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Ff 01 800 12 25

Manufactured by IDEX Corporation

The Ff-01-800/12-25 is a lab equipment product. It is a flow meter that measures the flow rate of liquids or gases. The device has a measurement range of 800 l/min and can operate at pressures up to 25 bar.

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2 protocols using ff 01 800 12 25

1

Characterization of Lanthanide Nanoparticles

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The morphology of the LnNPs was analyzed on a JEM-2100F (JEOL Ltd., Japan) installed at Hanyang LINC3.0 Analytical Equipment Center (Hanyang University, Seoul, Republic of Korea) at an accelerating voltage of 200 kV. The XRD patterns of the LnNPs were characterized by an XRD-7000 diffractometer. The Fourier transform infrared (FT-IR) spectra of the LnNPs were obtained by using a Nicolet iS50 FT-IR spectrophotometer (Thermo Fisher Scientific Co., USA). The hydrodynamic diameter and zeta potential were measured by a Zetasizer Nano ZSP (Malvern Co., UK). The photoluminescence (PL) emission spectra were recorded by a spectrometer (Andor, Kymera 193i) and an intensified sCMOS detector (Andor, ISTAR-SCMOS-18F-73) under external excitation at 980 nm provided by a 980 nm laser diode (Changchun New Industries Optoelectronics Tech. CO., China, MDL-III-980-2W). The PL lifetime was measured using a photomultiplier tube detector (H10721-01; Hamamatsu, Shizuoka, Japan) attached to the spectrometer and a digital oscilloscope (Rhode & Schwarz, Munich, Germany, RTM3002) under excitation at 980 nm using pulsed laser (optical parametric oscillator (OPO) laser, Q-switched Nd-YAG laser, EKSPLA NT342). The PL emission wavelength was selectively measured using a bandpass filter (Semrock, ff-01-800/12-25) and shortpass filter (Semrock, ff-01-950/sp-25) placed in front of the detectors.
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2

Fluorescent Dye Buffering Optimization

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A well-plate sample of IRDye 800CW-2DG (LI-COR Biotechnology, NA, USA) was prepared at a constant concentration of 15 μM using seven different buffers: Intracellular pH Calibration Buffer Kit (pH 4.5, pH 5.5, pH 6.5, pH 7.5–ThermoFisher, USA) distilled H2O, PBS (pH 7), and DMSO. Laser excitation was set to 760 nm, and the emission filter used was 800 ± 10 nm (Semrock, FF01-800/12-25).
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