TWIMS-MS experiments were carried out on a Waters Synapt G2-S HDMS instrument (Milford, MA, USA). All samples were introduced via nano-electrospray ionization (nESI) using a custom-built ion source interface operated in positive ion mode. The interface was designed to accommodate nESI emitters made from Pyrex melting point capillaries (Corning, NY, USA) using a vertical micropipette puller. The capillary voltage was maintained between 0.9 – 1.5 kV. Sample cone voltage and source temperature were maintained at 10 V and 80°C, respectively. TWIMS parameters were held constant for all experiments: 60 mL/min nitrogen gas flow, 40 V wave height, and 600 m/s wave velocity. The mass spectra and TWIMS arrival time distributions (ATDs) were extracted and analyzed using DriftScope 2.7 and MassLynx 4.1 (Waters), with further analysis and graphing being performed in Igor Pro 7.0 (WaveMetrics; Lake Oswego, OR, USA) and SigmaPlot 13.0 (Systat; Chicago, IL, USA).
Synapt g2 s hdms instrument
Manufactured by Waters Corporation
Sourced in United Kingdom
The Synapt G2-S HDMS instrument is a high-resolution mass spectrometer designed for advanced analytical applications. It utilizes ion mobility separation technology to provide additional structural information about analytes, complementing the high-resolution mass data. The core function of the Synapt G2-S HDMS is to perform accurate mass measurements and ion mobility separations to enhance the analysis of complex samples.
Automatically generated - may contain errors
4 protocols using synapt g2 s hdms instrument
1
Structural Analysis of Biomolecules via TWIMS-MS
2
Drift Tube Ion Mobility-Mass Spectrometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Drift tube (DT) IM-MS measurements were performed on a modified Synapt G2-S HDMS instrument (Waters Corporation, Manchester, UK), described in detail elsewhere [15 (link)]. Direct infusion measurements were performed in positive ion mode using platinum/palladium (Pt/Pd, 80/20)-coated borosilicate capillaries prepared in-house. For nanoelectrospray ionization (nESI), typically 5 μL of sample was loaded to a capillary and electrosprayed by applying a capillary voltage of 0.6–1.1 kV.
3
Native Glycan Characterization by IM-MS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Traveling wave (TW) IM-MS
measurements were performed on a Synapt G2-S HDMS instrument (Waters
Corporation, Manchester, UK), described in detail elsewhere.25 (link) Direct infusion measurements with released native
glycans were performed in positive ion mode using platinum/palladium
(Pt/Pd, 80/20) coated borosilicate capillaries prepared in-house.
For nanoelectrospray ionization (nESI), typically 5 μL of the
sample was loaded to a capillary and electrosprayed by applying a
capillary voltage of 0.6–1.1 kV.
measurements were performed on a Synapt G2-S HDMS instrument (Waters
Corporation, Manchester, UK), described in detail elsewhere.25 (link) Direct infusion measurements with released native
glycans were performed in positive ion mode using platinum/palladium
(Pt/Pd, 80/20) coated borosilicate capillaries prepared in-house.
For nanoelectrospray ionization (nESI), typically 5 μL of the
sample was loaded to a capillary and electrosprayed by applying a
capillary voltage of 0.6–1.1 kV.
4
Linear Drift Tube IM-MS Measurements
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Linear drift tube (DT) IM-MS measurements were performed on a modified Synapt G2-S HDMS instrument (Waters Corporation, Manchester, UK), described in detail elsewhere. 22 Measurements were performed in positive and negative ion mode with platinum/palladium (Pt/Pd, 80/20) coated borosilicate capillaries prepared in-house. Prior to measurements, each sample was diluted from stock solution with methanol : water (1 : 1) to result in a final concentration of 10 µM. Salt solutions were generated by adding a 10 mg mL -1 aqueous stock solution of KCl/LiCl/NaCl to the labelled glycan solution to result in a 1 : 5 ratio (salt : glycan).
For nano-electrospray ionization (nano-ESI) typically 5 µL of sample was loaded to a capillary and electrosprayed by apply-ing a capillary voltage of 0.6-1.1 kV. Typical parameters in positive ion mode were: 60 V sampling cone voltage, 1 V source offset voltage, 30 °C source temperature, 0 V trap CE (MS) up to 30 V trap CE (MSMS), 2 V transfer CE, 3 mL min -1 trap gas flow. Ion mobility parameters were: 2.
For nano-electrospray ionization (nano-ESI) typically 5 µL of sample was loaded to a capillary and electrosprayed by apply-ing a capillary voltage of 0.6-1.1 kV. Typical parameters in positive ion mode were: 60 V sampling cone voltage, 1 V source offset voltage, 30 °C source temperature, 0 V trap CE (MS) up to 30 V trap CE (MSMS), 2 V transfer CE, 3 mL min -1 trap gas flow. Ion mobility parameters were: 2.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!