For VDE and PsbS detection, antibodies specific to the VDE and PsbS proteins (Agrisera) were used, followed by incubation with a goat antirabbit HRP-linked antibody (Cell Signaling Technology 7074). For HY5 detection, antibodies specific to HY5 proteins (Shanghai Jiayuan Bio.) were used, followed by incubation with a goat antirabbit HRP-linked antibody (Cell Signaling Technology 7074). To test its mobility, a mouse monoclonal antibody recognizing HA (Pierce 26183) was used, followed by incubation with a goat antimouse IgG antibody (Millipore, AP124P).
Hrp linked goat anti rabbit antibody
The HRP-linked goat anti-rabbit antibody is a secondary antibody used for the detection and visualization of primary rabbit antibodies in various immunoassays. It is conjugated with horseradish peroxidase (HRP), an enzyme that can catalyze a colorimetric reaction, enabling the detection of the target antigen.
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10 protocols using hrp linked goat anti rabbit antibody
Immunoblot Analysis of Plant Proteins
For VDE and PsbS detection, antibodies specific to the VDE and PsbS proteins (Agrisera) were used, followed by incubation with a goat antirabbit HRP-linked antibody (Cell Signaling Technology 7074). For HY5 detection, antibodies specific to HY5 proteins (Shanghai Jiayuan Bio.) were used, followed by incubation with a goat antirabbit HRP-linked antibody (Cell Signaling Technology 7074). To test its mobility, a mouse monoclonal antibody recognizing HA (Pierce 26183) was used, followed by incubation with a goat antimouse IgG antibody (Millipore, AP124P).
Investigating MAPK Signaling Pathways
Cinnamaldehyde Modulates Inflammatory Responses
Immunoblot Analysis of B Cell Proteins
Striatal Protein Expression Analysis
Striatal Protein Expression Analysis
Immunoblotting Analysis of Pyroptosis Proteins
Immunoblot Analysis of Inflammasome Components
Ischemic Cortex Western Blot Analysis
The following primary antibodies were used: anti-β-actin (1:1000, 4970, Cell Signaling Technology, USA), anti-IL-1β (1:1000, 31202, Cell Signaling Technology, USA), anti-IL-6 (1:1000, 12912, Cell Signaling Technology, USA), anti-H3K4me3 (1:1000; cell-signaling technology, USA), anti-H3K4me1 (1:1000, 5326, Cell Signaling Technology, USA), and anti-H3 (1:1000, 4499, Cell Signaling Technology, USA). Goat anti-rabbit HRP-linked antibody (1:1000, 7074, Cell Signaling Technology, USA) served as the secondary antibody.
Immunostaining and Western Blot Antibody Titrations
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