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Psb1115

Manufactured by Bio-Techne
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Sourced in United Kingdom, United States

The PSB1115 is a versatile lab equipment designed for various applications. It serves as a power supply unit, providing a stable and reliable source of electrical power for laboratory instruments and equipment.

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Lab products found in correlation

Zm241385, by Bio-Techne (2 mentions) Bay 60 6583, by Bio-Techne (1 mentions) αβ methylene adp, by Merck Group (1 mentions) 5 deoxycoformycin, by Merck Group (1 mentions) Cgs21680, by Bio-Techne (1 mentions) Ac yvad cmk, by Merck Group (1 mentions) Sgp130fc, by R&D Systems (1 mentions) E coli serotype o55 b5, by Merck Group (1 mentions) Roswell park memorial institute (rpmi), by Merck Group (1 mentions) Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions) Wortmannin, by Cell Signaling Technology (1 mentions) Psb12379, by Bio-Techne (1 mentions) U0126, by Cell Signaling Technology (1 mentions) Nigericin, by Merck Group (1 mentions) Adenosine, by Merck Group (1 mentions) Tlr ligands, by InvivoGen (1 mentions) Mre 3008f20, by Bio-Techne (1 mentions) Sch442416, by Bio-Techne (1 mentions) C57bl 6 mice, by Jackson ImmunoResearch (1 mentions)

7 protocols using psb1115

1

KPC Tumor Implantation and PSB1115 Treatment

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PSB1115 was purchased from Tocris. C57BL/6 or CD8KO mice (Jax) were implanted with 100K KPC cells subcutaneously and treated with PSB1115 at a concentration of 1 mg/kg for 5 consecutive days per week throughout the duration of the experiment. Vehicle was PBS.
Faraoni E.Y., Singh K., Chandra V., Le Roux O., Dai Y., Sahin I., O'Brien B.J., Strickland L.N., Li L., Vucic E., Warner A.N., Pruski M., Clark T., Van Buren G., Thosani N.C., Bynon J.S., Wray C.J., Bar-Sagi D., Poulsen K.L., Vornik L.A., Savage M.I., Sei S., Mohammed A., Zhao Z., Brown P.H., Mills T., Eltzschig H.K., McAllister F, & Bailey-Lundberg J.M. (2023). CD73-Dependent Adenosine Signaling through Adora2b Drives Immunosuppression in Ductal Pancreatic Cancer. Cancer Research, 83(7), 1111-1127.
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2

Evaluating Anti-Tumor Effects of PSB1115

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PSB1115 was purchased from Tocris. C57BL/6 or CD8KO mice (Jax) were implanted with 100K KPC cells subcutaneously and treated with PSB1115 at a concentration of 1mg/kg for 5 consecutive days per week throughout the duration of the experiment. Vehicle was PBS.
Faraoni E.Y., Singh K., Chandra V., Le Roux O., Dai Y., Sahin I., O'Brien B.J., Strickland L.N., Li L., Vucic E., Warner A.N., Pruski M., Clark T., Van Buren G., Thosani N.C., Bynon J.S., Wray C.J., Bar-Sagi D., Poulsen K.L., Vornik L.A., Savage M.I., Sei S., Mohammed A., Zhao Z., Brown P.H., Mills T., Eltzschig H.K., McAllister F, & Bailey-Lundberg J.M. (2023). CD73-Dependent Adenosine Signaling through Adora2b Drives Immunosuppression in Ductal Pancreatic Cancer. Cancer Research, 83(7), 1111-1127.
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3

Binding Assays for Adenosine Receptors

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PSB1115 (4-(2,3,6,7-Tetrahydro-2,6-dioxo-1-propyl-1H-purin-8-yl)-benzenesulfonic acid), ZM241385 (4-(2-[7-Amino-2-(2-furyl)[1,2,4]triazolo[2,3-a][1,3,5]triazin-5-ylamino]ethyl)phenol), BAY60–6583 (2-[[6-Amino-3,5-dicyano-4-[4-(cyclopropylmethoxy)phenyl]-2-pyridinyl]thio]-acetamide), and CGS21680 (4-[2-[[6-Amino-9-(N-ethyl-β-D-ribofuranuronamidosyl)-9H-purin-2-yl]amino]ethyl]benzenepropanoic acid hydrochloride) were purchased from Tocris Bioscience (Minneapolis, MN). Nitrobenzylthioinosine (NBTI), dipyridamole, αβ-methylene ADP, and 5’-deoxycoformycin and Ac-YVAD-cmk were purchased from Sigma Aldrich (St. Louis, MO).
Chambers E.D., White A., Vang A., Wang Z., Ayala A., Weng T., Blackburn M., Choudhary G., Rounds S, & Lu Q. (2019). Blockade of equilibrative nucleoside transporter 1/2 protects against pseudomonas aeruginosa-induced acute lung injury and NLRP3 inflammasome activation. FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 34(1), 1516-1531.
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4

Adenosine Receptor Antagonist Therapy for ADA Deficiency

Cited 1 time
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PEG-ADA was generated by the covalent modification of purified bovine ADA with activated PEG as described previously (Blackburn et al., 2000 (link)). Ada−/− mice were maintained on high-dose (HD) enzyme therapy at 5U/wk until 8 wks to maintain viability (Mi et al., 2008 (link)). The Ada−/− mice were divided into five groups, each receiving a specific adenosine receptor antagonist, except for controls, that received PBS. For IL-6 antibody studies, mice were divided into two groups. The first was injected IP with neutralizing IL-6 antibody or sgp130Fc (100 μg per day; R&D Systems) for 2 weeks (Le et al., 2014 (link)). The second group was injected daily with PBS or IgG Fc for 2 weeks. SCD mice at 8–14 weeks of age were divided into two groups, followed the same procedures for Ada−/− mice, except for the lower dosage of PEG-ADA (2.5U per week), PSB1115 (200 μg per day; Tocris Bioscience) or sgp130Fc (100 μg per day) for 2 weeks.
Hu X., Adebiyi M.G., Luo J., Sun K., Le T.T., Zhang Y., Wu H., Zhao S., Karmouty-Quintana H., Liu H., Huang A., Wen Y.E., Zaika O.L., Mamenko M., Pochynyuk O.M., Kellems R.E., Etslzchig H.K., Blackburn M.R., Walters E.T., Huang D., Hu H, & Xia Y. (2016). Sustained elevated adenosine via ADORA2B promotes chronic pain through neuro-immune interaction. Cell reports, 16(1), 106-119.
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5

Endotoxin-induced cytokine release assay

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After approval from the local ethics committee of the Radboud university medical center (CMO 2010/10), lithium heparin-anticoagulated blood was obtained from 8 healthy volunteers who provided written informed consent. Blood was diluted 5 times in culture medium (RPMI [Invitrogen, Carlsbad, California, USA] supplemented with 10 μg/mL gentamicin, 10 mM Glutamax and 10 mM pyruvate) and incubated in 48-well plates in the presence of 1 or 10 μM PSB1115 (Tocris BioScience, Abingdon, UK) or vehicle (DMSO, final concentration of 0.1% in all experimental conditions) for 30 min at 37 °C and 5% CO2. Hereafter, 10 μM 5′-N-Ethylcarboxamidoadenosine (NECA) or vehicle (DMSO) was added and cultures were again incubated for 30 min. Subsequently, 10 ng/mL endotoxin (E. coli, serotype O55:B5, Sigma Aldrich) or vehicle (RPMI) was added and cultures were incubated for 24 h, after which plates were centrifuged for 8 min at 1400 RPM at room temperature and supernatant collected and stored at −80 °C until analysis. Cytokine concentrations were measured using ELISA according to the manufacturer's instructions (Human Duoset, R&D systems).
Kiers D., Wielockx B., Peters E., van Eijk L.T., Gerretsen J., John A., Janssen E., Groeneveld R., Peters M., Damen L., Meneses A.M., Krüger A., Langereis J.D., Zomer A.L., Blackburn M.R., Joosten L.A., Netea M.G., Riksen N.P., van der Hoeven J.G., Scheffer G.J., Eltzschig H.K., Pickkers P, & Kox M. (2018). Short-Term Hypoxia Dampens Inflammation in vivo via Enhanced Adenosine Release and Adenosine 2B Receptor Stimulation. EBioMedicine, 33, 144-156.
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6

Modulation of Macrophage Polarization by MSC Exosomes

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To investigate the modulatory effects of MSC exosomes on macrophage polarization, the rat primary macrophages were treated with either 10 μg/mL exosomes or PBS vehicle for 24 and 48 h (Figure 1A). To investigate the role of CD73 in mediating the exosome effects, macrophages were co-treated with 10 μg/mL exosomes and 10 nM PSB12379 (CD73 inhibitor; Tocris Bioscience, Bristol, UK) (Figure 1B). The role of adenosine receptors in the activation of AKT and ERK pathways was investigated by pre-treating the cells with 2 μM of ZM241385 (A2A receptor antagonist; Tocris), 200 nM PSB1115 (A2B receptor antagonist; Tocris), 1 μM wortmannin (AKT inhibitor; Cell Signaling Technology, Danvers, MA, USA), or 10 μM U0126 (ERK inhibitor; Cell Signaling Technology) for 1 h, before treatment with 10 μg/mL exosomes (Figure 1C). For the inhibitor studies, the macrophages were harvested at 30 min for western blot analysis, 24 and 48 h for gene expression analysis, and 48 h for immunofluorescence staining (Figure 1B,C). All in vitro experiments were performed in triplicates (n = 3) in two independent trials.
Teo K.Y., Zhang S., Loh J.T., Lai R.C., Hey H.W., Lam K.P., Lim S.K, & Toh W.S. (2023). Mesenchymal Stromal Cell Exosomes Mediate M2-like Macrophage Polarization through CD73/Ecto-5′-Nucleotidase Activity. Pharmaceutics, 15(5), 1489.
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7

Optimizing Inflammatory Cytokine Assays

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All reagents were from Thermo Fisher Scientific (Waltham, MA) unless otherwise noted. Nigericin, adenosine and MTA were from Sigma-Aldrich (St Louis, MO), while SCH442416, PSB1115 and MRE 3008F20 were from Tocris (Minneapolis, MN) and TLR ligands were from Invivogen (San Diego, CA). Anti-HMGB1 rabbit monoclonal antibody (mAb) EPR3507 was from Genetex (Irvine, CA), anti-actin mouse mAb AC-15 was from Sigma-Aldrich, anti-IL-1β mouse mAb 3ZD was from Frederick National Laboratory for Cancer Research (Frederick, MD), IL-1β ELISA antibodies were from eBioscience (San Diego, CA), TNFα, IL-6 and IL-10 ELISA antibodies were from BioLegend (San Diego, CA), FITC-conjugated anti-CD69 and APC-conjugated anti-CD86 mAb were from BD Biosciences (San Jose, CA), and anti-mouse and anti-rabbit antibodies conjugated to HRP were from Jackson Immunoresearch (West Grove, PA). Bone marrow was either a generous gift from Lisa Borghesi or obtained from C57BL/6 mice (Jackson Labs, Bar Harbor, ME) maintained in accordance with the Texas Tech Institutional Animal Care and Use Committee.
Keyel P.A., Romero M., Wu W., Kwak D.H., Zhu Q., Liu X, & Salter R.D. (2014). Methylthioadenosine Reprograms Macrophage Activation through Adenosine Receptor Stimulation. PLoS ONE, 9(8), e104210.
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