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Mouse lcn2 elisa kits

Manufactured by R&D Systems
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The Mouse LCN2 ELISA Kits are enzyme-linked immunosorbent assay (ELISA) kits designed to quantitatively measure the concentration of mouse lipocalin-2 (LCN2) in various biological samples, such as cell culture supernatants, serum, and plasma.

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ELISAs (144 citations) Mouse ELISA kits (102 citations) Mouse LCN2 (4 citations)

3 protocols using mouse lcn2 elisa kits

1

Quantifying LCN2 and MMP9 in Mouse Samples

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Plasma was separated from ethylenediaminetetraacetic acid (EDTA) anti-coagulated blood samples of mice by centrifugation at 3000 rpm/min for 10 min at 4 °C. Cell culture medium was subjected to a centrifugation at 2000 ×g for 10 min at 4 °C in a refrigerated centrifuge to collect supernatant. LCN2 levels were determined using mouse LCN2 ELISA kits (LCN2 in plasma: R&D Systems, Minneapolis, MN, USA; LCN2 in cell culture supernatant: Abcam, Cambridge, UK). MMP9 levels in the cell culture supernatants were detected using a mouse MMP9 ELISA kit (RayBiotech, Norcross, GA, USA).
Liu Y., Shao Y.H., Zhang J.M., Wang Y., Zhou M., Li H.Q., Zhang C.C., Yu P.J., Gao S.J., Wang X.R., Jia L.X., Piao C.M., Du J, & Li Y.L. (2023). Macrophage CARD9 mediates cardiac injury following myocardial infarction through regulation of lipocalin 2 expression. Signal Transduction and Targeted Therapy, 8, 394.
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2

Quantifying Murine Colon LCN2 Levels

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Mouse LCN2 ELISA Kits (R&D Systems) were used to measure LCN2 levels in mouse colon tissue lysates per the manufacturer’s instructions. All samples were assayed in duplicate and compared with a standard curve to quantitate expression.
Kim S.L., Shin M.W., Seo S.Y, & Kim S.W. (2022). Lipocalin 2 potentially contributes to tumorigenesis from colitis via IL-6/STAT3/NF-κB signaling pathway. Bioscience Reports, 42(5), BSR20212418.
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3

Quantifying Mouse Colon LCN2 Levels

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Mouse LCN2 ELISA Kits (R&D Systems) were used to measure LCN2 levels in mouse colon tissue lysates, per the manufacturer's instructions. All samples were assayed in duplicate and compared with a standard curve to quantitate the expression level.
Kim S.L., Shin M.W, & Kim S.W. (2022). Lipocalin 2 activates the NLRP3 inflammasome via LPS-induced NF-κB signaling and plays a role as a pro-inflammatory regulator in murine macrophages. Molecular Medicine Reports, 26(6), 358.
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