Dynamic light scattering (DLS) and zeta potential measurements were conducted using a Zetasizer Nano ZS (Malvern). Scanning electron microscopy (SEM) was conducted using a Zeiss Supra 25 Field Emission scanning electron microscope. SEM samples were prepared by gold sputtering and critical point drying. For AFM measurements, nanoparticles were observed on a freshly cleaved mica surface using an Asylum MFP 3D Bio AFM with an Olympus AC240TS probe. For transmission electron microscopy (TEM), a carbon-coated copper TEM grid (Ted Pella) was used with a JEOL 1200 EX transmission electron microscope operated at 80 kV.
Supra 25 field emission scanning electron microscope
Manufactured by Zeiss
Sourced in United States
The Supra 25 Field Emission Scanning Electron Microscope is a high-performance imaging and analytical tool designed for advanced materials research and characterization. It utilizes a field emission electron source to provide high-resolution imaging capabilities and superior analytical performance.
Automatically generated - may contain errors
Lab products found in correlation
Mfp 3d bio afm, by Olympus (2 mentions)
Ac240ts probe, by Olympus (2 mentions)
1200 ex transmission, by JEOL (2 mentions)
Hummer x sputter coater, by Anatech (2 mentions)
Orius sc1000 ccd camera, by Ametek (1 mentions)
Polybed 812 epoxy resin, by Polysciences (1 mentions)
Uct ultramicrotome, by Leica (1 mentions)
Ultra low attachment flask, by Corning (1 mentions)
Batson s no 17 plastic replica and corrosion kit, by Polysciences (1 mentions)
11 protocols using supra 25 field emission scanning electron microscope
1
Nanoparticle Characterization via Multimodal Analysis
2
Scanning Electron Microscopy of Embryos
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Embryos for SEM were fixed in 2.5% glutaraldehyde overnight at 4°C, processed using standard procedures and imaged with a Zeiss Supra 25 Field Emission Scanning Electron Microscope.
3
Microstructural Analysis of ISFIs
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The microstructures of ISFIs were analyzed using SEM imaging [16 (link)]. Drug-loaded formulations (30 µL) were individually injected into 200 mL of release medium (0.1 M PBS with 2% solutol, pH 7.4) and incubated for 7 days at 37 °C. ISFIs were subsequently collected, flash-frozen using liquid nitrogen, and lyophilized for 24 h (SP VirTis Advantage XL-70, Warminster, PA, USA). The lyophilized samples were mounted on an aluminum stub using carbon tape, and sputter-coated with 5 nm of gold–palladium alloy (60:40) (Hummer X Sputter Coater, Anatech USA, Union City, CA, USA). The coated samples were then imaged using a Zeiss Supra 25 field emission scanning electron microscope with an acceleration voltage of 5 kV, 30 µm aperture, and an average working distance of 10 mm (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA).
4
Scanning Electron Microscopy of Septin-Rod Structures
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The septin–rod mixture was addedonto a circular PEG-coated 12-mm coverslip and was fixed in 2.5% glutaraldehyde in 0.05 M sodium cacodylate (NaCo), pH 7.4, for 30 min, followed by two washes in 0.05 M NaCo (5 min each wash). Samples were post-fixed in 0.5% OsO4 cacodylate buffer for 30 min and washed three times in NaCo (5 min each wash). Samples were then incubated with 1% tannic acid for 15 min, followed by three washes in NaCo. 0.5% OsO4 was added for 15 min, followed by three washes in NaCo. Samples were then dehydrated with increasing ethanol concentrations (30% EtOH for 5 min, twice; 50% EtOH for 5 min; 75% EtOH for 5 min; and 100% EtOH for 5 min, twice followed by another 10 min incubation). Samples were incubated in transition fluid (hexamethyldisilazane) three times (incubation times: 5 min, 10 min, and 5 min), allowed to air dry, and then placed in a desiccator until sputter coating. Samples were coated in a gold/palladium alloy and then imaged on a Zeiss Supra 25 Field Emission scanning electron microscope.
5
Quantifying SARS-CoV-2 Spike Morphology
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WT or D614G infected primary cell cultures were submerged in fixative (4% paraformaldehyde, 2.5% glutaraldehyde and 0.1 M sodium cacodylate) overnight. For SEM, samples were rinsed, fixed with 1% OsO4 (Electron Microscopy Sciences) in perfluorocarbone FC-72 (Thermo Fischer) solution for 1 hour. After dehydration and mounted on aluminum planchets, samples were imaged using a Supra 25 field emission scanning electron microscope (Carl Zeiss Microscopy). For TEM, fixed samples were rinsed and post-fixed with potassium-ferrocyanide reduced osmium (1% osmium tetroxide/1.25% potassium ferrocyanide/0.1 sodium cacodylate buffer. The cells were dehydrated and embedment in Polybed 812 epoxy resin (Polysciences). The cells were sectioned perpendicular to the substrate at 70nm using a diamond knife and Leica UCT ultramicrotome. Ultrathin sections were collected on 200 mesh copper grids and stained with 4% aqueous uranyl acetate followed by Reynolds’ lead citrate. Samples were observed using a JEM-1230 transmission electron microscope operating at 80kV and images were taken using a Gatan Orius SC1000 CCD camera (Gatan). The number of spikes on each virion projection was quantified using ImageJ software. SEM images of infected cultures from 3 donors were imaged, at least 10 different micrographs (>100k X) were analyzed using the multi-point counting tool on individual virions.
6
Nanoparticle Characterization Protocol
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Dynamic light scattering and zeta potential measurements were conducted in PBS using a Zetasizer Nano ZS (Malvern). Scanning electron microscopy was conducted using a Zeiss Supra 25 Field Emission scanning electron microscope. Samples were prepared by gold sputtering and critical point drying.
7
Nanoparticle Characterization via Multimodal Analysis
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Dynamic light scattering (DLS) and zeta potential measurements were conducted using a Zetasizer Nano ZS (Malvern). Scanning electron microscopy (SEM) was conducted using a Zeiss Supra 25 Field Emission scanning electron microscope. SEM samples were prepared by gold sputtering and critical point drying. For AFM measurements, nanoparticles were observed on a freshly cleaved mica surface using an Asylum MFP 3D Bio AFM with an Olympus AC240TS probe. For transmission electron microscopy (TEM), a carbon-coated copper TEM grid (Ted Pella) was used with a JEOL 1200 EX transmission electron microscope operated at 80 kV.
8
Preparation and Characterization of Tumor Spheroids
9
Pial Collateral Vascular Casting and Analysis
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Mice were perfused with maximal vessel dilation as described above. Brain arterial vasculature was then casted using a Batson’s No 17 Plastic Replica and Corrosion Kit (Polysciences, Inc, Warrington, PA, USA). Briefly, 1 ml of Batson’s 17 was infused through the thoracic aorta. After fully curing, the brain tissue was removed using maceration solution, and the cerebral vasculature including the pial collateral regions were carefully persevered for emission scanning electron microscopy. The vasculature was observed under a Zeiss Supra 25 Field emission scanning electron microscope. Images of pial collateral and DMAs were saved for analysis of primary cilia and endothelial cell morphology. To measure the orientation of collateral endothelial cells, we use Photoshop to draw a line coordinate with the collateral axis and a second line coordinate with the endothelial cell axis (see Figure 2 , panel C), and the angle formed was measured.
10
Hydrogel-based Neural Stem Cell Culture
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Hydrogels were prepared as previously described with a cell loading density of 106–107 NSCs/mL of hydrogel (200 μL/mold, n = 3) and incubated at 37 C/5%CO2 in serum free DMEM media for 24 h. The hydrogel scaffolds were removed, flash frozen with liquid nitrogen, and lyophilized for 24 h. Lyophilized samples were subsequently sliced, mounted onto carbon tape, and sputter coated with 8 nm of gold-palladium alloy. Images were captured using a Zeiss Supra 25 field emission scanning electron microscope with a numerical aperture of 30 µm at an average working distance of 10 mm (Carl Zeiss Microscopy, LLC, Thornwood, NY, USA).
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