, 5.

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Multi-Analyte Water Quality Assessment

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For chemical analysis, the water samples were filtered through 0.2 µm filters (Merck Millipore, Darmstadt, Germany) and analyzed with ICP-MS and ion chromatography, as reported previously [44 (link)]. Gas composition in the grown cultures was determined using a Kristall-5000.1 gas chromatograph (Chromatec, Yoshkar-Ola, Mari El, Russia) equipped with a katharometer detector. Volatile fatty acids were analyzed using a GC-2010 Plus gas chromatograph (Shimadzu, Kyoto, Japan). The concentration of nitrate ions was determined using an Expert-001 ionometer (Econix-Expert, Moscow, Russia), as described previously [45 (link)]. Nitrite was determined using Quantofix test strips (Macherey-Nagel, Düren, Germany). Sulfide was monitored with the colorimetrical method by Trüper and Schlegel [46 (link)].

Kadnikov V.V., Ravin N.V., Sokolova D.S., Semenova E.M., Bidzhieva S.K., Beletsky A.V., Ershov A.P., Babich T.L., Khisametdinov M.R., Mardanov A.V, & Nazina T.N. (2023). Metagenomic and Culture-Based Analyses of Microbial Communities from Petroleum Reservoirs with High-Salinity Formation Water, and Their Biotechnological Potential. Biology, 12(10), 1300.

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