Ammonium acetate ch3coonh4

Adenosine was purchased from Calbiochem (Merck Millipore). Adenosine 5′-diphosphate (ADP), adenosine 5′-monophosphate (AMP), adenosine 3′,5′-cyclic monophosphate (AMPc), inosine, Erythro-9(-2-hydroxy-3-nonyl)adenine (EHNA), dipyridamol and dextrose were purchased from Sigma-Aldrich (France). FAAH enzymes were from Cayman Chemical. Ethanol was from Carlo-Erba (France). [2,8-³H]-Adenosine and [2,8-³H]-Adenosine 5′-triphosphate tetraammonium salt were obtained from Moravek Biochemicals (Brea, CA). Fluorescent probes CholEsteryl BODIPY® FL C₁₂ and CholEsteryl BODIPY® 542/563 C₁₁ were obtained from Life Technologies. Ultra-pure water was prepared using a MilliQ system (Millipore Corporation, Billerica, MA). Soluene, Ultima Gold and Hionic-Fluor were purchased from Perkin Elmer (France). Reagents and HPLC grade solvents as methanol, ethyl acetate, acetonitrile, and ammonium hydroxyde (NH₄OH) were provided by Carlo Erba (France). Potassium hydrogen phosphate (KH₂PO₄) and tetrabutylammonium chloride (TBAC) were from Fluka, ammonium acetate (CH₃COONH₄) from VWR and formic acid from Sigma-Aldrich (France). Liquid scintillator cocktails were provided from Perkin Elmer (France). Cartridges for SPE were from Phenomenex (France), and 0.45 μm Millex filters were from Millipore (France).