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Free and total glycerol

Manufactured by Merck Group

Free and total glycerol is a laboratory measurement that determines the amount of free glycerol and total glycerol present in a sample. It is used to analyze glycerol content in various applications.

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2 protocols using free and total glycerol

1

Plasma Metabolite Quantification Protocol

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Plasma insulin levels were determined using an ELISA kit (Crystal Chem, Downers Grove, IL). Plasma levels of total cholesterol (Thermo Electron, Louisville, CO) and free and total glycerol (Sigma) were determined using commercial colorimetric assays. Plasma true triglyceride concentrations were estimated by subtraction of free glycerol from total glycerol.
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2

Plasma Lipid and Cytokine Analysis

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Blood was collected when animals were euthanized at 17-18 weeks of age and plasma obtained by centrifugation (1,200g) at 4°C. Plasma levels of total cholesterol (Thermo Electron, Louisville, CO) and free and total glycerol (Sigma, St. Louis, MO) were determined using colorimetric assays. Plasma true triglyceride concentrations were estimated by subtraction of free glycerol from total glycerol. Plasma IL6 and Insulin levels were determined using an ELISA kit according to manufacturer’s instructions (IL6, R&D System, Minneapolis, MN; Insulin, Crystal Chem, Downers Grove, Illinois).
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