MBD was prepared as a precursor of microcoated diets (Table 1 ). Chitosan was purchased from Solarbio Biotechnology Co., Ltd. (Beijing, China), and the deacetylation degree was more than 90%. Chitosan coating solution were prepared in 2% glacial acetic acid at first. Then, the wall material was sprayed on the surface of the core, and the feed product was obtained after drying at 50 degrees. The process index of the coating machine was 12 m3/h air volume, 100 rpm rotary speed, 54.0°C air inlet temperature, and 0.20 sec pulse blowing time with 3.0 sec interval time. The fish were fed with MCD which processed with Chitosan coating solution (0.30% Chitosan, 0.60% Chitosan, and 0.90% Chitosan) [17 (link), 18 (link)]. All preparation was done at the Nankou Base of Chinese Academy of Agricultural Science (Beijing, China).
Chitosan
Manufactured by Solarbio
Sourced in China
Chitosan is a natural biopolymer derived from the exoskeletons of crustaceans such as shrimp and crab. It is a versatile material that finds applications in various fields, including biotechnology, medicine, and water treatment. Chitosan possesses unique properties, such as biocompatibility, biodegradability, and antimicrobial activity. This product is suitable for use in a wide range of laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Cd8 pe, by Thermo Fisher Scientific (1 mentions)
Cxcr3 fitc, by Thermo Fisher Scientific (1 mentions)
Mouse anti ki67, by Thermo Fisher Scientific (1 mentions)
Folic acid, by Merck Group (1 mentions)
Trypsin, by Thermo Fisher Scientific (1 mentions)
Mitoxantrone hydrochloride, by Merck Group (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
Curcumin, by Merck Group (1 mentions)
Span 80, by Sinopharm (1 mentions)
Liquid paraffin, by Sinopharm (1 mentions)
Polycaprolactone, by Merck Group (1 mentions)
Simulated body fluid, by Merck Group (1 mentions)
Cck 8 reagent, by Beyotime (1 mentions)
Milli q, by Merck Group (1 mentions)
Pepsin, by Merck Group (1 mentions)
Tryptic soy broth (tsb), by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle s medium dmem, by Thermo Fisher Scientific (1 mentions)
Mueller hinton agar (mha), by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Acetic acid, by Merck Group (1 mentions)
9 protocols using chitosan
1
Chitosan-Coated Microparticle Diets
2
Chitosan-based EGFP-IP-10 Delivery System
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Chitosan (deacetylation degree ≥ 90%) was purchased from Solarbio Company (Beijing, China). Folic acid was purchased from Sigma-Aldrich (Sigma, USA). The recombinant IP-10 plasmid was constructed by cloning the mouse IP-10 cDNA fragment into the N-terminal of pReceiver-MO3, which contained the enhanced green fluorescent protein (EGFP) 8 (link). Monoclonal anti-mouse CD11b-FITC (m1-70) and Ly6G-PE (RB6-8C5), CD80-FITC (16-10A1), CD86-FITC (GL1), MHCII-FITC (NIMR-4), CXCR3-FITC, CD8-PE, and anti-mouse Ki67 were purchased from eBiosciences (San Diego, USA). Chitosanase was purchased from Calbiochem (Dames, Germany). Monoclonal rabbit anti-mouse IP-10 was acquired from Boster (Wuhan, China). Fluorescent apoptosis detection kit was obtained from Roche (Roche, Switzerland). Mouse IL-12 ELISA kit was from Peprotech (Peprotech, USA) and INF-γ ELISPOT assay kit was purchased from Dakewe (Shenzhen, China).
3
Gelatin Extraction and Characterization
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Flounder bones were provided by Dalian Tianbao Green Foods Co., Ltd. (Dalian, Liaoning, China). Gelatin extractION from the flounder bones was performed by hydrolysis of type I collagen. The molecular weight was about 200 kDa, and the isoelectric point was around 6.7. The protein content of gelatin after removal of the heteroprotein was 32.53%, the gelatin’s pH was 7.4, and the proline and hydroxyproline concentrations were 385.89 and 232.35 nmol/mg, respectively. DHA algal oil powder was provided from Jiabiyou Biotechnology Co., Ltd. (Wuhan, Hubei, China). Chitosan was bought from Solarbio Science & Technology Co., Ltd (Beijing, China) (degree of deacetylation ≥90.0%). All other chemicals were purchased from Jinzhou Sinopharm Chemical Glass Co., Ltd. (Jinzhou, China), and all were of analytical grade.
4
Titanium-Polymer Composite Biomaterial
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Commercial pure Titanium (Ti; 99% pure) was provided by Baoji Titanium Industry Co. Ltd. (Baoji, Shaanxi, China). Polyethylenimine (PEI; 99%), chitosan (CS; deacetylation degree of 80%, and viscosity of 200 mPas) and proanthocyanidins (PAC) were purchased from Solarbio Life Sciences (Beijing, China). Hyaluronic acid (HA) was obtained from Bloomage Biotechnology Co. Ltd. (Jinan, Shandong, China). Minimum Essential Medium Eagle with Alpha modification (α-MEM), phosphate-buffed saline (PBS), fetal bovine serum (FBS), penicillin-streptomycin, and trypsin were acquired from Thermo Fisher Scientific (Waltham, MA, USA). Specific primary antibodies against p53, Bax, Bcl-2, and β-actin were sourced from Abcam (Cambridge, UK).
5
Chitosan-Curcumin Nanoparticle Preparation
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Chitosan (average molecular weight 20 kDa, degree of deacetylation > 90%) was purchased from Solarbio (Beijing, China). Curcumin, methoxy polyethylene glycol (2000 Da) and mitoxantrone hydrochloride were purchased from Sigma-Aldrich (Shanghai, China). 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDCI) and 4-dimethylaminopryidine (DMAP) were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). Dulbecco’s modified Eagle Medium (DMEM) was purchased from Gibco (Grand Island, NY, USA). Hepg2 cell line was from Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China).
6
Chitosan-based Wound Healing Ointment
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Liquid paraffin and Span80 (Sinopharm Chemical Reagent Co., Ltd., Beijing, China). The grade of chitosan (C6H11NO4)n was food grade, its viscosity was less than 500 mpa·s (Solarbio Science & Technology Co., Ltd., Beijing, China). Hypaphorine (Shifeng Technology Co., Ltd., Shanghai, China). HuaTuo Lithospermum burn ointment (HT) (Yichang Kangmintang Pharmaceutical Co., Ltd., Shantou, China).
7
Chitosan-Hydroxyapatite Composite Scaffold Fabrication
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The chitosan and hydroxyapatite were purchased from Solarbio (Beijing, China), polycaprolactone and simulated body fluid (SBF) from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany), reagents used for cell culture all from Thermo Fisher Scientific (Loughborough, United Kingdom), and CCK-8 reagent from Beyotime (Shanghai, China).
8
Squid Byproducts Utilization Protocol
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Squid by‐products (raw materials), consisting of a visceral ink sac, were collected directly from the pilot plant transformation line hygienically and stored at −20°C for further use. d ‐Rabinose and chitosan were purchased from Solarbio. Pepsin was obtained from Sigma‐Aldrich. Ultrapure water was prepared using a Milli‐Q (Millipore). The other chemicals used were of analytical grade and were obtained from Sinopharm Chemical Reagent Co. Ltd.
9
Synthesis and Characterization of Chitosan-PEO Nanocomposites
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Chitosan (deacetylated ≥90%, viscosity 20.0–500.0 mPas, 1 wt% in 1% Acetic acid 25°C) was purchased from Solarbio Life Science (Beijing, China). PEO (900,000 g/mol), deionized water, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT), DPPH, CCK‐8 kit (Sigma‐Aldrich, St. Louis, MO, USA), Dulbecco's modified Eagle's medium (DMEM) (Gibco, Germany), Fetal bovine serum (FBS) (Gibco, Germany), Phosphate buffered saline (PBS), trypsin, penicillin & streptomycin (Sigma‐Aldrich), silver nanoparticle and zinc oxide nanoparticle (Sigma‐Aldrich); Acetic acid (Merck, Germany), Mueller Hinton agar (Merck, Germany) and Tryptic Soy Broth (Gibco, Germany); E. coli (ATCC25922), S. aureus (ATCC25923), and P. aeruginosa (ATCC 27853), fibroblast cell (L929) was purchased from Pasteur Institute of Iran.
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