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2 protocols using anti goat secondary antibodies conjugated with alexa fluor 488 and horseradish peroxidase hrp

1

Immunoblotting Analysis of Candida Infection

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The primary antibodies are as follows: anti-C. albicans (catalogue #GTX40096, Genetex), anti-LAMP1 (catalogue #1D4B, Developmental Studies Hybridoma Bank), anti-Alix (catalogue #sc-53540 Santa Cruz Biotechnology), anti-Tsg101 (catalogue #AB83 Abcam), anti-Galectin3 (catalogue #sc-32790 Santa Cruz Biotechnology), anti-LC3 (catalogue #PA1-16930 Thermo Fisher Scientific), anti-p62 (catalogue #51145 Cell Signalling), anti-Beclin1 (catalogue #A-00023 Sigma), and anti-Cx43 (catalogue #AB0016–500 SICGEN). Anti-goat secondary antibodies conjugated with Alexa Fluor 488 and horseradish peroxidase (HRP) were purchased from Invitrogen and Life Technologies Jackson, respectively. Anti-mouse secondary antibodies conjugated with Alexa Fluor 568, Alexa Fluor 647 and HRP were purchased from Invitrogen and BioRad, respectively. Anti-rabbit secondary antibodies conjugated with Alexa Fluor 488, Alexa Fluor 647, and HRP were purchased from Invitrogen and BioRad, respectively. Anti-rat secondary antibodies conjugated with Alexa Fluor 594 and HRP were purchased from Invitrogen. Phalloidin was obtained from Sigma-Aldrich/Merck. LLOMe (L7393) and 3-MA (M9281-500MG) were purchased from Sigma-Aldrich/Merck. BAPTA-AM was obtained from Calbiochem (196419). Annexin V Alexa Fluor 488 Ready Flow Conjugate was purchased from Thermo Fisher Scientific (R37174).
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2

Immunoblotting Analysis of Candida Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols
The primary antibodies are as follows: anti-C. albicans (catalogue #GTX40096, Genetex), anti-LAMP1 (catalogue #1D4B, Developmental Studies Hybridoma Bank), anti-Alix (catalogue #sc-53540 Santa Cruz Biotechnology), anti-Tsg101 (catalogue #AB83 Abcam), anti-Galectin3 (catalogue #sc-32790 Santa Cruz Biotechnology), anti-LC3 (catalogue #PA1-16930 Thermo Fisher Scientific), anti-p62 (catalogue #51145 Cell Signalling), anti-Beclin1 (catalogue #A-00023 Sigma), and anti-Cx43 (catalogue #AB0016–500 SICGEN). Anti-goat secondary antibodies conjugated with Alexa Fluor 488 and horseradish peroxidase (HRP) were purchased from Invitrogen and Life Technologies Jackson, respectively. Anti-mouse secondary antibodies conjugated with Alexa Fluor 568, Alexa Fluor 647 and HRP were purchased from Invitrogen and BioRad, respectively. Anti-rabbit secondary antibodies conjugated with Alexa Fluor 488, Alexa Fluor 647, and HRP were purchased from Invitrogen and BioRad, respectively. Anti-rat secondary antibodies conjugated with Alexa Fluor 594 and HRP were purchased from Invitrogen. Phalloidin was obtained from Sigma-Aldrich/Merck. LLOMe (L7393) and 3-MA (M9281-500MG) were purchased from Sigma-Aldrich/Merck. BAPTA-AM was obtained from Calbiochem (196419). Annexin V Alexa Fluor 488 Ready Flow Conjugate was purchased from Thermo Fisher Scientific (R37174).
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