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Epmotion 5075vt

Manufactured by Eppendorf
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Sourced in Germany

The EpMotion 5075vt is a fully automated liquid handling system designed for a wide range of laboratory applications. It features a temperature-controlled work area and can accommodate various labware types. The EpMotion 5075vt is capable of pipetting liquids with high precision and accuracy.

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Lab products found in correlation

Powermag soil dna isolation kit, by Qiagen (1 mentions) Nucleospin 96 soil kit, by Macherey-Nagel (1 mentions) W4502, by Merck Group (1 mentions)

3 protocols using epmotion 5075vt

1

Automated DNA Extraction from Maternal and Infant Samples

Cited 1 time
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Genomic DNA was extracted from the mothers’ and infants’ samples as described in Mortensen et al., 2016 (link), using the PowerMag Soil DNA Isolation Kit optimized for epMotion (MO-BIO Laboratories, Inc, Carlsberg, CA) using the epMotion robotic platform model (EpMotion 5075VT, Eppendorf, Hamburg, Germany).
Mortensen M.S., Rasmussen M.A., Stokholm J., Brejnrod A.D., Balle C., Thorsen J., Krogfelt K.A., Bisgaard H, & Sørensen S.J. (2021). Modeling transfer of vaginal microbiota from mother to infant in early life. eLife, 10, e57051.
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2

Quantification of TMAO, Carnitine, and Related Metabolites

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Semifasted EDTA plasma TMAO, carnitine, choline, betaine, and DMG concentrations were measured using 2 previously described analytical assays (29 (link)). Briefly, sample preparation (i.e. protein precipitation, mixing, and filtration) was conducted on an EpMotion 5075vt robot fitted with a thermal mixer and vacuum manifold (Eppendorf). This was followed by UHPLC/MS-MS, performed on a Vanquish UHPLC+ system, coupled with a TSQ Quantiva triple quadrupole mass spectrometer (Thermo Scientific).
Andraos S., Jones B., Lange K., Clifford S.A., Thorstensen E.B., Kerr J.A., Wake M., Saffery R., Burgner D.P, & O'Sullivan J.M. (2020). Trimethylamine N-oxide (TMAO) Is not Associated with Cardiometabolic Phenotypes and Inflammatory Markers in Children and Adults. Current Developments in Nutrition, 5(1), nzaa179.
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3

DNA Extraction from Swab Samples

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The swab tips were submerged in 1 mL of molecular grade water (W4502, Sigma, UK), and bacteria released by 10s manual agitation. DNA extraction from 200 μl of the water was performed using NucleoSpin® 96 soil kit (740787, Macherey-Nagel, Germany) using an EpMotion 5075vt (Eppendorf, USA), following manufacturer instructions, using lysis buffer SL1 with 150 μl Enhancer SX and eluted in 70 μl elution buffer.
Rodrigues R., Chung A.P., Mortensen M.S., Fernandes M.H., Monteiro A.B., Furfuro R., Silva C.C., Manso M.C., Sørensen S.J, & Morais P.V. (2021). Temporal oral microbiome changes with brushing in children with cleft lip and palate. Heliyon, 7(3), e06513.
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