Worniu gal4

Manufactured by Bloomington Drosophila Stock Center

Worniu-Gal4 is a genetic tool used for targeted gene expression in Drosophila melanogaster. It is a GAL4 driver line that expresses the GAL4 transcription factor under the control of the worniu gene regulatory sequence, allowing for the expression of desired transgenes in specific cell populations during development.

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4 protocols using worniu gal4

Dual-channel Imaging of Cytoskeletal Dynamics

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UAS-Lifeact-Ruby (Bloomington stock 35545), BAC-encoded aPKC-GFP (Besson et al., 2015 (link)) and Sqh-GFP (Royou et al., 2002 (link)) transgenes were used to assess F-actin, aPKC and myosin II dynamics, respectively. Expression of Lifeact was specifically driven in nerve cells upon crossing UAS-Lifeact-Ruby to insc-Gal4 (1407-Gal4, Bloomington stock 8751) or to worniu-Gal4 (Bloomington stock 56553). The following genotypes were examined through dual channel live imaging: BAC-aPKC-GFP / Y; insc-Gal4, +/+, UAS-Lifeact-Ruby; and; worGal4, Sqh-GFP, +/+, UAS-Lifeact-Ruby.

Oon C.H, & Prehoda K.E. (2021). Phases of cortical actomyosin dynamics coupled to the neuroblast polarity cycle. eLife, 10, e66574.

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Drosophila Genetics: Fly Lines and Tools

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Flies were maintained on cornmeal-molasses medium at 25° unless otherwise stated. The collection of ENU-mutagenized Drosophila, including line 867 was a kind gift of Dr. Steven Robinow (University of Hawaii). UAS-brat, brat^ts1, brat^fs1 and brat^k06028 were obtained from Dr. Cheng-Yu Lee (University of Michigan). R9D11-mCD8-GFP was obtained from Dr. Jill Wildonger (University of Wisconsin-Madison). The following fly lines were obtained from the Bloomington Drosophila Stock Center at Indiana University: Df(2L)ED1272 (#24116), Df(2L)ED1203 (#8935), Df(2L)BSC341 (#24365), Df(2L)ED1231 (#9174), pcna-GFP (#25749), worniu-Gal4 (#56554), nSyb-Gal4 (#51635), CG15864^MB04166 (#24678), UAS-NICD (#52008), OK107-Gal4 (#854), UAS-mCD8-GFP (#5137), and insc-Gal4 (#8751). UAS-Brat-RNAi (#105054) was obtained from the Vienna Drosophila Resource Center (Dietzl et al. 2007 (link)).

Loewen C., Boekhoff-Falk G., Ganetzky B, & Chtarbanova S. (2018). A Novel Mutation in Brain Tumor Causes Both Neural Over-Proliferation and Neurodegeneration in Adult Drosophila. G3: Genes|Genomes|Genetics, 8(10), 3331-3346.

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Tissue-Specific CTCF Knockout in Drosophila

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(CTCF^KO, UAS-FLP)/TM6B heterozygotes were crossed to CTCF^KO/TM6B heterozygotes for an independently isolated CTCF^KO allele that also carried an FRT-flanked genomic CTCF rescue transgene and one of various Gal4 drivers: expressed in neuroblasts [worniu-Gal4 (Bloomington stock 56553)], mature neurons [elav-Gal4 (Bloomington stock 25750)], or muscles [Mef2-Gal4 (Bloomington stock 25756)]. Resulting non-TM6B animals were transheterozygous for CTCF^KO alleles, derived from a WT maternal germline, and expressed UAS-FLP under the control of a Gal4 driver leading to tissue-specific excision of the CTCF rescue transgene. w¹¹¹⁸ (wildtype) and CTCF^KO transheterozygous animals were used as controls.

Kaushal A., Mohana G., Dorier J., Özdemir I., Omer A., Cousin P., Semenova A., Taschner M., Dergai O., Marzetta F., Iseli C., Eliaz Y., Weisz D., Shamim M.S., Guex N., Lieberman Aiden E, & Gambetta M.C. (2021). CTCF loss has limited effects on global genome architecture in Drosophila despite critical regulatory functions. Nature Communications, 12, 1011.

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Drosophila Genetic Manipulation Protocols

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Fly lines used in this study included: w¹¹¹⁸ (used as a wild-type control, Bloomington stock #3605), the third chromosome balancer stock w*; Sb¹/TM3, P{ActGFP}JMR2, Ser¹ (referred to as TM3-GFP, Bloomington stock #4534), y¹ M{nos-Cas9.P}ZH-2A w* (referred to as nanos-Cas9, Bloomington stock #54591 provided by Fillip Port and Simon Bullock, MRC Laboratory of Molecular Biology), w*; P{GawB}OK107 ey^OK107/In(4) ci^D, ci^D pan^ciD sv^spa-pol (referred to as OK107-GAL4, Bloomington stock #854), w*; P{wor.GAL4.A}2; Dr¹/TM3, P{Ubx-lacZ.w⁺}TM3, Sb¹ (referred to as worniu-GAL4, Bloomington stock #56553), w¹¹¹⁸; P{GMR37H04-GAL4}attP2 [referred to as Scabrous (Sca)-GAL4, Bloomington stock #49969], w¹¹¹⁸; P{y[+t7.7] w[+mC]=GMR38F05-GAL4}attP2 [referred to as Neurotactin (Nrt)-GAL4, Bloomington stock #49383], y¹ w*; P{w^+mC=UAS-mCD8::GFP.L}LL5, P{UAS-mCD8::GFP.L}2 (referred to as UAS-mCD8-GFP, Bloomington stock #5137), KO121 Nopp140 gene deletion line (He et al., 2015 (link)), and the UAS-TComC4.2 Nopp140 RNAi line (Cui and DiMario, 2007 (link)). Flies were maintained in the laboratory at room temperature (22–24°C) on standard cornmeal-molasses medium. All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Baral S.S., Lieux M.E, & DiMario P.J. (2020). Nucleolar stress in Drosophila neuroblasts, a model for human ribosomopathies. Biology Open, 9(4), bio046565.

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