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The GR1-Gal4 is a tool used in Drosophila (fruit fly) genetics research. It is a gene driver that can be used to express desired genes in a specific subset of cells in the fly. The GR1-Gal4 drives expression in a set of gustatory receptor-expressing neurons, allowing researchers to study the function of these neurons in the fly's sensory system.

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Lab products found in correlation

Nanos gal4, by Bloomington Drosophila Stock Center (1 mentions)

2 protocols using gr1 gal4

1

Drosophila Germline and Follicle Cell Expression

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w1118 flies were used as the wild-type control, except for embryo injections, when Oregon-R flies were used. The oskar RNA null genotype was described in Jenny et al. (2006) (link) and was trans-heterozygous for oskA87 (Vanzo and Ephrussi 2002 (link)) and Df(3R)pXT103 (Lehmann and Nusslein-Volhard 1986 (link)). UAS-coupled genes were expressed in the germline using pCog-Gal4:VP16 (Rorth et al. 1998 (link)), nanos-Gal4:VP16 (Rorth et al. 1998 (link)), and mat-α4-tub-Gal4:VP16 (Bloomington stock #7062) promoters. Expression in follicle cells was under the control of GR1-Gal4 (Bloomington stock #36287), and salivary gland expression was under the control of forkhead-Gal4 (Henderson and Andrew 2000 (link)).
Jambor H., Mueller S., Bullock S.L, & Ephrussi A. (2014). A stem–loop structure directs oskar mRNA to microtubule minus ends. RNA, 20(4), 429-439.
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2

Drosophila Stock Center Protocol

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RNAi lines were ordered from the Vienna Drosophila Resource Center: Patronin IR (VDRC 27654) and Dynein IR (VDRC 28054). The Rab11 RNAi line was generated by Ruth Brain (our laboratory) in the laboratory. UAS.shot-GFP, FRT42B shot3, Ubi.patronin-GFP, UAS.katanin60, GR1.Gal4, FRT19A cdc423 and FRT40A lgl4 were ordered from Bloomington Drosophila Stock Center. UAS.myoV-CT-GFP and UAS.dRip11-CT-GFP lines were gifts from Don Ready (Purdue University, USA) (Li et al., 2007 (link)). The Rab11–YFP line was a gift from Marko Brankatschk, MPI-CBG, Dresden, Germany. Kst–YFP (DGRC 115-285), Tj.Gal4 (DGRC 104-055) and FRT80B nuf (DGRC 111-536) were ordered from the Drosophila Genetic Resource Center, Kyoto. The following strains were used as in previous studies: α-spece226 (Hüelsmeier et al., 2007 (link)), kstd1113 (Campos et al., 2010 (link)), kst1 (Thomas and Kiehart, 1994 (link)), Nod.lacZ and Kin.lacZ (Clark et al., 1997 (link)). A list of Drosophila genotypes used in each figure is presented in Table S1.
Khanal I., Elbediwy A., Diaz de la Loza M.D., Fletcher G.C, & Thompson B.J. (2016). Shot and Patronin polarise microtubules to direct membrane traffic and biogenesis of microvilli in epithelia. Journal of Cell Science, 129(13), 2651-2659.
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