Rabbit and mouse igg isotype controls

Mouse monoclonal anti‐Ki‐67 (Abcam, Cambridge, UK, Cat. No. 15580), rabbit anti‐laminin‐1α (Abcam, Cat. No. 11575), rabbit anti‐IL‐22 (Abcam, Cat. No. 18499), anti‐matrix metalloproteinase (MMP)‐3 (Invitrogen, Carlsbad, CA, USA, Cat. No. MA5‐17123), GAPDH (Cell Signaling, Danvers, MA, USA, Cat. No. 2118s), anti‐CD326‐APC (Biolegend, San Diego, CA, USA, Cat. No. 118214), anti‐CD45‐BV421 (Biolegend, Cat. No. 103134), and rabbit and mouse IgG isotype controls (Sigma, St. Louis, MO, USA). EnVision + Dual Link System‐HRP polymer was purchased from Agilent (Santa Clara, CA, USA). Permount from Fisher Scientific (Hampton, NH, USA) was used as a mounting medium. Carmine Alum was purchased from StemCell Technologies (Vancouver, Canada).

Monoclonal anti‐a2V Ab was generated as described previously (Derks and Beaman, 2004). Rabbit polyclonal anti‐CK14 Ab, anti‐TNF‐α, anti‐collagen IV Ab, anti‐laminin1 + 2 Ab, anti‐GL25D2 Ab, Mu monoclonal anti‐EEA‐1 Ab and mouse anti‐β‐actin Ab were purchased from Abcam (Cambridge, MA, USA); rabbit anti‐GAPDH was from Cell Signaling (Danvers, MA, USA); and rabbit polyclonal anti‐giantin Ab from Biolegend (San Diego, CA, USA). Rabbit and mouse IgG isotype controls were purchased from Sigma (St Louis, MO, USA). EnVision+Dual Link System‐HRP polymer was obtained from DAKO (Glostrup Municipality, Denmark) and Alexa Fluor^® 488 and/or Alexa Fluor^® 594‐conjugated secondary antibody was purchased from Invitrogen. Permount from Fisher scientific and ProLong^® Gold containing DAPI (Invitrogen, Grand Island, NY, USA) were used as mounting media.