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2 protocols using 3 aminopropyltriethoxysaline

1

Preparation and Characterization of Aminoclay-Based Nanoparticles

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PEL was kindly provided by Daewon Pharm. Co., Ltd. (Seoul, Korea). Eudragit® RL PO and Eudragit® RS PO were obtained from Evonik Korea Ltd. (Seoul, Korea). 3-Aminopropyltriethoxysaline (ATPES, 99%), sodium taurodeoxycholate (NaTDC), and tolbutamide were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA). Lecithin (ca. 99.2% soy bean phosphatidylcholine) was purchased from Avanti lipid (USA). Magnesium chloride hexahydrate (98%) and other inorganic salts were obtained from Junsei Chemical Co., Ltd. (Tokyo, Japan). Aminoclay was prepared by the method reported by Yang et al. (2013 (link)). Acetone was purchased from Daejung chemical & metals Co., Ltd. (Gyeonggi-Do, Korea). All other chemicals were of analytical grade and all solvents were of high performance liquid chromatography (HPLC) grade.
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2

Synthesis of Multifunctional Nanoparticles

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Hydrogen tetrachloroaurate III trihydrate (HAuCl4·3H2O, 99.99%), silver nitrate (AgNO3, 99%), tetraethyl orthosilicate (Si(OC2H5)4, 999.99%), hydroxylamine hydrochloride (NH2O HCl, 94%), sodium citrate (99%), sodium borohydride (NaBH4, 99%), lead (II) acetate trihydrate (99.99%), 1-Thioglycerol (TGL, 95%), dithioglycerol (DTG, 95%), 3-mercaptopropyl trimethoxysilane (MPTMS, 95%), sodium sulfide nonahydrate (Na2S, 99.99), triethylamine ((C2H5)3N, 99%), ammonia hydroxide (NH3, 28–30%), ethanol 200 proof absolute, 3-Aminopropyl triethoxysaline (APTES, 98%), 11-Mercaptoundecanoic acid (MUA, 98%), and absolute ethanol (C2H5OH, 99.5%) where purchased from Sigma Aldrich and used as received. Argon gas (Ar, 99.98%) and nitrogen gas (N, 99.99%) was supplied by Matheson-Trigas. All solutions were prepared using ultrapure water (18 Mohm Millipore Milli-Q water).
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