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C myc n 262

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C-Myc (N-262) is a rabbit polyclonal antibody that recognizes the N-terminus of the c-Myc protein. c-Myc is a transcription factor that plays a role in cell proliferation, growth, and apoptosis. This antibody can be used for various applications, including Western blotting, immunoprecipitation, and immunohistochemistry.

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Lab products found in correlation

Hif 1α nb100 134, by Novus Biologicals (2 mentions) Bcl 6 c 19, by Santa Cruz Biotechnology (2 mentions) H3k9ac, by Abcam (2 mentions) Actin, by Santa Cruz Biotechnology (1 mentions) Gata4, by Santa Cruz Biotechnology (1 mentions) Proliferating cell nuclear antigen (pcna), by BD (1 mentions) Pro spc, by Santa Cruz Biotechnology (1 mentions) Ha tag clone 3f10, by Roche (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Ctbp2, by BD (1 mentions) Cellytic buffer, by Merck Group (1 mentions) Nupage, by Thermo Fisher Scientific (1 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Thermo Fisher Scientific (1 mentions) Gna13, by Abcam (1 mentions) β actin, by Cell Signaling Technology (1 mentions) Pvdf membrane, by Thermo Fisher Scientific (1 mentions) Protease inhibitor cocktail, by Roche (1 mentions) Ar441, by Santa Cruz Biotechnology (1 mentions) Ar n 20, by Santa Cruz Biotechnology (1 mentions) Enhanced chemiluminescence detection system, by Merck Group (1 mentions)

7 protocols using c myc n 262

1

Immunohistochemistry and Immunofluorescence Staining Protocol

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For immunohistochemistry and immunoflu-orescence staining, primary antibodies against the following proteins were used: Rabbit anti DsRed monoclonal (Clonetech), DsRed (L-18 Santa Cruz), CC10 (S-20 Santa Cruz), c-MYC (N-262 Santa Cruz), GATA-4 (C-20 Santacruz), PGP 9.5 (78-504 AbD Serotec), PCNA (555566 Becton Dickinson Co), pro SPC (Gift from J. Whitsett), Actin (I-19 Santacruz), Aquaporin 5 (Alomene Aqp-005),C-RAF (E 10) Santa Cruz), HA Tag (clone 3F10 Roche), Cleaved Caspase 3 (Cell Signaling).
Thakur C., Rapp U.R, & Rudel T. (2017). Cysts mark the early stage of metastatic tumor development in non-small cell lung cancer. Oncotarget, 9(5), 6518-6535.
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2

Chromatin Immunoprecipitation (ChIP) Assay Protocol

Cited 1 time
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Chromatin Immunoprecipitation (ChIP) assays were carried out as previously described [53 (link)]. Antibodies used for ChIP were BRCA1 (Ab4) from Calbiochem (Darmstadt, Germany) and c-Myc (N-262) from Santa Cruz Technology (CA, USA). ChIP primer sequences are listed in Supplementary Table S3.
Orr K., Buckley N.E., Haddock P., James C., Parent J.L., McQuaid S, & Mullan P.B. (2016). Thromboxane A2 receptor (TBXA2R) is a potent survival factor for triple negative breast cancers (TNBCs). Oncotarget, 7(34), 55458-55472.
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3

Western Blot Analysis of Ctbp2 and c-Myc

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Cells were washed in cold PBS and lysed in RIPA buffer (150mM NaCl, 50mM Tris HCL, pH 8.0, 1.7% NP-40, 0.17% sodium dodecyl sulfate (SDS), 0.5% Na-deoxycholate monohydrate, 5mM EDTA) containing 1 tablet of complete Mini Protease Inhibitor Cocktail /10ml (Roche). Lysates were cleared of insoluble material by centrifugation at 15,000 RPM. Proteins (30 μg) were loaded onto a Bis-Tris 4-12% gel containing NuPAGE MOPS buffer. Antibodies- Ctbp2 (Cat no. 612044, BD transduction); c-Myc (N-262; Cat no. sc-764, Santa Cruz).
Chawla A.T., Cororaton A.D., Idowu M.O., Damle P.K., Szomju B., Ellis K.C., Patel B.B, & Grossman S.R. (2018). An intestinal stem cell niche in Apc mutated neoplasia targetable by CtBP inhibition. Oncotarget, 9(65), 32408-32418.
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4

Western Blotting for Protein Analysis

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Western blotting was performed as follows:58 (link) CelLytic buffer (Sigma) supplemented with 1% protease inhibitor cocktail (Roche), 1% phosphatase inhibitor cocktail (Calbiochem Millipore), and 1 mM PMSF (Life Technologies) was used to lyse cells followed by loading the same amount of protein per sample on NuPAGE (Invitrogen) 4–12% Bis-Tris gradient gels and were further transferred on PVDF membranes (Invitrogen). Primary antibodies were used with the HRP immunodetection system (Life Technologies/Millipore). The following antibodies were used: Beta-actin (13E5; Cell Signaling Technology #4970, 1:10000), GNA13 (EPR5436; Abcam ab128900, 1:1000), BCL-6 (D65C10; Cell Signaling Technology #5650, 1:500), c-MYC (N-262; Santa Cruz sc-764, 1:500), and BCL-2 (7/Bcl-2; Becton Dickinson Biosciences 610539, 1:500). Uncropped western blots can be found in the Source Data file.
Caeser R., Di Re M., Krupka J.A., Gao J., Lara-Chica M., Dias J.M., Cooke S.L., Fenner R., Usheva Z., Runge H.F., Beer P.A., Eldaly H., Pak H.K., Park C.S., Vassiliou G.S., Huntly B.J., Mupo A., Bashford-Rogers R.J, & Hodson D.J. (2019). Genetic modification of primary human B cells to model high-grade lymphoma. Nature Communications, 10, 4543.
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5

Profiling Chromatin Modifications in T Helper Cells

Cited 2 times
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The ChIP assay was performed as previously published9 (link),49 (link),50 (link). Chromatin from primary polarized CD4+ TH1 cells maintained in variable IL-2 conditions was precipitated with antibodies to either Bcl-6 (C-19; Santa Cruz Biotechnology), c-Myc (N-262; Santa Cruz Biotechnology), HIF-1α (NB100-134; Novus Biologicals), or H3K9Ac (AB4441; Abcam). The purified DNA was analyzed by qPCR with the indicated primers. The experimental samples were first normalized to a standardized input DNA control then the IgG antibody control was subtracted to account for the nonspecific background, with this value graphically represented as the percent input of each sample.
Oestreich K.J., Read K.A., Gilbertson S.E., Hough K.P., McDonald P.W., Krishnamoorthy V, & Weinmann A.S. (2014). Bcl-6 directly represses the gene program of the glycolysis pathway. Nature immunology, 15(10), 957-964.
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6

Profiling Chromatin Modifications in T Helper Cells

Cited 2 times
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The ChIP assay was performed as previously published9 (link),49 (link),50 (link). Chromatin from primary polarized CD4+ TH1 cells maintained in variable IL-2 conditions was precipitated with antibodies to either Bcl-6 (C-19; Santa Cruz Biotechnology), c-Myc (N-262; Santa Cruz Biotechnology), HIF-1α (NB100-134; Novus Biologicals), or H3K9Ac (AB4441; Abcam). The purified DNA was analyzed by qPCR with the indicated primers. The experimental samples were first normalized to a standardized input DNA control then the IgG antibody control was subtracted to account for the nonspecific background, with this value graphically represented as the percent input of each sample.
Oestreich K.J., Read K.A., Gilbertson S.E., Hough K.P., McDonald P.W., Krishnamoorthy V, & Weinmann A.S. (2014). Bcl-6 directly represses the gene program of the glycolysis pathway. Nature immunology, 15(10), 957-964.
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7

Immunoblotting of Protein Expression Profiles

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Whole cell protein extracts were resolved on SDS-PAGE and proteins were transferred to nitrocellulose membranes. After blocking for 1 hour at room temperature in 5% milk in PBS/0.1% Tween-20, membranes were incubated overnight at 4℃ with the indicated primary antibodies: AR (441, 1:1000 dilution, Santa Cruz biotechnology, Inc.), AR (N-20, 1:1000 dilution, Santa Cruz biotechnology, Inc.), AR-V7 (AG10008, Mouse monoclonal antibody, 1:1000 dilution, precision antibody); AKR1C3 (A6229, 1:1000 dilution, Sigma-Aldrich, St. Louis, MO); c-Myc (N262, 1:1000 dilution, Santa Cruz biotechnology, Inc.); Tubulin (T5168, Monoclonal Anti-α-Tubulin antibody, 1:5000 dilution, Sigma-Aldrich, St. Louis, MO). Tubulin was used as loading control. Following secondary antibody incubation, immunoreactive proteins were visualized with an enhanced chemiluminescence detection system (Millipore, Billerica, MA).
Zhao J., Ning S., Lou W., Yang J.C., Armstrong C.M., Lombard A.P., D’Abronzo L.S., Evans C.P., Gao A.C, & Liu C. (2020). Cross-resistance among next generation anti-androgen drugs through the AKR1C3/AR-V7 axis in advanced prostate cancer. Molecular cancer therapeutics, 19(8), 1708-1718.
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