Genomic DNA from patients and from A549 cells was isolated with the MagNA Pure Nucleic Acid Isolation Kit using the MagNA Pure Compact instrument (Roche Applied Science, IN, USA), and amplified by polymerase chain reaction (PCR) using the oligonucleotide sense primer 5’-CTCAGTTTCCTCGCCTATGC-3’ and the anti-sense primer 5’-GAGTGAAGGCTGCGGAAGGG-3’ . PCR products were cleaned with exoSAP-IT (USB-Affimetrix, OH, USA) prior to sequencing in an ABI Prism 3100 Genetic Analyzer (Applied Biosystems-Thermo Scientific, MA, USA).
Magna pure nucleic acid isolation kit
Manufactured by Roche
Sourced in United States, Germany
The MagNA Pure Nucleic Acid Isolation Kit is a laboratory equipment product that is used for the automated isolation and purification of nucleic acids, such as DNA and RNA, from a variety of sample types. The kit utilizes magnetic bead technology to capture and separate the target nucleic acids from other cellular components, allowing for efficient extraction and purification.
Automatically generated - may contain errors
3 protocols using magna pure nucleic acid isolation kit
1
Genomic DNA Isolation and PCR Amplification
2
HLA Typing for Celiac Disease Susceptibility
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DNA was isolated from the whole blood using the Magna Pure compact instrument (Roche Diagnostics, Mannheim, Germany) and the MagNa Pure Nucleic Acid Isolation Kit (Roche Diagnostics, Mannheim, Germany) according to the manufacturer's instructions. HLA-Typing for celiac disease susceptibility genes was performed for the 195 randomly selected specimens using Sequence Specific Oligonucleotide (SSO) method. In summary, the SSO method is a DNA-based tissue typing technique using polymerase chain reaction to amplify the target DNA. The amplified product was hybridized with DQA1-specific and DQB1-specific nucleotide probes bound to fluorescently labeled beads that identified alleles encoded in the DNA sample. The process was carried out using Luminex technology in the Lab Type Kit (One lambda®, Canoga Park, USA). Reactivity patterns were interpreted with HLA Fusion software (One lambda®, Canoga Park, USA) to identify the alleles present. Further details of the procedure are available at http://www.onelambda.com .
3
Genetic Screening of PTGDR Promoter
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Genomic DNA from the A549 cell line was isolated with the MagNA Pure Nucleic Acid Isolation Kit using the MagNA Pure Compact instrument (Roche). Genomic DNA was screened for mutations in the promoter region of PTGDR by polymerase chain reaction (PCR) using the oligonucleotide sense primer 5'-CTC AGT TTC CTC GCC TAT GC-3' and the anti-sense primer 5'-GAG TGA AGG CTG CGG AAG GG-3'. PCR products were cleaned with exoSAP-IT (USB-Affimetrix) prior to sequencing in an ABI Prism 3100 Genetic Analyzer (Applied Biosystems-Thermo Scientific).
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