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Axiovison

Manufactured by Zeiss
Sourced in Germany

AxioVision is a software package designed for image acquisition, processing, and analysis. It is compatible with a range of Zeiss microscopy systems and digital cameras, enabling users to capture, manage, and analyze high-quality images.

Automatically generated - may contain errors

5 protocols using axiovison

1

TUNEL Assay of MDA-MB-231 Xenograft Tumors

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TUNEL assay of MDA-MB-231 xenograft tumor tissues was performed with DeadEnd Fluorometric TUNEL System Kit (Cat# G3250, Promega, Madison, WI) following the manufacturer’s protocol. Briefly, the frozen mouse tumor slides were washed with ice-cold PBS. Cells were fixed, permeabilized, and labeled with biotinylated nucleotide mix by terminal deoxynucleotidyl transferase (rTdT) reaction. Fragmented DNA was detected by streptavidin-conjugated fluorescence using Zeiss Axioplan 2 microscope. The AxioVison (Carl Zeiss) was used for data analysis.
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2

Endothelial Cell Response to Biomaterials

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All measurements are presented as mean ± standard deviation. The
t-test and chi-square test were used for comparison between the two groups. Intimal thickening and endothelial cell proliferation were measured using the AxioVison (Carl Zeiss Oberkochen Germany). A risk rate of less than 5% (
p < 0.05) was considered statistically significant.
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3

Immunohistochemistry of Xenograft Tumors

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Frozen MDA-MB-231 xenograft tumor slides were washed with ice-cold PBS. Cells were fixed, permeabilized, and incubated with Ki67 and γ-H2AX antibodies and fluorescent-labeled secondary antibodies. Immunostained cells were examined using Zeiss Axioplan 2 microscope (Carl Zeiss, Thornwood, NY) with a 20X objective. The AxioVison (Carl Zeiss) was used for data analysis.
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4

Immunohistochemistry of Xenograft Tumors

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Frozen MDA-MB-231 xenograft tumor slides were washed with ice-cold PBS. Cells were fixed, permeabilized, and incubated with Ki67 and γ-H2AX antibodies and fluorescent-labeled secondary antibodies. Immunostained cells were examined using Zeiss Axioplan 2 microscope (Carl Zeiss, Thornwood, NY) with a 20X objective. The AxioVison (Carl Zeiss) was used for data analysis.
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5

TUNEL Assay of MDA-MB-231 Xenograft Tumors

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TUNEL assay of MDA-MB-231 xenograft tumor tissues was performed with DeadEnd Fluorometric TUNEL System Kit (Cat# G3250, Promega, Madison, WI) following the manufacturer’s protocol. Briefly, the frozen mouse tumor slides were washed with ice-cold PBS. Cells were fixed, permeabilized, and labeled with biotinylated nucleotide mix by terminal deoxynucleotidyl transferase (rTdT) reaction. Fragmented DNA was detected by streptavidin-conjugated fluorescence using Zeiss Axioplan 2 microscope. The AxioVison (Carl Zeiss) was used for data analysis.
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