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Beta imager 2000z digital beta imaging system

Manufactured by Biospace
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Sourced in France

The Beta Imager 2000Z Digital Beta Imaging System is a device used to detect and analyze beta-emitting radioactive samples. It captures digital images of beta-particle emissions from samples placed within the system. The device provides quantitative data on the spatial distribution and intensity of beta particles.

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Lab products found in correlation

Beta vision plus, by Biospace (5 mentions) Triethylamine, by Merck Group (2 mentions) 3h microscale, by American Radiolabeled Chemicals (2 mentions) 2 methylbutane, by Merck Group (1 mentions) Cryostat, by Leica camera (1 mentions)

5 protocols using beta imager 2000z digital beta imaging system

1

Quantitative Beta Imaging Protocol

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Dried slides were made conductive by covering the free side with copper foil tape. Slides were then placed into a gas chamber containing a mixture of argon and triethylamine (Sigma-Aldrich, St. Louis, MO, USA. Cat. #BP616–500) as part of a gaseous detector system - the Beta Imager 2000Z Digital Beta Imaging System (Biospace, France) – for which there is a 0.07dpm/mm2 sensitivity limit. After the gas was well mixed and a homogenous state achieved, further exposure for 20 h yielded high-quality images. A [3H]microscale with a known amount of radioactivity (ranging from 0 to 36.3 nCi/mg) was counted with each section and used to create a standard curve; in each case the standard curve had a correlation coefficient (R) > 0.99. Quantitative analysis was performed with the program Beta-Vision Plus (BioSpace, France) for each anatomical region of interest.
Li H., Yang P., Knight W., Guo Y., Perlmutter J.S., Benzinger T.L., Morris J.C, & Xu J. (2019). The interactions of dopamine and oxidative damage in the striatum of patients with neurodegenerative diseases. Journal of Neurochemistry, 152(2), 235-251.
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2

Beta Imaging of Radioactive Samples

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Slides were made conductive by coating the free side with a copper foil tape. Slides were then placed in the sample holder and loaded into a gas chamber containing a mixture of argon and triethylamine (Sigma‐Aldrich) as part of a gaseous detector system, the Beta Imager 2000Z Digital Beta Imaging System (Biospace, France). After the gas was well mixed and a homogenous state was reached, exposure of the slides for 20 h yielded high‐quality images. A [3H]Microscale (American Radiolabeled Chemicals, St Louis, Missouri) was counted simultaneously as a reference for total radioactivity quantitative analysis. Quantitative analysis was performed using the program Beta‐Vision Plus (BioSpace, France) for anatomical regions of interest.
Xu J., Sun J., Perrin R.J., Mach R.H., Bales K.R., Morris J.C., Benzinger T.L, & Holtzman D.M. (2019). Translocator protein in late stage Alzheimer’s disease and Dementia with Lewy bodies brains. Annals of Clinical and Translational Neurology, 6(8), 1423-1434.
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3

Quantitative Beta Imaging of Radioactivity

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Dried slides were made conductive by covering the free side with copper foil tape. Slides were then placed into a gas chamber containing a mixture of argon and triethylamine (Sigma‐Aldrich, St. Louis, MO, USA, Cat. #BP616‐500) as part of a gaseous detector system – the Beta Imager 2000Z Digital Beta Imaging System (Biospace, France) – for which there is a 0.07dpm/mm2 sensitivity limit. After the gas was well mixed and a homogenous state achieved, further exposure for 20 h yielded high‐quality images. A [3H]microscale with a known amount of radioactivity (ranging from 0 to 36.3 nCi/mg) was counted with each section and used to create a standard curve; in each case the standard curve had a correlation coefficient (R) > 0.99. Quantitative analysis was performed with the program Beta‐Vision Plus (BioSpace, Paris, France) for each anatomical region of interest.
Li H., Yang P., Knight W., Guo Y., Perlmutter J.S., Benzinger T.L., Morris J.C, & Xu J. (2019). The interactions of dopamine and oxidative damage in the striatum of patients with neurodegenerative diseases. Journal of Neurochemistry, 152(2), 235-251.
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4

Quantitative Beta Particle Imaging

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Dried slides were made by covering the free side with copper foil tape. Then slides were placed into a gas chamber containing a balance of argon and triethylamine (Sigma-Aldrich, BP616-500, St. Louis, MO, USA) as part of a gaseous detector system, Beta Imager 2000Z Digital Beta Imaging System (Biospace, Nesles la Vallée, France) with a sensitivity limit of 0.07dpm/mm2. When a homogenous state was achieved, further exposure for 20 h yielded high-quality images. A [3H]microscale with known radioactivity (ranging from 0 to 36.3 nCi/mg) was counted with each section and used to establish a standard curve with a correlation coefficient of r2 > 0.99. Quantitative analysis was accomplished with the program Beta-Vision Plus (BioSpace, Nesles la Vallée, France) for each region of interest.
Li H., Knight W.C., Yang P., Guo Y., Perlmutter J.S., Morris J.C., Bateman R.J., Benzinger T.L, & Xu J. (2020). Microglia Implicated in Tauopathy in the Striatum of Neurodegenerative Disease Patients from Genotype to Phenotype. International Journal of Molecular Sciences, 21(17), 6047.
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5

Brain Glucose Uptake Quantification

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Immediately after the last extinction session, animals were injected with 2 μCi/kg of radioactive 2-deoxyglucose ([3H]-2DG, PerkinElmer) and were returned to their home cage. One hour postinjection, animals were sacrificed using cervical dislocation and their brain dissected in 2-methylbutane (Sigma), stored at −80°C, and coronally cut at 30 μm thickness using a cryostat (Leica). Slides were air dried, made conductive by coating the free side with a copper foil tape, and placed into a gaseous chamber containing a mixture of argon and triethylamine (Sigma-Aldrich) as part of the Beta Imager 2000Z Digital Beta Imaging System (Biospace). Exposure for 20 hr yielded high-quality images. A [3H]-Microscale (American Radiolabeled Chemicals) was counted simultaneously as a reference for total radioactivity quantitative analysis. Quantitative analysis was performed with the program Beta-Vision Plus (BioSpace) for each anatomical region of interest. At least six brain sections of the same rostrocaudal position were used to obtain an average [3H]-2DG uptake per mouse.
Gräff J., Joseph N.F., Horn M.E., Samiei A., Meng J., Seo J., Rei D., Bero A.W., Phan T.X., Wagner F., Holson E., Xu J., Sun J., Neve R.L., Mach R.H., Haggarty S.J, & Tsai L.H. (2014). Epigenetic Priming of Memory Updating during Reconsolidation to Attenuate Remote Fear Memories. Cell, 156(0), 261-276.
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